Chirality dependent corona phase molecular recognition of DNA-wrapped carbon nanotubes

Salem, Daniel P.; Landry, Markita P.; Bisker, Gili; Ahn, Ji‐Young; Kruss, Sebastian; Strano, Michael S.

doi:10.1016/j.carbon.2015.08.075

Cited by 85 publications

(83 citation statements)

References 42 publications

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“…47,55,58,62,[66][67][68][69] Pan et al 55 demonstrated a multiplexed optical sensing scheme for co-factor heme and its precursory porphyrins. 47,55,58,62,[66][67][68][69] Pan et al 55 demonstrated a multiplexed optical sensing scheme for co-factor heme and its precursory porphyrins.…”

Section: Optical Swcnt Sensors For Chemicals and Biomoleculesmentioning

confidence: 99%

Single-walled carbon nanotubes as optical probes for bio-sensing and imaging

2017

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The unique physical properties of single-wall carbon nanotubes (SWCNTs) have been exploited in novel applications in various fields including electronics and life sciences. Their photoluminescence in the near-infrared (NIR) range, where optical interference from biological tissues is minimum, has rendered them particularly attractive as optical probes in biological environments. Herein we review the use of the SWCNT NIR emission in bio-sensing and imaging.To interface the insoluble carbon nanotubes with aqueous biological environment, biomaterials and organic polymers have been widely used for non-covalently functionalizing SWCNTs. Such functionalization minimizes the toxicity of carbon nanotubes in biological and physiological environments, while maintaining its optical properties. SWCNTs have been demonstrated as both in vitro and in vivo optical sensors, targeting biologically important molecules, such as neurotransmitters and cell signaling molecules. For optical imaging, functionalized SWCNTs were used as NIR contrast agents for probing cellular processes and imaging plants and small animals.We also discuss emerging SWCNT-based super-resolution schemes. We conclude that SWCNTs are promising optical materials for basic life science research, biomedical diagnostics, and therapeutics.

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Section: Optical Swcnt Sensors For Chemicals and Biomoleculesmentioning

confidence: 99%

Single-walled carbon nanotubes as optical probes for bio-sensing and imaging

2017

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“…nIR fluorescent single SWCNTs are versatile building blocks for biosensors and demonstrate detection limits down to single molecules (9)(10)(11)(12)(13). We selected the neurotransmitter dopamine for this study because of its central role for reward control and learning and because it is one of the neurotransmitters for which volume transmission is heavily discussed (14,15).…”

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confidence: 99%

High-resolution imaging of cellular dopamine efflux using a fluorescent nanosensor array

Kruss

Salem

Vuković

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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177

258

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Intercellular communication via chemical signaling proceeds with both spatial and temporal components, but analytical tools, such as microfabricated electrodes, have been limited to just a few probes per cell. In this work, we use a nonphotobleaching fluorescent nanosensor array based on single-walled carbon nanotubes (SWCNTs) rendered selective to dopamine to study its release from PC12 neuroprogenitor cells at a resolution exceeding 20,000 sensors per cell. This allows the spatial and temporal dynamics of dopamine release, following K + stimulation, to be measured at exceedingly high resolution. We observe localized, unlabeled release sites of dopamine spanning 100 ms to seconds that correlate with protrusions but not predominately the positive curvature associated with the tips of cellular protrusions as intuitively expected. The results illustrate how directionality of chemical signaling is shaped by membrane morphology, and highlight the advantages of nanosensor arrays that can provide high spatial and temporal resolution of chemical signaling.sensors | imaging | dopamine | carbon nanotubes | chemical signaling

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“…This range covers the requisite excitation maxima to visualize fluorophores and all excitation peaks of a typical SWCNT multi-chirality sample ( Fig. 2A; Bachilo et al, 2002;Miyauchi et al, 2004;Salem et al, 2016). A shutter is used to allow illumination only during sampling.…”

Section: Methodsmentioning

confidence: 99%

“…Figure 1; Kruss et al, 2014). (B) nIR fluorescence spectrum (785 nm excitation) of (GT) 15 −SWCNT before and after addition of 100 μM dopamine in PBS (raw data; Kruss et al, 2014) (C) Deconvolution of a fluorescence spectrum of (GT) 15 -wrapped SWCNT: Original spectrum (blue curve), individual chirality contributions (dashed black curves) and the sum of the individual peaks (dashed red curve; Salem et al, 2016). SWCNT, single-walled carbon nanotubes; nIR, near infrared.…”

Section: Neurotransmitter Recognition Using Neurosensorsmentioning

confidence: 99%

Nanoparticle‐Templated Molecular Recognition Platforms for Detection of Biological Analytes

Beyene

Demirer

Landry

2016

CP Chemical Biology

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3 These authors contributed equally to this work.Molecular recognition of biological analytes with optical nanosensors provides both spatial and temporal biochemical information. A recently developed sensing platform exploits near-infrared fluorescent single-wall carbon nanotubes combined with electrostatically pinned heteropolymers to yield a synthetic molecular recognition technique that is maximally transparent through biological matter. This molecular recognition technique is known as corona phase molecular recognition (CoPhMoRe). In CoPhMoRe, the specificity of a folded polymer toward an analyte does not arise from a pre-existing polymeranalyte chemical affinity. Rather, specificity is conferred through conformational changes undergone by a polymer that is pinned to the surface of a nanoparticle in the presence of an analyte and the subsequent modifications in fluorescence readout of the nanoparticles. The protocols in this article describe a novel single-molecule microscopy tool (near-infrared fluorescence and total internal reflection fluorescence [nIRF TIRF] hybrid microscope) to visualize the CoPhMoRe recognition process, enabling a better understanding of synthetic molecular recognition. We describe this requisite microscope for simultaneous single-molecule visualization of optical molecular recognition and signal transduction. We elaborate on the general procedures for synthesizing and identifying single-walled carbon nanotube-based sensors that employ CoPhMoRe via two biologically relevant examples of single-molecule recognition for the hormone estradiol and the neurotransmitter dopamine. C 2016 by John Wiley & Sons, Inc.Keywords: fluorescence microscopy r molecular recognition r near-infrared imaging r nanoparticles r neurotransmitter r nIRF TIRF hybrid microscope r single-walled carbon nanotube (SWCNT) r screening r single molecule imaging r sensors How to cite this article: Beyene, A.G., Demirer, G.S., and Landry, M.P. 2016. Nanoparticle-templated molecular recognition platforms for detection of biological analytes Curr.

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Chirality dependent corona phase molecular recognition of DNA-wrapped carbon nanotubes

Cited by 85 publications

References 42 publications

Single-walled carbon nanotubes as optical probes for bio-sensing and imaging

Single-walled carbon nanotubes as optical probes for bio-sensing and imaging

High-resolution imaging of cellular dopamine efflux using a fluorescent nanosensor array

Nanoparticle‐Templated Molecular Recognition Platforms for Detection of Biological Analytes

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