ChiS is a noncanonical DNA-binding hybrid sensor kinase that directly regulates the chitin utilization program in<i>Vibrio cholerae</i>

Klancher, Catherine A.; Yamamoto, Shouji; Dalia, Triana N.; Dalia, Ankur B.

doi:10.1101/2020.01.10.902320

Cited by 9 publications

(39 citation statements)

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“…One major chitin-responsive regulator is the orphan hybrid sensor kinase ChiS (7,9). ChiS senses chitin indirectly through the periplasmic chitin binding protein (CBP) (9,10). In the absence of chitin, CBP represses ChiS through interactions with its periplasmic domain (9,10).…”

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confidence: 99%

“…ChiS senses chitin indirectly through the periplasmic chitin binding protein (CBP) (9,10). In the absence of chitin, CBP represses ChiS through interactions with its periplasmic domain (9,10). In the presence of chitin, the CBP-chitin complex stimulates ChiS activity (9,10).…”

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confidence: 99%

“…Thus, in the presence of chitin, ChiS is active and can facilitate expression of the chitin utilization program. Alternatively, ChiS can be genetically activated in the absence of chitin by deleting cbp (10,11). In the marine environment, V. cholerae not only senses chitin to modulate gene expression, but also the presence of other bacteria through a process termed "quorum sensing" (QS) (12).…”

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confidence: 99%

“…Though a link between chitin utilization and quorum sensing has previously been suggested, it has not been directly studied (16). To investigate regulation of the chitin utilization program in V. cholerae, most studies employ the chitobiose utilization operon (chb) (10,11,17,18). The chb operon encodes the genes required for uptake and catabolism of the chitin disaccharide chitobiose, and is highly induced in the presence of chitin oligosaccharides (7).…”

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confidence: 99%

“…Several mechanisms of chb regulation have already been identified. ChiS is the master regulator of the chitin utilization program in V. cholerae, and we have recently shown this protein is a direct transcriptional activator required for induction of the chb locus (9,10). Previous work has shown that carbon catabolite repression (CCR) can also play a role in regulating chitin responsive phenotypes including ChiS-dependent induction of chb and natural transformation (18,19).…”

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Species-specific quorum sensing represses the chitobiose utilization locus inVibrio cholerae

Klancher

Newman

Ball

et al. 2020

Preprint

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AbstractThe marine facultative pathogen Vibrio cholerae forms complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir. V. cholerae-chitin interactions are critical for the growth, evolution, and waterborne transmission of cholera. This is due, in part, to chitin-induced changes in gene expression in this pathogen. Here, we sought to identify factors that influence chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide. Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon. We also found that the levels of HapR in V. cholerae are regulated by the ClpAP protease. Furthermore, we show that the canonical quorum sensing cascade in V. cholerae regulates chb expression in a HapR-dependent manner. Through this analysis we found that signaling via the species-specific autoinducer CAI-1, but not the inter-species autoinducer AI-2, influences chb expression. This phenomenon of species-specific regulation may enhance the fitness of this pathogen in its environmental niche.ImportanceIn nature, bacteria live in multicellular and multispecies communities. Microbial species can sense the density and composition of their community through chemical cues using a process called quorum sensing (QS). The marine pathogen Vibrio cholerae is found in communities on the chitinous shells of crustaceans in its aquatic reservoir. V. cholerae interactions with chitin are critical for the survival, evolution, and waterborne transmission of this pathogen. Here, we show that V. cholerae uses QS to regulate the expression of one locus required for V. cholerae-chitin interactions.

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confidence: 99%

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confidence: 99%

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Species-specific quorum sensing represses the chitobiose utilization locus inVibrio cholerae

Klancher

Newman

Ball

et al. 2020

Preprint

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A structural model for (GlcNAc)2 translocation via a periplasmic chitooligosaccharide-binding protein from marine Vibrio bacteria

Kitaoku

Fukamizo

Kumsaoad

et al. 2021

Journal of Biological Chemistry

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Two regulatory factors of Vibrio cholerae activating the mannose-sensitive haemagglutinin pilus expression is important for biofilm formation and colonization in mice

et al. 2021

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Vibrio cholerae the causative agent of cholera, uses a large number of coordinated transcriptional regulatory events to transition from its environmental reservoir to the host intestine, which is its preferred colonization site. Transcription of the mannose-sensitive haemagglutinin pilus (MSHA), which aids the persistence of V. cholerae in aquatic environments, but causes its clearance by host immune defenses, was found to be regulated by a yet unknown mechanism during the infection cycle of V. cholerae . In this study, genomic expression library screening revealed that two regulators, VC1371 and VcRfaH, are able to positively activate the transcription of MSHA operon. VC1371 is localized and active in the cell membrane. Deletion of vc1371 or VcrfaH genes in V. cholerae resulted in less MshA protein production and less efficiency of biofilm formation compared to that in the wild-type strain. An adult mouse model showed that the mutants with vc1371 or VcrfaH deletion colonized less efficiently than the wild-type; the VcrfaH deletion mutant showed less colonization efficiency in the infant mouse model. The findings strongly suggested that the two regulators, namely VC1371 and VcRfaH, which are involved in the regulation of MSHA expression, play an important role in V. cholerae biofilm formation and colonization in mice.

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ChiS is a noncanonical DNA-binding hybrid sensor kinase that directly regulates the chitin utilization program inVibrio cholerae

Cited by 9 publications

References 57 publications

Species-specific quorum sensing represses the chitobiose utilization locus inVibrio cholerae

Species-specific quorum sensing represses the chitobiose utilization locus inVibrio cholerae

A structural model for (GlcNAc)2 translocation via a periplasmic chitooligosaccharide-binding protein from marine Vibrio bacteria

Two regulatory factors of Vibrio cholerae activating the mannose-sensitive haemagglutinin pilus expression is important for biofilm formation and colonization in mice

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