Chiral derivatization-enabled discrimination and on-tissue detection of proteinogenic amino acids by ion mobility mass spectrometry

Abstract

The combination of chiral derivatization and ion mobility-mass spectrometry provides the first insights into the separation of 19 pairs of chiral proteinogenic d/l-amino acids in a single run and detection of chiral amino acids in complex samples.

19F NMR enantiodiscrimination and diastereomeric purity determination of amino acids, dipeptides, and amines

“…Chiral amino-group compounds are not only important biomolecules but also chemical and pharmaceutical intermediates for the synthesis of bioactive compounds. Currently, various methods have been widely used for the elucidation of absolute configuration and enantiomeric excess (ee), including NMR, [1][2][3][4][5][6][7][8][9][10][11][12] circular dichroism, [13][14][15][16][17] fluorescence, 18 SERS, 19 chromatography, 20,21 ion mobility mass spectrometry, 22 and electrochemistry. 23,24 However, optical spectroscopic techniques require pure enantiomers that are obtained by isolating each enantiomeric pair or by extracting each enantiomer from its original matrix prior to analysis.…”

Development and evaluation of a novel fluorescent chiral derivatization reagent DBD-S-M-Pro: first observation of four chiral amino acids in human hair

“…The initially used indirect method is UV detection, which was gradually phased out owing to low sensitivity. 15 Mass spectrometry is sensitive and selective, 16,17 but the need for expensive instrumentation impedes its use in chiral amino acid analysis. Fluorescence detection has become popular because it sensitively and selectively detects molecules at high quantum efficiency using low-cost instruments.…”

Chiral-Controlled Cyclic Chemiluminescence Reactions for the Analysis of Enantiomer Amino Acids

“…Therefore, the detection of chiral AAs is vital for food science, pharmaceutics, and life sciences. Chromatography, , surface-enhanced Raman spectroscopy, electrochemical sensors, , and mass spectrometry have been used in the analysis of the chiral compounds. Because of the similar structure and the interference in the complex sample, the analysis of chiral AAs is one of the important challenges.…”