Chiral separation and determination of R/S-methamphetamine and its metabolite R/S-amphetamine in urine using LC–MS/MS

Wang, Ting; Shen, Biao; Shi, Yan; Xiang, Ping; Yu, Zhiguo

doi:10.1016/j.forsciint.2014.11.009

Cited by 43 publications

(25 citation statements)

References 45 publications

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“…Using this approach, an average sample analysis time of 6.4 min could be achieved. Compared to a conventional sequential analysis with the same HPLC system, this multiple-injection-approach significantly increases sample throughput, resulting in a shorter analysis time than the existing methods for stereoselective determination of AM in urine [6,[14][15][16][17][18][19][25][26][27][28][29]. Other approaches such as parallel analysis with several columns would require additional HPLC columns, additional pumps and a second valve.…”

Section: Methods Development and Analytical Performancementioning

confidence: 99%

“…Using chiral stationary phases eliminates the need for tedious and time-consuming derivatization steps, enabling direct enantioseparation. Chiral LC methods using MS, UV or fluorescence detection have been reported for the stereoselective analysis of AM in hair [11][12][13] and urine [14][15][16]. AM enantiomers have also been separated by capillary electrophoresis (CE) using chiral selectors as mobile phase additives and MS or UV detection [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Accelerated quantification of amphetamine enantiomers in human urine using chiral liquid chromatography and on-line column-switching coupled with tandem mass spectrometry

et al. 2016

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Amphetamine (AM) is a powerful psychostimulant existing in two enantiomeric forms. Stereoselective analysis of AM in biosamples can assist clinicians and forensic experts in differentiating between abuse of illicitly synthesized racemic AM and ingestion of pharmaceutical AM formulations containing either S-AM or different proportions of the S-and R-enantiomers. Therefore, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying AM enantiomers in urine was newly developed. The method comprised dilution with water, followed by injection of the diluted sample onto an achiral C18 trapping column for purification and subsequent backflush elution to a chiral Lux 3 µm AMP LC column by means of a switching valve. An isocratic mobile phase of 25% acetonitrile in 0.1 M aqueous ammonia was used for enantiomeric separation. Injection, cleanup and backflush of the next sample were performed before the previous sample had eluted from the analytical column, thus enabling simultaneous enantioseparation of up to three samples within the analytical column. This novel chromatographic concept allowed for increased sample throughput by accelerating both the sample preparation and the LC analysis. Analyte detection was accomplished by electrospray ionization in positive ion mode and selected reaction monitoring using a triple-stage quadrupole mass spectrometer. The method was successfully validated through assessment of its linearity, lower limit of quantification, accuracy and precision, selectivity, matrix effect, carryover, dilution integrity, and re-injection reproducibility. Linearity ranged from 0.05 -25 mg/L for both enantiomers. Proof of the method included analysis of urine samples obtained from drug abusers and patients receiving an S-AM prodrug.

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Section: Methods Development and Analytical Performancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Accelerated quantification of amphetamine enantiomers in human urine using chiral liquid chromatography and on-line column-switching coupled with tandem mass spectrometry

et al. 2016

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“…In three methodologically robust RCTs, exercise therapy was found to yield significant improvement in methamphetamine withdrawal symptoms such as craving as well as accompanying symptoms such as anxiety and depression (16)(17)(18). Professionally led, structured exercise programs conduced several times a week, with varying intensity, were studied, as well as an exercise program conducted by the patients themselves.…”

Section: Further Treatmentsmentioning

confidence: 99%

Methamphetamine-Related Disorders

Härtel-Petri¹,

Hamdorf²,

Havemann-Reinecke³

et al. 2017

Deutsches Ärzteblatt International

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“…GC methods involve complicated and time consuming pre-column derivatization steps [3] [8] [12]- [14]. Liquid chromatography mass spectrometry (LC-MS) has gained more and more popularity for drug analysis because it is generally faster, easy to use and has better sensitivity and specificity [22] [23]. Chiral additive in mobile phase is usually not compatible for mass spectrometry detection [20] [25].…”

Section: Introductionmentioning

confidence: 99%

“…Direct chiral separation using chiral stationary phase without previous derivatization is a convenient choice for LC-MS analysis. The published methods with this approach had poor sensitivity and very long acquisition time [17] [18] [23]. We are presenting a fast and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) chiral analysis method that can be used for the differentiation of the enantiomers of four compounds after an easy extraction from equine plasma and urine.…”

Section: Introductionmentioning

confidence: 99%

Fast and Sensitive Chiral Analysis of Amphetamines and Cathinones in Equine Urine and Plasma Using Liquid Chromatography Tandem Mass Spectrometry

Wang

Hartmann-Fischbach

Krueger

et al. 2015

AJAC

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A simple, rapid, sensitive and reproducible method for enantiomer analysis of methamphetamine, amphetamine, cathinone and methcathinone was developed and validated. The compounds were extracted from equine plasma and urine using a fast liquid-liquid extraction procedure. Only one milliliter plasma and one hundred microliter urine sample is needed for analysis. The extraction procedure had good recovery (>70%) and the matrix effect was negligible. Enantiomer differentiation and confirmation were achieved using liquid chromatography with chiral stationary phase and mass spectrometry detection. The method demonstrated excellent reproducibility with intraday and inter-day precision of lower than 5%. The lower limits of detection for all of the compounds studied here were at low pg/mL level for both plasma and urine. This is the first report of the analysis of four chiral compounds in equine plasma and urine. Routine application was demonstrated for (S)-and (R)-enantiomer differentiation.

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Chiral separation and determination of R/S-methamphetamine and its metabolite R/S-amphetamine in urine using LC–MS/MS

Cited by 43 publications

References 45 publications

Accelerated quantification of amphetamine enantiomers in human urine using chiral liquid chromatography and on-line column-switching coupled with tandem mass spectrometry

Accelerated quantification of amphetamine enantiomers in human urine using chiral liquid chromatography and on-line column-switching coupled with tandem mass spectrometry

Methamphetamine-Related Disorders

Fast and Sensitive Chiral Analysis of Amphetamines and Cathinones in Equine Urine and Plasma Using Liquid Chromatography Tandem Mass Spectrometry

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