Chinese yellow cattle PPARA gene: Analyses of expression, polymorphism and trait association

Abstract

Peroxisome proliferator-activated receptor alpha (PPARA) is a member of the nuclear receptor superfamily that regulates key proteins involved in fatty acid oxidation and extracellular lipid metabolism. Given the importance of PPARA in modulating energy metabolism, PPARA may be a suitable candidate gene for assessing economic traits in cattle. In the present study, the genetic diversity of the PPARA gene in Chinese cattle breeds and its effects on growth traits in the Nanyang and Jiaxian breeds were investigated. First, the transcript profiles for PPARA were determined in eight adult cattle tissue types. Next, polymorphisms were identified in the coding and predicted promoter regions of the bovine PPARA gene in 424 animals. Finally, an association study was carried out to evaluate the relationship between PPARA and the development of cattle. Based on quantitative real-time polymerase chain reaction, PPARA was mainly expressed in the kidney and liver. Nine single nucleotide polymorphisms (SNPs) were identified in Chinese domestic cattle, including one novel SNP. Haplotype frequencies and linkage disequilibrium were also investigated. Four SNPs (g.17148558A>T, g.117195348A>G, g.117228160T>C, and g.117233248A>G) showed significant associations with growth traits in NY and JX cattle, including body weight, average daily gain, and hipbone width. These results confirmed the importance of PPARA as a candidate gene for marker-assisted selection for growth traits in cattle.

Effects of Mangifera indica leaves improves blood lipids profile and biochemical indices in high-fat diet-induced hyperlipidaemia rats

“…Results from recent studies have implicated that activation of Peroxisome proliferator-activated receptors associated with the regulation of lipid metabolism and alleviation of inflammation triggers hypolipidemic activities via acting on gene expression involved in lipid homeostasis [19] . There is emerging evidence that PPARα, a positive regulator of lipolysis connected with fatty acid oxidation and extracellular lipid metabolism, has ability to regulate the lipolysis of very low-density lipoproteintriglycerides (VLDL-TG), thereby raising plasma HDL-C levels and decreasing the levels of LDL-C and TG [19,20] . On the other hand, CYP7A1, as a key protein for the control of cholesterol transport in vivo, plays an important role in inhibition cholesterol synthesis and conversion of excess cholesterol to bile acids [21] .…”

Growth retardation-responsive analysis of mRNAs and long noncoding RNAs in the liver tissue of Leiqiong cattle

“…KEGG pathway enrichment analysis showed that PPAR signaling pathway was significantly enriched by the DEGs ACOX2, SCD5, CPT1A, PPARα, ACOX1, RXRB, PCK2, SLC27A5, CPT2, EHHADH and RXRA, playing key roles in the regulation of multiple types of cells metabolism process. As a key member of this pathway, PPARα is involved in lipid metabolism in the liver and muscle, and is a key regulator of hepatocytes growth and metabolism; it has been demonstrated that PPARα could be utilized as a candidate gene for marker-assisted selection for growth in cattle 48 – 50 . CPT1A gene is reported to be involved in regulating the growth traits in goat 51 .…”

Insertion of 643bp Retrotransposon Upstream of PPARγ CDS Is Associated with Backfat of Large White Pigs

“…Single nucleotide polymorphisms (SNPs) in PPARs genes have been linked to growth, development, and meat quality in various species such as cattle and sheep [15][16][17]. A 636A>G SNP in the PPARα gene influenced adipose accumulation in Polish landrace pigs [18].…”