Chiral Micellar Electrokinetic Chromatography

Salido-Fortuna, Sandra; Castro-Puyana, María; Marina, Marı́a Luisa

doi:10.1016/j.chroma.2020.461383

Cited by 30 publications

(27 citation statements)

References 154 publications

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“…Moreover, they should form a homogeneous micellar phase throughout the BGE that is compatible with the method of detection and have a low viscosity [144]. The enantioselectivity is determined by the type of interactions that take place, which are based on the properties of the analyte [140]. The solute either interacts with the surface of the micelle via electrostatic or dipole interactions, acts as a co‐surfactant and incorporates itself into the supramolecular micelle structure, or partitions into the hydrophobic interior of the micelle.…”

Section: Chiral Ekcmentioning

confidence: 99%

“…To achieve enantioseparation in MEKC, systems using a single type of chiral surfactant or mixtures of both chiral and achiral surfactants can be employed. Chiral selectors are also often combined with micellar PSPs to enhance separation power, which is considered one of the most effective approaches for achieving enantiomeric separations by MEKC [140]. Following this strategy, both chiral as well as achiral micelles can be employed.…”

Section: Chiral Ekcmentioning

confidence: 99%

“…Taking advantage of these extensive developments, chiral MEKC has since been applied in the analysis of food, biological samples, pharmaceuticals, and chiral analytes relevant in forensic and environmental analysis, among other categories [140]. For a comprehensive summary of the current status of MEKC and its future potential, the reader is referred to a recent review from 2020 by Salido‐Fortuna et al., where MEKC enantioseparation principles, separation strategies, mechanistic studies on chiral recognition, preconcentration techniques, and its application in the analysis of real samples are discussed [140].…”

Section: Chiral Ekcmentioning

confidence: 99%

“…Nowadays, the most frequently used surfactants suitable for chiral MEKC are chiral bile salts, polymeric (molecular) micelles, and achiral surfactants such as SDS that are used in combination with a chiral selector [140]. MEKC using chiral bile salts benefits from their commercial availability [145]; mainly sodium cholate, sodium deoxycholate, sodium taurocholate, and sodium deoxytaurocholate have been employed [140]. The use of other nonbile chiral surfactants has also been successfully reported, including amino acid based surfactants [146] and vesicle-forming cationic surfactants [147].…”

Section: Surfactantsmentioning

confidence: 99%

“…In chiral MEKC, chiral recognition is based on the partitioning of the chiral analyte between a micellar PSP and the surrounding BGE [140]. Micelles are formed upon adding surfactant monomers to an aqueous solution above their CMC.…”

Section: Surfactantsmentioning

confidence: 99%

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Past, present, and future developments in enantioselective analysis using capillary electromigration techniques

2020

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Past, present, and future developments in enantioselective analysis using capillary electromigration techniques Enantioseparation of chiral products has become increasingly important in a large diversity of academic and industrial applications. The separation of chiral compounds is inherently challenging and thus requires a suitable analytical technique that can achieve high resolution and sensitivity. In this context, CE has shown remarkable results so far. Chiral CE offers an orthogonal enantioselectivity and is typically considered less costly than chromatographic techniques, since only minute amounts of chiral selectors are needed. Several CE approaches have been developed for chiral analysis, including chiral EKC and chiral CEC. Enantioseparations by EKC benefit from the wide variety of possible pseudostationary phases that can be employed. Chiral CEC, on the other hand, combines chromatographic separation principles with the bulk fluid movement of CE, benefitting from reduced band broadening as compared to pressure-driven systems. Although UV detection is conventionally used for these approaches, MS can also be considered. CE-MS represents a promising alternative due to the increased sensitivity and selectivity, enabling the chiral analysis of complex samples. The potential contamination of the MS ion source in EKC-MS can be overcome using partial-filling and counter-migration techniques. However, chiral analysis using monolithic and open-tubular CEC-MS awaits additional method validation and a dedicated commercial interface. Further efforts in chiral CE are expected toward the improvement of existing techniques, the development of novel pseudostationary phases, and establishing the use of chiral ionic liquids, molecular imprinted polymers, and metalorganic frameworks. These developments will certainly foster the adoption of CE(-MS) as a well-established technique in routine chiral analysis.

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Section: Chiral Ekcmentioning

confidence: 99%

Section: Chiral Ekcmentioning

confidence: 99%

Section: Chiral Ekcmentioning

confidence: 99%

Section: Surfactantsmentioning

confidence: 99%

Section: Surfactantsmentioning

confidence: 99%

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Past, present, and future developments in enantioselective analysis using capillary electromigration techniques

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Molecular Separation by Using Active and Passive Microfluidic chip Designs: A Comprehensive Review

Ebrahimi,

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et al. 2023

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Separation and identification of molecules and biomolecules such as nucleic acids, proteins, and polysaccharides from complex fluids are known to be important due to unmet needs in various applications. Generally, many different separation techniques, including chromatography, electrophoresis, and magnetophoresis, have been developed to identify the target molecules precisely. However, these techniques are expensive and time consuming. “Lab‐on‐a‐chip” systems with low cost per device, quick analysis capabilities, and minimal sample consumption seem to be ideal candidates for separating particles, cells, blood samples, and molecules. From this perspective, different microfluidic‐based techniques have been extensively developed in the past two decades to separate samples with different origins. In this review, “lab‐on‐a‐chip” methods by passive, active, and hybrid approaches for the separation of biomolecules developed in the past decade are comprehensively discussed. Due to the wide variety in the field, it will be impossible to cover every facet of the subject. Therefore, this review paper covers passive and active methods generally used for biomolecule separation. Then, an investigation of the combined sophisticated methods is highlighted. The spotlight also will be shined on the elegance of separation successes in recent years, and the remainder of the article explores how these permit the development of novel techniques.

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Chiral Micellar Electrokinetic Chromatography

Cited by 30 publications

References 154 publications

Past, present, and future developments in enantioselective analysis using capillary electromigration techniques

Past, present, and future developments in enantioselective analysis using capillary electromigration techniques

Cyclodextrins

Molecular Separation by Using Active and Passive Microfluidic chip Designs: A Comprehensive Review

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