Chiral separation of amino acids and glycyl dipeptides by chiral ligand-exchange capillary electrophoresis comparing Cu(II), Co(II), Ni(II) and Zn(II) complexes of three different sugar acids

Chiral Separations by Capillary Electrophoresis

“…Recently, Gübitz's group investigated the possibility of using copper, cobalt, nickel, and zinc complexes of three sugar acids-d-gluconic acid, d-saccharic acid, and l-threonic acid-as selectors for the enantioseparation of amino acids and glycyl peptides [126]. Cu(II), the most frequently used metal ion in ligand exchange CE, was also found to lead to the best separations, in combination with either saccharic acid or threonic acid.…”

Investigation of Enantiomeric Separation of Chiral Drugs by CE Using Cu(II)–Clindamycin Complex as a Novel Chiral Selector

“…The advantages of CE, compared with other chromatographic methods, are that it is a simple, rapid, and practical method for providing high separation efficiency that requires small amounts of samples and reagents. Different types of chiral selectors, including cyclodextrins (CDs) and their derivatives [8][9][10], polysaccharides [11,12], antibiotics [13][14][15][16][17][18], and metal ion complexes [19][20][21][22][23], have been successfully applied as chiral additives in CE for chiral separations.…”

Enantioresolution of Amino Acids: A Decade’s Perspective, Prospects and Challenges

“…The representative measurement of racemic alanine is shown. Figure taken from[50] with permissionChiral Ligand-Exchange Capillary Electrophoresis (CLE-CE)Giuffrida et al[29] compiled literature on ligand-exchange capillary electrophoresis (LE-CE) and capillary electrophoresis (CE) coupled on line with mass spectrometry highlighting the progresses in chiral recognition and separation of AA enantiomers by capillary electromigration techniques, using different chiral selectors, especially cyclodextrins, covering the literature published from January 2010 to March 2014.Using the principle of ligand-exchange, Höld et al[83] made a comparative study on the application of Cu(II), Co(II), Ni(II) and Zn(II) complexes of d-gluconic acid, d-saccharic acid and l-threonic acid as chiral selectors for enantioseparation of aromatic AAs by CLE-CE. In terms of high resolution, the most suitable complexing agents were Cu(II) with saccharic acid and threonic acid, Co(II) with gluconic acid and Ni(II) with gluconic acid, while Zn(II) did not show any positive results with all three sugar acids.…”