Chiral separation of acidic compounds using an O-9-(tert-butylcarbamoyl)quinidine functionalized monolith in micro-liquid chromatography

Liquid chromatographic enantiomer separations applying chiral ion-exchangers based on Cinchona alkaloids

“…Monolithic columns functionalized with QD were employed for the enantiomeric resolution of N-derivatized amino acids [66,67]. When the apparent pH was raised from 4.3 to 6.3, both the retention factor and resolution were enhanced [67]. In another study, when the apparent pH was increased from 5 to 6 increased retention, selectivity and resolution were found, similar to the results mentioned earlier [66].…”

“…Carbamoylated QD-functionalized monolithic columns were prepared and tested in the enantioseparations of N-derivatized amino acids under micro-LC conditions using ammonium acetate or formate and ACN as eluent [63,66,67]. The higher the ACN content applied in the mobile phase the lower the retention was, which suggests a hydrophobic retention contribution for the monolithic CSPs.…”

“…Enantioselectivity and resolution were also significantly affected by the pH and both of them were improved at higher pHs. Monolithic columns functionalized with QD were employed for the enantiomeric resolution of N-derivatized amino acids [66,67]. When the apparent pH was raised from 4.3 to 6.3, both the retention factor and resolution were enhanced [67].…”

An insight into chiral monolithic stationary phases for enantioselective high‐performance liquid chromatography applications

“…The monolithic column displayed good permeability, morphology, reproducibility, high mechanical stability, and satisfactory separation efficiency of forty‐seven N ‐derivatized amino acids in µ‐LC (Figure ). The results of comparing between the poly(MBQD‐ co ‐HEMA‐ co ‐EDMA) and poly(MQD‐ co ‐HEMA‐ co ‐EDMA) monoliths confirmed higher enantioselectivity and diastereoselective for the new MBQD monomer with a bulkier tert ‐butyl carbamate residue on quinidine against MQD monomer bearing a linear alkyl chain at the carbamate group .…”

Organic polymer‐based monolithic capillary columns and their applications in food analysisψ

“…In addition to the HILIC mode, the PMC could also be applied to RP separations. Wang et al reported the preparation of a chiral HILIC PMC for the enantioseparation of N-derivatized amino acids (AAs) and peptides in micro-LC [43]. After preparation, the poly(2-hydroxyethyl methacrylate-co-EDMA) was modified with O-9-(tert-butylcarbamoyl) quinidine as a chiral selector.…”