Chiral Separation of Carboprost Isomers by Normal Phase LC Using Amylose Chiral Stationary Phase

Analysis of docosahexaenoic acid hydroperoxide isomers in mackerel using liquid chromatography–mass spectrometry

“…Most of the chromatograms of PC 16:0/22:6;OOH isomers clearly showed a single peak, yet that of PC 16:0/22:6;4OOH had additional peaks besides the peak of the isomer, which might be attributed to the relatively higher collision energy set for the MRM pair (44 eV). The normal-phase LC condition was seemingly able to separate their DHA;OOH-positional isomers on the glycerol backbone and/or diastereomeric structure 19 , 31 – 33 , which is described in detail in Fig. 4 .…”

Pharmaceuticals and Related Drugs

“…A second noted advantage is that it exhibits separation properties that are complementary to other materials. Representative examples of other chiral assays that have been designed for other specific compounds or groups of compounds are given in Table − .…”

Separation and preparative purification ofl‐ andd‐valine ester: Diastereomeric conjugates of atazanavir using a combination of 2‐propanol and acetonitrile in reversed‐phase high‐performance liquid chromatography

“…While the separation of enantiomers is usually achieved via the formation of a diastereomeric complex involving differential chiral interactions at the chiral stationary phase, diastereomers can mostly be separated using a chiral chromatography due to differences in physical and chemical properties [1,[3][4][5]. Enantiomers are usually separated by a chiral column in normal-phase liquid chromatography (NPLC) [6][7][8][9][10], reversed-phase liquid chromatography (RPLC) [11][12][13][14][15][16] or supercritical fluid chromatography (SFC) [17][18][19]. Another common approach for the separation of enantiomers by reversed-phase HPLC on achiral stationary phases (e.g., C8, C18) is to convert them into diastereomers by coupling with chiral derivatizing reagents (CDRs) [20].…”