Chitin Cryogels Prepared by Regeneration from Phosphoric Acid Solutions

Tyshkunova, Irina V.; Чухчин, Д. Г.; Gofman, I. V.; Pavlova, Ekaterina N.; Ушаков, В. А.; Vlasova, E. N.; Poshina, Daria N.; Skorik, Yury А.

doi:10.3390/ma14185191

Cited by 7 publications

(3 citation statements)

References 44 publications

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“…A cellulose crystallinity of 55.0% was measured by XRD [39] using the program described in [40]. The crab α-chitin (BioLog Heppe GmbH, Landsberg, Germany) used had M w of 5.86 × 10 5 and dispersity (Ð) of 4.3 [41]. A degree of acetylation of 0.98 was calculated using 13 C CP-MAS NMR spectroscopy [42].…”

Section: Methodsmentioning

confidence: 99%

Biophysical Characterization and Cytocompatibility of Cellulose Cryogels Reinforced with Chitin Nanowhiskers

et al. 2022

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Polysaccharide-based cryogels are promising materials for producing scaffolds in tissue engineering. In this work, we obtained ultralight (0.046–0.162 g/cm3) and highly porous (88.2–96.7%) cryogels with a complex hierarchical morphology by dissolving cellulose in phosphoric acid, with subsequent regeneration and freeze-drying. The effect of the cellulose dissolution temperature on phosphoric acid and the effect of the freezing time of cellulose hydrogels on the structure and properties of the obtained cryogels were studied. It has been shown that prolonged freezing leads to the formation of denser and stronger cryogels with a network structure. The incorporation of chitin nanowhiskers led to a threefold increase in the strength of the cellulose cryogels. The X-ray diffraction method showed that the regenerated cellulose was mostly amorphous, with a crystallinity of 26.8–28.4% in the structure of cellulose II. Cellulose cryogels with chitin nanowhiskers demonstrated better biocompatibility with mesenchymal stem cells compared to the normal cellulose cryogels.

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Section: Methodsmentioning

confidence: 99%

Biophysical Characterization and Cytocompatibility of Cellulose Cryogels Reinforced with Chitin Nanowhiskers

et al. 2022

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“…These parameters are typically set to values that provide the best structure and properties for each cryogel. For example, the optimal dissolution time is 24 h at room temperature for microcrystalline cellulose [54] and 16 h at room temperature for chitin [88]. An optimum temperature also exists for gelation and cryogelation for maximization of the pore size [89].…”

Section: Gelation and Cryoconcentration Parametersmentioning

confidence: 99%

Cellulose Cryogels as Promising Materials for Biomedical Applications

Tyshkunova

Poshina

Skorik

2022

IJMS

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The availability, biocompatibility, non-toxicity, and ease of chemical modification make cellulose a promising natural polymer for the production of biomedical materials. Cryogelation is a relatively new and straightforward technique for producing porous light and super-macroporous cellulose materials. The production stages include dissolution of cellulose in an appropriate solvent, regeneration (coagulation) from the solution, removal of the excessive solvent, and then freezing. Subsequent freeze-drying preserves the micro- and nanostructures of the material formed during the regeneration and freezing steps. Various factors can affect the structure and properties of cellulose cryogels, including the cellulose origin, the dissolution parameters, the solvent type, and the temperature and rate of freezing, as well as the inclusion of different fillers. Adjustment of these parameters can change the morphology and properties of cellulose cryogels to impart the desired characteristics. This review discusses the structure of cellulose and its properties as a biomaterial, the strategies for cellulose dissolution, and the factors affecting the structure and properties of the formed cryogels. We focus on the advantages of the freeze-drying process, highlighting recent studies on the production and application of cellulose cryogels in biomedicine and the main cryogel quality characteristics. Finally, conclusions and prospects are presented regarding the application of cellulose cryogels in wound healing, in the regeneration of various tissues (e.g., damaged cartilage, bone tissue, and nerves), and in controlled-release drug delivery.

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“…Up to now, numerous studies have successfully manipulated the porous structures of chitin-based aerogels by adjusting the pore characteristics of their hydrogels through various methods, such as controlling the chitin matrix content [13][14][15][16][17] or incorporating additives, [18][19][20][21][22] regulating acid/alkali solvents, 23,24 employing neutralization processes, 25 ultrasonication, 5 etc., followed by supercritical 2 or evaporation 26 drying techniques. But beyond those approaches, controlling the growth pattern of ice crystals during the freeze-casting process offers signicant advantages in terms of robust controllability and high stability.…”

Section: Introductionmentioning

confidence: 99%