Chip‐based duplex real‐time PCR for water quality monitoring concerning <i>Legionella pneumophila</i> and <i>Legionella</i> spp.

Reuter, Cornelia; Hentschel, Stefanie; Breitenstein, Antje; Heinrich, Eileen; Aehlig, Oliver; Henkel, Thomas; Csáki, Andrea; Fritzsche, Wolfgang

doi:10.1111/wej.12635

Cited by 5 publications

(1 citation statement)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the most ubiquitous living entities, microbial detection is pivotal in water quality monitoring, , food safety control, and medical health. − Besides, as the signal generation units in the microbial electrochemical devices, microorganisms also play an essential role in bioenergy − and biosensors. , Saccharomyces cerevisiae, since been isolated from the environment in ancient times, has been widely used in food and beverage production, biotherapeutics (probiotics), bioremediation, and production of biofuels. Even though most industrial S.…”

Section: Introductionmentioning

confidence: 99%

Three-Mediator Enhanced Collisions on an Ultramicroelectrode for Selective Identification of Single Saccharomyces cerevisiae

et al. 2022

View full text Add to dashboard Cite

Selective detection of colliding entities, especially cells and microbes, is of great challenge in single-entity electrochemistry. Herein, based on the different cellular electron transport pathways between microbes and mediators, we report a three-mediator system [K 3 Fe(CN) 6 , K 4 Fe(CN) 6 , and menadione] to achieve redox activity analysis and selective identification of single Saccharomyces cerevisiae without the usage of antibodies. K 4 Fe(CN) 6 in the three-mediator system will oxidize near the electrode surface and increase the local concentration of K 3 Fe(CN) 6 , which will promote the redox reaction of S. cerevisiae. The hydrophobic mediator�menadione�can selectively penetrate through the S. cerevisiae membrane and get access to its intracellular redox center and can further react with K 3 Fe(CN) 6 in the bulk solution. In contrast, the mediator can only get access to the bacterial membranes of Escherichia coli and Staphylococcus aureus, which results in different electrochemical collision signals between the above microbes. In the three-mediator system, upward step-like collision signals were observed in S. cerevisiae suspension, which are related to their microbial redox activity. In comparison, E. coli or S. aureus only generated downward current steps because the blockage effect of mediator diffusion suppresses their redox activities. When S. cerevisiae co-existed with E. coli or S. aureus, transients generated by both blockage and redox activity were observed. The approach enables us to trace the collision behaviors of different microbes and distinguish their simultaneous collisions, which is the foundation for further application of electrochemical collision technique in the specific identification of single biological entities.

show abstract

Section: Introductionmentioning

confidence: 99%

Three-Mediator Enhanced Collisions on an Ultramicroelectrode for Selective Identification of Single Saccharomyces cerevisiae

et al. 2022

View full text Add to dashboard Cite

show abstract

PCR virtual temperature sensor design based on system modeling and identification

Wang,

Yang,

Wang

et al. 2025

Measurement

View full text Add to dashboard Cite

A review of Legionella transmission risk in built environments: sources, regulations, sampling, and detection

Yao,

Shen,

Hao

et al. 2024

Front. Public Health

View full text Add to dashboard Cite

The risk of Legionella transmission in built environments remains a significant concern. Legionella can spread within buildings through aerosol transmission, prompting the exploration of airborne transmission pathways and proposing corresponding prevention and control measures based on building characteristics. To this end, a comprehensive literature review on the transmission risk of Legionella in built environments was performed. Four electronic databases (PubMed, Web of Science, Google Scholar, and CNKI) were searched from inception to March 2024 for publications reporting the risk of Legionella transmission in built environments. Relevant articles and gray literature reports were hand-searched, and 96 studies were finally included. Legionella pollution comes from various sources, mainly originates in a variety of built environments in which human beings remain for extended periods. The sources, outbreaks, national standards, regulations, and monitoring techniques for Legionella in buildings are reviewed, in addition to increases in Legionella transmission risk due to poor maintenance of water systems and long-distance transmission events caused by aerosol characteristics. Air and water sampling using various analytical methods helps identify Legionella in the environment, recognize sources in the built environments, and control outbreaks. By comparing the standard regulations of national organizations globally, the authors further highlight gaps and deficiencies in Legionella surveillance in China. Such advancements offer essential insights and references for understanding and addressing Legionella transmission risk in the built environment, with the potential to contribute to safeguarding public health and building environment safety.

show abstract

Chip‐based duplex real‐time PCR for water quality monitoring concerning Legionella pneumophila and Legionella spp.

Cited by 5 publications

References 42 publications

Three-Mediator Enhanced Collisions on an Ultramicroelectrode for Selective Identification of Single Saccharomyces cerevisiae

Three-Mediator Enhanced Collisions on an Ultramicroelectrode for Selective Identification of Single Saccharomyces cerevisiae

PCR virtual temperature sensor design based on system modeling and identification

A review of Legionella transmission risk in built environments: sources, regulations, sampling, and detection

Contact Info

Product

Resources

About