Chiral Assay of Atenolol Present in Microdialysis and Plasma Samples of Rats Using Chiral CBH as Stationary Phase

Stereoselective RP-HPLC determination of esmolol enantiomers in human plasma after pre-column derivatization

“…Enantioselective HPLC bioassays for blocking agents included chiral stationary phases (CSPs) [3,4], chiral derivatizing agents (CDAs) [5][6][7][8] and chiral mobile-phase additives [9,10]. The chiral derivatizing agents used in chiral separation included (1S, 2S) N-[(2-isothiocyanato)-cyclohexyl]-pivalinoyl amide, ( À )-methyl chloroformate and 2,3,4,6-tetra-O-acetyl-h-D-glucopyranosyl isothiocyanate (GITC), etc.…”

Determination of esmolol and metabolite enantiomers within human plasma using chiral column chromatography

“…While enantioselective HPLC bioassays for several other betablockers have utilized chiral stationary [12,13] and mobile [14,15] phases, methods pertaining to esmolol [5,7,8] have relied upon initial asymmetric derivatization to form diastereomeric analytes which are then differentiated by standard column chromatographic techniques [16][17][18][19][20]. In general, the latter have proven to be most effective when the asymmetric adduct also contains a chromophore Fig.…”

Recent developments of enantioseparation techniques for adrenergic drugs using liquid chromatography and capillary electrophoresis: A review

“…2(b) that the difference in bonding between both enantiomers gives rise to chiral separation. Based on the same principle, several adrenergic drugs and other cardiovascular drugs have been chirally recognized using these CBH I CSPs [60,61]. Recently, cellobiohydrolase 58 (CBH 58) was reported and its catalytic site was similar to that of CBH I.…”