Chiral separations by capillary electrophoresis

Stalcup, Apryll M.

doi:10.1016/b978-044451669-5/50008-9

Cited by 8 publications

(8 citation statements)

References 148 publications

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“…On-chip paper electrophoresis consists of the procedures of buffer injection, electric field generation, and sample injection. Short RNAs move relatively faster than long RNAs due to the inverse relationship between the electrophoretic mobility and the molecular size [36]. Force balance between electrophoresis and electroosmosis governs the movement of RNA bands within the separation channel [37].…”

Section: Resultsmentioning

confidence: 99%

On-chip Paper Electrophoresis for Ultrafast Screening of Infectious Diseases

Kang

et al. 2021

BioChip J

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The outbreak of new viral strains promotes advances in universal diagnostic techniques for detecting infectious diseases with unknown viral sequence. Long double-stranded RNA (dsRNA), a hallmark of infections, serves as a virus marker for prompt detection of viruses with unknown genomes. Here, we report on-chip paper electrophoresis for ultrafast screening of infectious diseases. Negatively charged RNAs pass through the micro and nanoscale pores of cellulose in order of size under an external electric field applied to the paper microfluidic channel. Quantitative separation of long dsRNA mimicking poly I:C was analyzed from 1.67 to 33 ng·μL−1, which is close to the viral dsRNA concentration in infected cells. This paper-based capillary electrophoresis chip (paper CE chip) can provide a new diagnostic platform for ultrafast viral disease detection at the point-of-care (POC) level.

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Section: Resultsmentioning

confidence: 99%

On-chip Paper Electrophoresis for Ultrafast Screening of Infectious Diseases

Kang

et al. 2021

BioChip J

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“…From the perspective of sample treatment, this allows for an active and precise separation of contaminants from the target molecule (given the unique electrophoretic properties of the different biomolecules present in blood/serum samples), dramatically reducing non-specific interactions. [15][16][17] Electrophoresis can be carried out either in free solution (capillary electrophoresis) or in a support medium (paper or gel). The former offers fast and high-resolution separation but requires strong electric fields, which limits their application at the POC (i.e.…”

Section: Conceptmentioning

confidence: 99%

Paper-Based Electrophoretic Bioassay: Biosensing in Whole Blood Operating via Smartphone

et al. 2021

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Point-of-care (PoC) tests are practical and effective diagnostic solutions for major clinical problems, ranging from the monitoring of a pandemic to recurrent or simple measurements. Although, in the recent years, a great improvement in the analytical performance of such sensors has been observed, there is still one major issue that has not been properly solved: the ability to perform adequate sample treatments. The main reason is that normally sample treatments require complicated or long procedures not adequate for a deployment at the PoC. In response, a new sensing platform, called Paper-based Electrophoretic Bioassay (PEB), that combines the key characteristics of a lateral flow assay (LFA), with the sample treatment capabilities of electrophoresis is developed. In particular, the ability of PEB to separate different types of particles and to detect human antibodies in untreated blood samples is demonstrated. Finally, in order to make the platform suitable for PoC, PEB is coupled with a smartphone that controls the electrophoresis and reads the optical signal generated. It is believed that PEB platform represents a much-needed solution for the detection of low target concentrations in complex media, solving one of the major limitations of LFA and opening new opportunities for point-of-care sensors.

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“…This approach has been used to separate and analyze the antihistamine loratadine and one of its major metabolites, desloratadine, in urine samples [97]. The same format can be used for chiral separations [98]. A recent report based on this approach used it to separate a mixture of eleven psychoactive chiral amines [99].…”

Section: Carbohydratesmentioning

confidence: 99%

Clinical and pharmaceutical applications of affinity ligands in capillary electrophoresis: A review

Zhang

Woolfork

Suh

et al. 2020

Journal of Pharmaceutical and Biomedical Analysis

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Affinity capillary electrophoresis (ACE) is a separation technique that combines a biologically-related binding agent with the separating power and efficiency of capillary electrophoresis. This review will examine several classes of binding agents that have been used in ACE and applications that have been described for the resulting methods in clinical or pharmaceutical analysis. Binding agents that will be considered are antibodies, aptamers, lectins, serum proteins, carbohydrates, and enzymes. This review will also describe the various formats in which each type of binding agent has been used in CE, including both homogeneous and heterogeneous methods. Specific areas of applications that will be considered are CE-based immunoassays, glycoprotein/glycan separations, chiral separations, and biointeraction studies. The general principles and formats of ACE for each of these applications will be examined, along with the potential advantages or limitations of these methods.

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Chiral separations by capillary electrophoresis

Cited by 8 publications

References 148 publications

On-chip Paper Electrophoresis for Ultrafast Screening of Infectious Diseases

On-chip Paper Electrophoresis for Ultrafast Screening of Infectious Diseases

Paper-Based Electrophoretic Bioassay: Biosensing in Whole Blood Operating via Smartphone

Clinical and pharmaceutical applications of affinity ligands in capillary electrophoresis: A review

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