Chiral Separations of Enantiomeric Pharmaceuticals by Capillary Electrophoresis Using Sulphobutyl Ether ?-Cyclodextrin as Isomer Selector

Stereoselective RP-HPLC determination of esmolol enantiomers in human plasma after pre-column derivatization

“…Enantioselective HPLC bioassays for blocking agents included chiral stationary phases (CSPs) [3,4], chiral derivatizing agents (CDAs) [5][6][7][8] and chiral mobile-phase additives [9,10]. The chiral derivatizing agents used in chiral separation included (1S, 2S) N-[(2-isothiocyanato)-cyclohexyl]-pivalinoyl amide, ( À )-methyl chloroformate and 2,3,4,6-tetra-O-acetyl-h-D-glucopyranosyl isothiocyanate (GITC), etc.…”

Determination of esmolol and metabolite enantiomers within human plasma using chiral column chromatography

“…While enantioselective HPLC bioassays for several other betablockers have utilized chiral stationary [12,13] and mobile [14,15] phases, methods pertaining to esmolol [5,7,8] have relied upon initial asymmetric derivatization to form diastereomeric analytes which are then differentiated by standard column chromatographic techniques [16][17][18][19][20]. In general, the latter have proven to be most effective when the asymmetric adduct also contains a chromophore Fig.…”

Coupling of acetonitrile deproteinization and salting-out extraction with acetonitrile stacking in chiral capillary electrophoresis for the determination of warfarin enantiomers

“…Enantioseparation of warfarin in CE has been demonstrated by employing a variety of chiral selectors, such as human serum albumin [11], maltodextrins [12], polymeric surfactant [13], ionic liquids [14], and ␤-cyclodextrin (␤-CD) derivatives [15][16][17][18][19][20][21]. As reported by Gareil et al [15], warfarin forms inclusion complexes with ␤-CD like many other aromatic compounds.…”

“…As reported by Gareil et al [15], warfarin forms inclusion complexes with ␤-CD like many other aromatic compounds. Up to now, a variety of ␤-CD derivatives, including uncharged CDs, like methyl-␤-CD (Me-␤-CD) [15,16], dimethyl-␤-CD (DM-␤-CD) [17] and 2-hydroxypropyl-␤-CD (2-HP-␤-CD) [16], as well as anionic CDs, like sulfobutyl ether ␤-CD (SBE-␤-CD) [18], highly sulfated ␤-CD (HS-␤-CD) [16,19,20], and novel single-isomer 6-monodeoxy-6-mono (hydroxy) alkylamino-␤-CDs (mainly used in nonaqueous media) [21] were employed in chiral separation of warfarin.…”