CHIP-mediated ubiquitination of Galectin-1 predicts colorectal cancer prognosis

Abstract

CHIP and Galectin-1 are associated with the development of metastasis in cancer. However, the precise roles of CHIP or Gal1 in colorectal cancer are uncertain. Here, our study explored the relationship and clinical significance of CHIP or Gal1 in CRC. CHIP or Gal1 expression was significantly decreased or up-regulated in CRC compared with adjacent noncancerous tissues by immunohistochemistry on a CRC tissue microarray, respectively. Low CHIP or high Gal1 expression significantly correlated with clinicopathological characteristics in patients, as well as with shorter overall survival. Multivariate Cox regression analysis revealed that CHIP or Gal1 expression was an independent prognostic factor for CRC patients. Moreover, CHIP associated with Gal1 has a synergistic effect on the prediction of CRC prognosis. In vitro and vivo, high CHIP or low Gal1 expression inhibit CRC growth or metastasis. Our results found that CHIP could degradate Gal1 by ubiquitination. In summary, CHIP could inhibit CRC growth or metastasis through promoting Gal1 ubiquitination and degradation by proteasome. CHIP and Gal1 expressions are novel candidate prognostic markers in CRC. A combined effect of CHIP and Gal1 as efficient prognostic indicators was found for the first time.

Galectin-1 From Cancer-Associated Fibroblasts Promotes the Invasion and Metastasis of Gastric Cancer Through TGF-β1-Induced Epithelial-Mesenchymal Transition

“…GAL-1 has been found in multiple tumor cells, including melanoma, lung cancer [30], pancreatic cancer [31], bladder cancer [32], thyroid cancer [33], cervical cancer [34], and colorectal cancer [35]. In the present study, we showed that the GAL-1 protein and LGALS1 mRNA levels in GC tissue were signi cantly higher than those in NGCT, suggesting that GAL-1/LGALS1 is associated with the malignant biological behavior of GC.…”

CHIP-dependent regulation of the actin cytoskeleton is linked to neuronal cell membrane integrity

“…Hence, ANXA2, S100A11, HLA-B, and SORT1 were all upregulated in whole cell lysates from CHIP -/cortical neurons when compared to WT cells (Figures 2H-2K). In addition, of the proteins which meet our %0.5 and R 2-fold change criterion, both Galectin-1 (0.38-fold) and FLNA (0.36-fold) are validated CHIP substrates (Paul and Ghosh, 2015;Wang et al, 2020). Likewise, previous proteomics identified ANXA5 (0.45-fold), HLA-B (0.22-fold) -as well as, FLNA (that here was below the level of detection by immunoblot but significantly changed in the MS analysis) -as putative CHIP substrates using an orthogonal substrate identification approach (Bhuripanyo et al, 2018).…”

Fibroblastic galectin-1-fostered invasion and metastasis are mediated by TGF-β1-induced epithelial-mesenchymal transition in gastric cancer

“…GAL-1 has been found in multiple tumor cells, including melanoma, lung cancer [ 31 ], pancreatic cancer [ 32 ], bladder cancer [ 33 ], thyroid cancer [ 34 ], cervical cancer [ 35 ], and colorectal cancer [ 36 ]. The results of the present study demonstrated that the GAL-1 protein and LGALS1 mRNA levels in GC tissue were significantly higher than those in NGCT, suggesting that GAL-1/ LGALS1 is associated with the malignant biological behavior of GC.…”