Chinese yellow wine inhibits production of homocysteine-induced extracellular matrix metalloproteinase-2 in cultured rat vascular smooth muscle cells

Guo, Hangyuan; Wang, Ping; You, Binquan; Xing, Yangbo; Lee, Jong–Dae

doi:10.1016/j.clnu.2007.01.007

Cited by 12 publications

(8 citation statements)

References 41 publications

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“…VitB6, VitB12, and folic acid can reduce HCY [38,39]. These results testified our previous study [14], which found that Chinese yellow wine could inhibit the production of HCY induced extracellular MMP‐2 in cultured rat vascular SMCs. Although plasma hyperhomocysteinemia is an independent risk factor for atherosclerotic disease [40,41], the mechanism is still obscure.…”

Section: Discussionsupporting

confidence: 83%

Chinese Yellow Wine and Red Wine Inhibit Matrix Metalloproteinase‐2 and Improve Atherosclerotic Plaque in LDL Receptor Knockout Mice

Guo

Liu

Shi

et al. 2010

Cardiovascular Therapeutics

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Our previous study found that Chinese yellow wine could inhibit the production of homocysteine (HCY) induced extracellular matrix metalloproteinase‐2 (MMP‐2) in the cultured rat vascular smooth muscle cells. Little is known about the relationship between Chinese yellow wine and atherosclerosis or MMP‐2 in vivo. Thirty‐two LDL Receptor knockout mice on a high‐fat and l‐methionine diet developed plasma hyperhomocysteinemia and atherosclerosis. They were randomly divided into yellow wine group (n = 8), red wine group (n = 8), ethanol group (n = 8), and control group (n = 8), they were sacrificed after 14 weeks. There were no significant differences with plasma total cholesterol (TC), triglyceride (TG), high‐density lipoprotein cholesterol (HDL‐C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels in the four groups. Plasma HCY was significantly decreased in the yellow wine group compared to the other three groups (P < 0.01). Yellow wine and red wine groups significantly reduced the atherosclerosis lesion area compared to ethanol and control groups (P < 0.001). However, there was no significant discrepancy between the yellow wine group and red wine group. Compared to the control group and ethanol group, the production of MMP‐2 reduced 26.8% and 23.6% in the aortic sinus and the activation of MMP‐2 reduced 32.6% and 27.3% in the aortic arch in the yellow wine group; the production of MMP‐2 reduced 25.7% and 22.4% in the aortic sinus and the activation of MMP‐2 reduced 30.2% and 26.6% in the aortic arch in the red wine group. These results suggest that Chinese yellow wine and red wine can inhibit MMP‐2 and improve atherosclerosis, and maybe both Chinese yellow wine and red wine have beneficial effects on cardiovascular disease by inhibiting MMP‐2.

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Section: Discussionsupporting

confidence: 83%

Chinese Yellow Wine and Red Wine Inhibit Matrix Metalloproteinase‐2 and Improve Atherosclerotic Plaque in LDL Receptor Knockout Mice

Guo

Liu

Shi

et al. 2010

Cardiovascular Therapeutics

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“…In the previous studies, we demonstrated the beneficial effect of rice wine on atherosclerosis in in vivo and in vitro experiments. 4,5 However, Chinese rice wine is a complex mixture containing various ingredients such as peptides, oligosaccharides, and polyphenols, and the exact ingredients that have the antiatherosclerosis effect are still unknown. 6 Increased homocysteine (Hcy) level in the blood has been demonstrated as an important risk factor for the development of cardiovascular diseases, and it has already been confirmed that Hcy could promote vascular smooth muscle cell (VSMC) migration and proliferation.…”

Section: Introductionmentioning

confidence: 99%

Polyphenols and Polypeptides in Chinese Rice Wine Inhibit Homocysteine-induced Proliferation and Migration of Vascular Smooth Muscle Cells

Meng

Liu

Zhou

et al. 2016

Journal of Cardiovascular Pharmacology

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The beneficial effect of Chinese rice wine on atherosclerosis has been proved, but the exact components that have the cardiovascular protective effect are still unknown. This study aimed to explore the exact ingredients in Chinese rice wine that could inhibit homocysteine (Hcy)-induced vascular smooth muscle cell (VSMC) proliferation and migration. VSMCs were divided into 7 groups: control, Hcy (1 mmol/L), Hcy + oligosaccharide, Hcy + polypeptides, Hcy + polyphenols, Hcy + alcohol, and Hcy + Chinese rice wine. methyl thiazolyl tetrazolium (MTT) assay, Transwell chambers, and wound-healing assay were used to test the proliferation and migratory ability of the VSMCs. Western blot and gelatin zymography were used to investigate the expressions and activities of metal matrix proteinase 2/9 (MMP-2/9) and tissue inhibitor of metalloproteinase 2 (TIMP-2) in VSMCs. Polypeptides and polyphenols in the Chinese rice wine reduced the proliferation and migration ability of the VSMCs. Furthermore, they also decreased the expression and activity of MMP-2/9 but had no obvious impact on the expression of TIMP-2 in each group. This study further confirms that polypeptides and polyphenols in the Chinese rice wine could inhibit Hcy-induced proliferation and migration of VSMCs and maintain the balance between matrix metalloproteinases (MMPs) and TIMPs.

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“…The detailed Methods section is available as previously described [25,26], or see our Supplemental Material.…”

Section: Methodsmentioning

confidence: 99%

“…Our previous study found that Chinese yellow wine can inhibit MMP-2 and atherosclerosis in the LDL Receptor knockout (LDLR−/-) mice [25]. Indeed, we have previously shown that Chinese yellow wine could inhibit the production of homocysteine (Hcy) induced extracellular MMP-2 in cultured rat vascular smooth muscle cells [26]. Because the VECs is just likely equally important as VSMCs in the development of atherosclerosis, both produce constitutively in vitro MMP-2, the most important enzyme for degradation of ECM components.…”

Section: Identification and Characterization Of Vecsmentioning

confidence: 99%

Chinese Yellow Wine Could Inhibit Production of Matrixmetalloproteinase-2 Induced by Homocysteine in Cultured Rat Vascular Endothelial Cells

Guo¹,

Meng²,

Zhou³

2015

J Nutr Food Sci

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Background: The effects of Chinese yellow wine on the production of homocysteine (Hcy) induced intracellular MMP-2 in cultured rats vascular endothelial cells (VECs) has not been investigated. Methods: Isolation, cultivation, purification and identification of vascular endothelial cells of rat thoracic aorta in vitro were conducted. The VECs in passages 3 to 4 were used in all studies. HCY was used to induce VECs to over expressing MMP-2. Cells were divided into 5 groups: Control, Hcy, Hcy+yellow wine, Hcy+red wine, Hcy+ethanol and the cells were given different treatment for 48 h. The mRNA expression of MMP-2 was detected by FQ-PCR. The western blotting and gelatin zymography were applied to test the protein levels and the enzymatic activity of MMP-2. Results: Hcy could significantly increase the expression and activity of MMP-2 compared with the control group, and could reach the maximum at 500 µ mol/L, cultured for 48 h. Compared with those in Hcy group, the expression and activity of MMP-2 in yellow wine and red wine groups was significantly decreased. No significant difference was shown as between the ethanol group and the Hcy group and no significant discrepancy between the yellow wine and red wine group was found. Conclusions: The result suggest that Hcy promotes the expression and activity of MMP-2, which may play an important role in pathogenesis of atherosclerosis (AS). Treatment with yellow wine or red wine decreases Hcy-induced MMP-2 production in VECs. The attenuation of MMP-2 activation by yellow wine and red wine might contribute to their beneficial effects on the cardiovascular system.

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Chinese yellow wine inhibits production of homocysteine-induced extracellular matrix metalloproteinase-2 in cultured rat vascular smooth muscle cells

Cited by 12 publications

References 41 publications

Chinese Yellow Wine and Red Wine Inhibit Matrix Metalloproteinase‐2 and Improve Atherosclerotic Plaque in LDL Receptor Knockout Mice

Chinese Yellow Wine and Red Wine Inhibit Matrix Metalloproteinase‐2 and Improve Atherosclerotic Plaque in LDL Receptor Knockout Mice

Polyphenols and Polypeptides in Chinese Rice Wine Inhibit Homocysteine-induced Proliferation and Migration of Vascular Smooth Muscle Cells

Chinese Yellow Wine Could Inhibit Production of Matrixmetalloproteinase-2 Induced by Homocysteine in Cultured Rat Vascular Endothelial Cells

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