Chip elements for fast thermocycling

Poser, S.; Schulz, T.; Dillner, U.; Baier, V.; Köhler, J. Michael; Schimkat, D.; Mayer, G.; Siebert, A.

doi:10.1016/s0924-4247(97)01542-2

Cited by 105 publications

(70 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This requires a spectral fluorimeter and complex data analysis. Simple optics would be better suited for portable fieldable rapid PCR instruments such as microfabricated devices (Northrup et al 1993;Northrup et al 1995;Woolley et al 1996;Poser et al 1997;Daniel et al 1998). Devices of this type would suit the detection of environmental B. anthracis.…”

Section: Resultsmentioning

confidence: 99%

Fluorescent detection techniques for real-time multiplex strand specific detection of Bacillus anthracis using rapid PCR

Lee¹,

Brightwell²,

Leslie³

et al. 1999

J Appl Microbiol

View full text Add to dashboard Cite

TO N. 1999. Speed is a key area in our development of PCR assays for Bacillus anthracis. We believe that the strand specific detection of amplicons within 10 min is a realistic goal and that this will be achieved through fluorescent in-tube assays. We have used the Idaho LightCycler TM to study and develop candidate assays for B. anthracis. New strand specific fluorescent methods have been developed and a number of formats have been studied for speed and sensitivity. Internal controls have been developed as a method of improving our assay confidence. In this communication we will introduce the field of rapid PCR whilst discussing previous work in the areas described above, the development of our own rapid assay and a novel internal control system for B. anthracis. This work used PCR assays and hardware that are either commercially available, or have been previously described in open literature publications.

show abstract

Section: Resultsmentioning

confidence: 99%

Fluorescent detection techniques for real-time multiplex strand specific detection of Bacillus anthracis using rapid PCR

Lee¹,

Brightwell²,

Leslie³

et al. 1999

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…The reduction of PCR volume and the use of highly heat conducting materials as silicon allowed to reduce the times for thermocycling considerably [45]. In addition, miniaturization opened the possibility of continuous flow PCR [46][47][48][49] and of realizing highly parallelized thermocycling processes.…”

Section: Pcr In Micro Fluid Segmentsmentioning

confidence: 99%

Droplet-Based Microfluidics as a Biomimetic Principle: From PCR-Based Virus Diagnostics to a General Concept for Handling of Biomolecular Information

Köhler

2012

Microdroplet Technology

View full text Add to dashboard Cite

The ability of lithographic fabrication of microchannels for fluid transport on the one hand and the continuously decreasing critical dimensions during the development of microlithography over the last decades generated high expectations on the progress of automated processing of small amounts of substances. In particular, it was expected that large numbers of well distinguished chemical entities-atoms, molecules, nano particles-could become manipulated highly parallel and with high speed. This hope was fed by the imagination of an analogy between the electron transport in integrated circuits and the transport of chemical objects inside microfluidic networks. The experiences of the last both decades had shown that such ideas of a complete analogy are not realistic. The progress of handling of substance-coupled information is much slower as the progress of hard ware development in the electronic devices. But, beside this general disappointment, microfluidics is still promising the arise and growth-up of very important new strategies for organizing chemical systems at the microscale and for supplying functional interfaces between the complex information inside the world of molecules and particles on the one hand and electronic systems on the other hand.Whereas clouds of electrons are manipulated in digital electronic devices, clouds of molecules are transported through microchannels by a convective flow. But, the special conditions in microfluidics cause low Reynolds numbers which reflect the small ratio of channel diameter and volume flow rate to the viscous forces. The microfluidic conditions cause always a laminary structure of flow with steep flow velocity gradients and results in to very high fluidic dispersion of concentration signals if homogeneous fluids are applied. So, the principle high potential of J.M. K€ ohler

show abstract

“…1) to narrow channels (linear or serpentine) (Fig. 2), and can have a single reaction chamber or arrays of chambers for multiple simultaneous reactions (Table 1) [25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45]. Volumes inside the microchips vary over the nL to µL range, and devices with volumes as low as 12 nL have been produced [46].…”

Section: Pcr Microchip Fabrication and Designsmentioning

confidence: 99%

“…[40] Multiple silicon [24,41] silicon-glass [19,21,43,44,45] 1. Time domain PCR -In this format the reaction mixture is kept stationary and the temperature of the surrounding reaction chamber is cycled between the different temperatures.…”

Section: Pcr Microchip Fabrication and Designsmentioning

confidence: 99%

Microchip PCR

Kricka

Wilding

2003

Analytical and Bioanalytical Chemistry

145

107

View full text Add to dashboard Cite

Miniaturization of genetic tests has become an important goal. This review surveys the current progress towards the miniaturization of tests based on the polymerase chain reaction (PCR). It examines the different types of PCR microchip designs, fabrication methods,and the components of a microchip PCR device. It also discusses the problems attributable to surface chemistry of microchip components (inhibition of PCR), and the static and dynamic surface passivation strategies developed for the solution of these difficulties

show abstract

Chip elements for fast thermocycling

Cited by 105 publications

References 3 publications

Fluorescent detection techniques for real-time multiplex strand specific detection of Bacillus anthracis using rapid PCR

Fluorescent detection techniques for real-time multiplex strand specific detection of Bacillus anthracis using rapid PCR

Droplet-Based Microfluidics as a Biomimetic Principle: From PCR-Based Virus Diagnostics to a General Concept for Handling of Biomolecular Information

Microchip PCR

Contact Info

Product

Resources

About