Chitin-induced systemic disease resistance in rice requires both OsCERK1 and OsCEBiP and is mediated via perturbation of cell-wall biogenesis in leaves

Abstract

SummaryChitin is a well-known elicitor of disease resistance whose recognition by plants is crucial to perceive fungal infections. Chitin can induce both a local immune response and a systemic disease resistance when provided as a supplement in soils. Unlike local immune responses, how chitin-induced systemic disease resistance is deployed has not been studied in detail.In this study, we evaluated systemic disease resistance against the fungal pathogen Bipolaris oryzae by performing a transcriptome analysis and monitoring cell-wall composition in rice plants grown in chitin-supplemented soils. We also examined the local immune response to chitin by measuring the production of reactive oxygen species in leaves.Chitins induced both local immune response and systemic disease resistance with differing requirements for the receptors OsCERK1 and OsCEBiP. Transcriptome analysis suggested that a perturbation in cell-wall biogenesis is involved in the induction of systemic disease resistance, an idea which was supported by the induction of disease resistance by treatment with a cellulose biosynthesis inhibitor and alterations of cell-wall composition.These findings suggest that chitin-induced systemic disease resistance in rice is caused by a perturbation of cell-wall biogenesis in leaves through long-distance signalling after recognition of chitins by OsCERK1 and OsCEBiP.

Production of copper nanoparticle-immobilized chitin nanofibers and their role in plant disease control

“…7) We have reported the elicitor activity of CNF that increases ROS production, and the expression of defense genes in Arabidopsis, cabbage, rice, strawberry, and tomato. [8][9][10][11] Moreover, CNF treatment was found to induce disease resistance, either locally or systemically, against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 in Arabidopsis and the fungal pathogens Alternaria brassicicola in Arabidopsis and cabbage, Bipolaris oryzae in rice, Colletotrichum fructicola in strawberry, and Fusarium oxysporum f. sp.…”

“…lycopersici in tomato. [8][9][10][11] Chitin has several promising applications in various fields, such as biomedical, food, cosmetics, pharmaceuticals, and agriculture, owing to its biocompatible and biodegradable eco-friendly biopolymer. However, chitin does not have antimicrobial properties.…”