Chinese patent medicine Xin-Ke-Shu inhibits Ca 2+ overload and dysfunction of fatty acid β -oxidation in rats with myocardial infarction induced by LAD ligation

Yong, Yang; Jia, Hongmei; Meng, Yijun; Zhou, Chao; Sun, Lili; Zhao, Yang; Zhang, Hongwu; Zou, Zhong‐Mei

doi:10.1016/j.jchromb.2018.01.038

Cited by 17 publications

(7 citation statements)

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“…The proteomic analysis revealed protein alterations in NIH3T3 cells under OGD conditions, in which the upregulated differentially expressed proteins were involved in fatty acid degradation. Importantly, proteins related to the fatty acid degradation pathway in the heart have indeed been reported to be highly expressed after MI, , which is consistent with the results of this study. A KEGG pathway analysis of the differentially expressed proteins affected by TSA did not show an enrichment of the fatty acid metabolism pathway in NIH3T3 cells under OGD conditions.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis Reveals the Effect of Trichostatin A and Bone Marrow-Derived Dendritic Cells on the Fatty Acid Metabolism of NIH3T3 Cells under Oxygen–Glucose Deprivation Conditions

et al. 2020

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Fibroblasts mediate acute wound healing and long-term tissue remodeling with scarring after tissue injury. Following myocardial infarction (MI), necrotized cardiomyocytes become replaced by secreted extracellular matrix proteins produced by fibroblasts. Dendritic cells (DCs) can migrate from the bone marrow to the infarct areas and infarct border areas to mediate collagen accumulation after MI. Trichostatin A (TSA) is known to regulate apoptosis and proliferation in fibroblasts and affect the functions of DCs under oxygen−glucose deprivation (OGD) conditions. In this study, we used label-free quantitative proteomics to investigate the effects of TSA and bone marrow-derived dendritic cells (BMDCs) on NIH3T3 fibroblasts under OGD conditions. The results showed that the fatty acid degradation pathway was significantly upregulated in NIH3T3 cells under OGD conditions and that the fatty acid synthesis pathway was significantly downregulated in NIH3T3 cells treated with conditioned media (CM) from BMDCs treated with TSA under OGD conditions [BMDCs−CM(TSA)]. In addition, BMDCs−CM(TSA) significantly decreased the levels of triglycerides and free fatty acids and mediated fatty acid metabolism-related proteins in NIH3T3 cells under OGD conditions. In summary, this proteomics analysis showed that TSA and BMDCs affect fatty acid metabolism in NIH3T3 cells under OGD conditions.

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Section: Discussionmentioning

confidence: 99%

Proteomic Analysis Reveals the Effect of Trichostatin A and Bone Marrow-Derived Dendritic Cells on the Fatty Acid Metabolism of NIH3T3 Cells under Oxygen–Glucose Deprivation Conditions

et al. 2020

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“…Coronary heart disease includes a series of clinical conditions, such as myocardial infarction and arrhythmia, caused by atherosclerosis, which leads to narrowing of the coronary artery lumen and insufficient blood supply to the heart muscle (Lei et al, 2018;Liu et al, 2018). Coronary heart disease is the main cause of sudden cardiac death worldwide, accompanied by apoptosis and myocardial fibrosis, leading to HF (Yang et al, 2018a;Yang et al, 2018b).…”

Section: Coronary Heart Disease and Myocardial Infarctionmentioning

confidence: 99%

“…Xin-Ke-Shu (XKS), Guanxinshutong capsule (GXSTC), modified Yiqi decoction (MYQ), and other Chinese herbal compound preparations are involved in the regulation of calcium signaling during the treatment of coronary heart disease and myocardial infarction (Yang et al, 2018a;Liu et al, 2017b;Yu et al, 2017) (Table 5). Xin-Ke-Shu tablet (XKS) is mainly composed of Salvia miltiorrhiza, Radix Puerariae, Hawthorn, Panax Notoginseng and Radix Aucklandiae.…”

Section: Coronary Heart Disease and Myocardial Infarctionmentioning

confidence: 99%

“…XKS improved LAD-induced myocardial infarction in rats, which was attributed to its effect on inhibition of the overexpression of CaMKII, thereby alleviating Ca 2+ overload. Moreover, XKS also regulates lysophosphatidylcholine and long-chain fatty acid expression and maintains cell membrane stability (Yang et al, 2018a). GXSTC is commonly used in the clinical treatment of coronary heart disease, angina pectoris, and other CVDs.…”

Section: Coronary Heart Disease and Myocardial Infarctionmentioning

confidence: 99%

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Therapeutic Effects of Traditional Chinese Medicine on Cardiovascular Diseases: the Central Role of Calcium Signaling

Zhang

et al. 2021

Front. Pharmacol.

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Calcium, as a second messenger, plays an important role in the pathogenesis of cardiovascular diseases (CVDs). The malfunction of calcium signaling in endothelial cells and vascular smooth muscle cells promotes hypertension. In cardiomyocytes, calcium overload induces apoptosis, leading to myocardial infarction and arrhythmias. Moreover, the calcium–calcineurin–nuclear factor of activated T cells (NFAT) pathway is essential for expressing the cardiac pro-hypertrophic gene. Heart failure is also characterized by reduced calcium transient amplitude and enhanced sarcoplasmic reticulum (SR) calcium leakage. Traditional Chinese medicine (TCM) has been used to treat CVDs for thousands of years in China. Because of its multicomponent and multitarget characteristics, TCM's unique advantages in CVD treatment are closely related to the modulation of multiple calcium handling proteins and calcium signaling pathways in different types of cells involved in distinct CVDs. Thus, we systematically review the diverse mechanisms of TCM in regulating calcium pathways to treat various types of CVDs, ranging from hypertrophic cardiomyopathy to diabetic heart disease.

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“…Maesen & S.M.Almeida ex Sanjappa & Predeep (Gegen in Chinese), Crataegus pinnatifida Bunge (Shanzha in Chinese), Panax notoginseng (Burkill) F.H.Chen (Sanqi in Chinese), and Aucklandia costus Falc (Muxiang in Chinese), in a ratio of 15:15:15:1:1, have been extensively used for the treatment of cardiovascular diseases (CVDs) in China for decades ( Peng et al, 2011 ). Pharmacological studies have revealed that XKST can effectively regulate the abnormal changes in blood lipid and lipid peroxide and have a protective effect on myocardial ischemia and reperfusion injury in animal models ( Liu et al, 2014a ; Liu et al, 2014b ; Yang et al, 2018 ). However, up to now, the quality-control index of XKST is only limited to a few index ingredients in Chinese Pharmacopoeia, which cannot reflect the characteristics of overall pharmacological effects through multi-components of TCM prescription, and there is only little literature available for the holistic quality identification of XKST ( Peng et al, 2011 ; Wang et al, 2012 ; Qing et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

An Integrated Strategy to Identify and Quantify the Quality Markers of Xinkeshu Tablets Based on Spectrum-Effect Relationship, Network Pharmacology, Plasma Pharmacochemistry, and Pharmacodynamics of Zebrafish

et al. 2022

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Xinkeshu tablets (XKST), a traditional Chinese patent medicine (CPM), have served in the clinical treatment of cardiovascular diseases (CVDs) for decades. However, its pharmacodyamic material basis was still unclear, and the holistic quality control has not been well established due to the lack of systematic research on the quality markers. In this experiment, the heart rate recovery rate of a zebrafish larva was used to evaluate the traditional pharmacological effect of XKST i.e., antiarrhythmic effect. The HPLC fingerprints of 16 batches of XKST samples were obtained, and antiarrhythmic components of XKST were identified by establishing the spectrum-effect relationship between HPLC fingerprints and heart rate recovery rate of zebrafish larva with orthogonal signal correction and partial least squares regression (OSC–PLSR) analysis. The anticardiovascular disease components of XKST were identified by mapping the targets related to CVDs in network pharmacology. The compounds of XKST absorbed and exposed in vivo were identified by ultra-high performance liquid chromatography Q-Exactive high-resolution mass spectrometry (UHPLC-Q-Exactive HRMS). Based on the earlier studies, combined with five principles for identifying quality markers and verified by a zebrafish arrhythmia model, danshensu, salvianolic acid A, salvianolic acid B, daidzein, and puerarin were identified as quality markers of XKST. In total, 16 batches of XKST samples were further quantified with the method established in this study. Our study laid the foundation for the quality control of XKST. The integrated strategy used in the study of XKST could be applied for the identification and quantification of quality markers of other CPMs as well.

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Chinese patent medicine Xin-Ke-Shu inhibits Ca 2+ overload and dysfunction of fatty acid β -oxidation in rats with myocardial infarction induced by LAD ligation

Cited by 17 publications

References 54 publications

Proteomic Analysis Reveals the Effect of Trichostatin A and Bone Marrow-Derived Dendritic Cells on the Fatty Acid Metabolism of NIH3T3 Cells under Oxygen–Glucose Deprivation Conditions

Proteomic Analysis Reveals the Effect of Trichostatin A and Bone Marrow-Derived Dendritic Cells on the Fatty Acid Metabolism of NIH3T3 Cells under Oxygen–Glucose Deprivation Conditions

Therapeutic Effects of Traditional Chinese Medicine on Cardiovascular Diseases: the Central Role of Calcium Signaling

An Integrated Strategy to Identify and Quantify the Quality Markers of Xinkeshu Tablets Based on Spectrum-Effect Relationship, Network Pharmacology, Plasma Pharmacochemistry, and Pharmacodynamics of Zebrafish

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