Chiral Determination of Salbutamol, Salmeterol and Atenolol by Two-Dimensional LC–LC: Application to Urine Samples

Yang, Yung Hun; Rosales-Conrado, Noelia; Guillén-Casla, Vanesa; Pérez-Arribas, L.V.; Polo-Díez, L.M.

doi:10.1007/s10337-012-2353-y

Cited by 24 publications

(18 citation statements)

References 23 publications

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“…In this work, the method proposed by Yang et al . () was slightly modified for extraction of a suitable volume of water and clean‐up. Isolute® HCX cartridges were used as SPE sorbent for SAL enrichment.…”

Section: Resultsmentioning

confidence: 97%

“…Glass bottles, pre-cleaned with high purity water, were used to collect samples. Natural water samples with high organic content were collected from a local spring (Valdeavero, Madrid) and stored in the dark at À4 C. All samples were filtered through a 0.45 mm pore size nylon filter membrane prior to extraction, and treated according to the method proposed by Yang et al (2012) for urine samples, which was slightly modified in the following way: volumes of natural water between 10 and 100 mL containing spiked racemic SAL were adjusted to pH 4.5 with acetic acid and loaded onto the Isolute W HCX cartridges at 2 mL min À1 . Firstly, SPE cartridges were pre-conditioned with 1 mL of methanol and 1 mL of high purity water.…”

Section: Sample Preparation and Spe Proceduresmentioning

confidence: 99%

“…Extraction of b 2 -agonists from biosamples is usually performed by SPE using reversed-phase and mixed-mode sorbents. In this work, the method proposed by Yang et al (2012) was slightly modified for extraction of a suitable volume of water and clean-up. Isolute W HCX cartridges were used as SPE sorbent for SAL enrichment.…”

Section: Determination Of Chiral Salbutamol In Natural Watermentioning

confidence: 99%

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Determination of salbutamol by direct chiral reversed‐phase HPLC using teicoplanin as stationary phase and its application to natural water analysis

Rosales-Conrado

Dell’Aica

Pérez-Arribas

et al. 2013

Biomedical Chromatography

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A direct chiral LC-UV method was optimized for the determination of salbutamol (SAL) β2 -agonist in environmental water. Two commercially available columns were evaluated: teicoplanin Chirobiotic-T™ (150 × 2.1 mm i.d., 5 µm) and vancomycin Chirobiotic-V™ (150 × 2.1 mm i.d., 5 µm). Finally, teicoplanin chiral stationary phase was selected for SAL enantiomer resolution. In order to preserve its integrity and maintain the column performance for longer time, the use of additives such as triethylamine (TEA) in the mobile phase was avoided. Experimental design was applied to simultaneously evaluate the influence of several parameters involved in enantiomer separation and to establish the conditions for acceptable resolution and performance in short analysis time. Optimum mobile phase was methanol-20 mM ammonium acetate buffer at pH 4.5 (98:2, v/v). A solid-phase extraction procedure for sample pre-concentration and clean-up allowed the determination of chiral SAL residues in natural water samples spiked at low concentrations in the range 1.0-20 ng mL(-1) . Reproducible recoveries, between 77 and 98%, were obtained and matrix effect was negligible. Injection of sample solutions at low elution strength permitted the SAL enantioresolution in the natural water complex matrix with satisfactory sensitivity and precision.

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Section: Resultsmentioning

confidence: 97%

Section: Sample Preparation and Spe Proceduresmentioning

confidence: 99%

Section: Determination Of Chiral Salbutamol In Natural Watermentioning

confidence: 99%

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Determination of salbutamol by direct chiral reversed‐phase HPLC using teicoplanin as stationary phase and its application to natural water analysis

Rosales-Conrado

Dell’Aica

Pérez-Arribas

et al. 2013

Biomedical Chromatography

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“…Yang et al . () have simultaneously separated salbutamol, salmeterol and atenolol in human urine, and Barreiro et al . () have separated pantoprozole and lansoprozole in wastewater samples.…”

Section: Selected Applicationsmentioning

confidence: 97%

“…Other LC couplings have also been applied to plasma samples for the determination of metyrapone and its enantiomeric metyrapol metabolite (Cassiano et al ., ), warfarin and its metabolite (Uno et al ., ), and modafinil enantiomers and its metabolites modafinic acid and modafinil sulfone (Cass and Galatti, ). Some of the 2DLC systems have been used in pharmaceutical applications for the analysis of omeoprazole in aqueous matrices (Barreiro et al ., ), pantoprozole and lansoprozole in wastewater samples (Barreiro et al ., ), salbutamol, salmeterol and atenonol in urine (Yang et al ., ), and carvedilol in pharmaceuticals formulations (Medvedovici et al ., ).…”

Section: Selected Applicationsmentioning

confidence: 99%

Two‐dimensional liquid chromatography for direct chiral separations: a review

León-González

Rosales-Conrado

Pérez-Arribas

et al. 2013

Biomedical Chromatography

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Separation of enantiomers remains a challenge owing to their identical physical and chemical properties in an achiral environment, and research on specialized separation techniques such as multidimensional achiral-chiral liquid chromatography continues to resolve individual enantiomers in complex samples. Recent advances in application of multidimensional liquid chromatography applied to chiral analysis are reviewed. For this reason, benefits of achiral-chiral coupling are shown, with emphasis in applications on biological and pharmaceutical fields as well as pesticide analysis. A description of standard instrumental setup in both heart-cut and comprehensive multidimensional liquid chromatography is shown. The most broadly used chiral stationary phases for multidimensional liquid chromatography are summarized. An extensive overview of different interface designs applied to complex samples is presented.

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Determination of flavor enhancers in milk powder by one‐step sample preparation and two‐dimensional liquid chromatography

Zhang

Wang

et al. 2014

J of Separation Science

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Maltol, ethyl maltol, vanillin, and ethyl vanillin are important food additives as flavor enhancers. To quantify the four additives in milk powder, a novel 2D liquid chromatographic (2DLC) method was developed in this article. In such a 2DLC system, the target fractions eluted from the first dimensional column (C4) are stored onto the trapping column (C8) for subsequent analysis; after that, they were switched into the second dimensional column (C18) by a two-position six-port switching valve. A one-step sample preparation method was used prior to 2DLC chromatographic analysis, which was easy and convenient. After optimization of all experimental parameters, the new method was validated in terms of linearity, LODs, and LOQs, intra- and interday precision, and accuracy. A conventional single-dimensional liquid chromatographic method was also proposed in this work for comparison. In order to evaluate the applicability of the new 2DLC method, five brands of commercial milk powder samples (n = 8) were analyzed. Vanillin and ethyl vanillin were detected in two samples, respectively. It is showed that the 2DLC method is effective in quality control programs of milk powder products.

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Chiral Determination of Salbutamol, Salmeterol and Atenolol by Two-Dimensional LC–LC: Application to Urine Samples

Cited by 24 publications

References 23 publications

Determination of salbutamol by direct chiral reversed‐phase HPLC using teicoplanin as stationary phase and its application to natural water analysis

Determination of salbutamol by direct chiral reversed‐phase HPLC using teicoplanin as stationary phase and its application to natural water analysis

Two‐dimensional liquid chromatography for direct chiral separations: a review

Determination of flavor enhancers in milk powder by one‐step sample preparation and two‐dimensional liquid chromatography

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