Chip-based drug screening for inhibiting α-glucosidase

Simultaneous ligand fishing and identification of human serum albumin binders from Eucommia ulmoides bark using surface plasmon resonance-high performance liquid chromatography–tandem mass spectrometry

“…Up to now, SPR has been used as a new analytical device to fish out binders of drug targets from pure synthetic or natural molecules and natural product extracts for drug screening, such as protein tyrosine phosphatase 1b (PTP1B) binders [23], ␣-glucosidase binders [24], DNA binders [18], etc. Most ligands bind to HSA in a reversible manner by means of weak chemical bonds such as ionic, van der Waals, hydrogen and hydrophobic bonds with the hydroxyl, carboxyl or other reversible sites available in the amino acids of HSA [25].…”