Chiral separation of T3 enantiomers using stereoselective antibodies as a selector in micro-HPLC

Koidl, Julia; Hödl, Heike; Schmid, Martin G.; Konrad, Marlene; Petschauer, Sabine; Kostner, Gerhard M.

doi:10.1016/j.jbbm.2006.03.015

Cited by 12 publications

(10 citation statements)

References 32 publications

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“…A modified form of the Schiff base method has been used to immobilize serum albumins to amine-containing silica that has been activated with glutaraldehyde [95,112]. Similar methods have also been used to immobilize antibodies onto silica for use in chiral separations [113,114]. …”

Section: Preparation Of Protein-based Cspsmentioning

confidence: 99%

Chromatographic Studies of Protein-Based Chiral Separations

Zheng

Azaria

et al. 2016

Separations

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The development of separation methods for the analysis and resolution of chiral drugs and solutes has been an area of ongoing interest in pharmaceutical research. The use of proteins as chiral binding agents in high-performance liquid chromatography (HPLC) has been an approach that has received particular attention in such work. This report provides an overview of proteins that have been used as binding agents to create chiral stationary phases (CSPs) and in the use of chromatographic methods to study these materials and protein-based chiral separations. The supports and methods that have been employed to prepare protein-based CSPs will also be discussed and compared. Specific types of CSPs that are considered include those that employ serum transport proteins (e.g., human serum albumin, bovine serum albumin, and alpha1-acid glycoprotein), enzymes (e.g., penicillin G acylase, cellobiohydrolases, and α-chymotrypsin) or other types of proteins (e.g., ovomucoid, antibodies, and avidin or streptavidin). The properties and applications for each type of protein and CSP will also be discussed in terms of their use in chromatography and chiral separations.

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Section: Preparation Of Protein-based Cspsmentioning

confidence: 99%

Chromatographic Studies of Protein-Based Chiral Separations

Zheng

Azaria

et al. 2016

Separations

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“…This approach can be practical when the association equilibrium constant for the target with the immobilized binding agent is less than 10 5 –10 6 M −1 16 . This approach is typically used with affinity columns that are employed in chiral separations 17,18 or in studies of biological interactions that have weak-to-moderate binding strengths. 1,2,5…”

Section: General Principles Of Affinity-based Methods In Hplcmentioning

confidence: 99%

“…11,12 In addition, antibodies have been used in HPLC columns as chiral stationary phases and with direct detection for such analytes as R/S -abscisic acid, various D/L-amino acids, ( R, R )- or ( S, S )-finrozole, D/L–hydroxy acids, D/L-lactic acid, S -methamphetamine, and L-triiodothyronine. 17,18,21,22…”

Section: Affinity Methods In Hplc Using Antibodiesmentioning

confidence: 99%

High performance affinity chromatography and related separation methods for the analysis of biological and pharmaceutical agents

Zhang

Rodriguez

et al. 2018

Analyst

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The last few decades have witnessed the development of many high-performance separation methods that use biologically related binding agents. The combination of HPLC with these binding agents results in a technique known as high performance affinity chromatography (HPAC). This review will discuss the general principles of HPAC and related techniques, with an emphasis on their use for the analysis of biological compounds and pharmaceutical agents. Various types of binding agents for these methods will be considered, including antibodies, immunoglobulin-binding proteins, aptamers, enzymes, lectins, transport proteins, lipids, and carbohydrates. Formats that will be discussed for these methods range from the direct detection of an analyte to indirect detection based on chromatographic immunoassays, as well as schemes based on analyte extraction or depletion, post-column detection, and multi-column systems. The use of biological agents in HPLC for chiral separations will also be considered, along with the use of HPAC as a tool to screen or study biological interactions. Various examples will be presented to illustrate these approaches and their applications in fields such as biochemistry, clinical chemistry, and pharmaceutical research.

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“…The antibodybased CSPs were applied to enantioseparations of amphetamine and methamphetamine. Anti-3,3 ,5-triiodo-l-thyronine (anti-l-T 3 ) polyclonal antibodies were immobilized onto amino-silica gels modified with glutaraldehyde [46]. The enantiomers of T 3 were baseline separated under mild continuous isocratic elution conditions using plain phosphate buffer, pH 7.4.…”

Section: Antibodiesmentioning

confidence: 99%