Chinese Medicine Formula Lingguizhugan Decoction Improves Beta-Oxidation and Metabolism of Fatty Acid in High-Fat-Diet-Induced Rat Model of Fatty Liver Disease

Liu, Tao; Yang, Lili; Zou, Lu; Li, Dongfei; Wen, Hong-Zhu; Zheng, Peiyong; Xing, Lian-Jun; Song, Haiyan; Tang, Xudong; Ji, Guang

doi:10.1155/2013/429738

Cited by 28 publications

(39 citation statements)

References 23 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mice that lack PPARα display attenuated fatty acid β-oxidation, resulting in increased free fatty acids, as well as TG accumulation that causes fatty liver [26]. In the process of fatty acid β-oxidation, ACSL-1 catalyses long-chain fatty acids to long-chain fatty acyl-CoA and plays an important role in channelling fatty acids either towards complex lipid synthesis and storage or towards oxidation [27]. …”

Section: Discussionmentioning

confidence: 99%

Acetic Acid Influences BRL-3A Cell Lipid Metabolism via the AMPK Signalling Pathway

Xiao

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Acetic acid (AcOH), a short-chain fatty acid, is reported to have some beneficial effects on metabolism. Therefore, the aim of this study was to investigate the regulatory mechanism of acetic acid on hepatic lipid metabolism in BRL-3A cells. Methods: We cultured and treated BRL-3A cells with different concentrations of sodium acetate (neutralized acetic acid) and BML-275 (an AMPKα inhibitor). The total lipid droplet area was measured by oil red O staining, and the triglyceride content was determined by a triglyceride detection kit. We detected mRNA and protein levels of lipid metabolism-related signalling molecules by RT-PCR and Western blot. Results: Acetic acid treatment increased AMPKα phosphorylation, which subsequently increased the expression and transcriptional activity of peroxisome proliferator-activated receptor α and upregulated the expression of lipid oxidation genes. These changes ultimate led to increasing levels of lipid oxidation in BRL-3A cells. Furthermore, elevated AMPKα phosphorylation reduced the expression and transcriptional activity of the sterol regulatory element-binding protein 1c, which reduced the expression of lipogenic genes, thereby decreasing lipid biosynthesis in BRL-3A cells. Consequently, triglyceride content in acetate-treated BRL-3A cells was significantly decreased. Conclusions: These results indicate that acetic acid activates the AMPKα signalling pathway, leading to increased lipid oxidation and decreased lipid synthesis in BRL-3A cells, thereby reducing liver fat accumulation in vitro.

show abstract

Section: Discussionmentioning

confidence: 99%

Acetic Acid Influences BRL-3A Cell Lipid Metabolism via the AMPK Signalling Pathway

Xiao

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…During the β-oxidation of a fatty acid, TG is hydrolyzed into FFA and glycerine by lipase, and FFA generates energy by β-oxidation, however, the fatty acid catabolism process is completed by ACSL-1 [20]. During this process, the fatty acid enters the mitochondria.…”

Section: Discussionmentioning

confidence: 99%

SREBP-1c Gene Silencing can Decrease Lipid Deposits in Bovine Hepatocytes Cultured <b><i>in Vitro</i></b>

Deng

Yin

et al. 2014

Cell Physiol Biochem

View full text Add to dashboard Cite

Background: Fatty liver is a major metabolic disorder that occurs during early lactation in high-producing dairy cows. Sterol regulatory element-binding protein-1c (SREBP-1c) is an important transcription factor that regulates lipid synthesis by regulating the expression of lipid metabolism genes. Methods: In this study, we reduced the expression of SREBP-1c by adenovirus-mediated SREBP-1c with a low expression vector (AD-GFP-SREBP-1c) to study the effects of SREBP-1c on lipid deposits in bovine hepatocytes. The expression levels and enzyme activities of SERBP-1c and its target genes were determined by real-time PCR, western blot, and ELISA. Results: These results showed that Ad-GFP-SREBP-1c could inhibit SREBP-1c expression. The expression of the lipid synthesis enzyme acetyl-CoA carboxylase (ACC) was down-regulated. The expression levels of the lipid oxidation enzymes long-chain fatty acyl-COA synthetase (ACSL-1), carnitine palmitoyltransferase І (CPT-І), carnitine palmitoyltransferase II (CPT- II), and β-hydroxyacyl-CoA-DH (HADH) were significantly elevated. Furthermore, the expression levels of factors involved in the assembly and transport of very low-density lipoproteins (VLDLs), such as apolipoprotein B100 (ApoB), apolipoprotein E (ApoE), and microsomal triglyceride transfer protein (MTTP) were decreased comparison with the negative control and the blank control groups, but the low-density lipoprotein receptor (LDLR) was elevated. The concentrations of TG (triglyceride) and VLDL were also reduced. Conclusion: These data suggest that low SREBP-1c expression can decrease lipid synthesis, increase lipid oxidation, and decrease the TG and VLDL content in bovine hepatocytes.

show abstract

“…According to the dose-equivalence equation between rats and humans (9 g/d) [8], the dose of Mangxiao and Dahuang for rats was 1.5 g/kg, and the dose of Mangxiao for mice was 1.0 g/kg. Mangxiao or Dahuang decoction was prepared in accordance with conventional TCM decocting methods, respectively [9, 10]. Quality was controlled with high-performance liquid chromatography (HPLC) and ion chromatography [11, 12].…”

Section: Methodsmentioning

confidence: 99%

Specific Link between Lung and Large Intestine: A New Perspective on Neuropeptide Secretion in Lung with Herbal Laxative Stimulation

Zhong

Zheng

et al. 2013

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Background. To investigate the specific link between lung and large intestine. Methods. Rat COPD-like model was prepared. Mirabilite or Chinese rhubarb was administrated intragastrically to stimulate the large intestine. Histological analysis of lung inflammation was assessed. The tissues levels of SP, VIP, NK1R, VIPR1, and VIPR2 were measured by using ELISA kits. In addition, mouse model of allergic asthma was prepared. Mirabilite was administrated intragastrically to stimulate the large intestine. Airway responsiveness and lung inflammation were assessed. The tissues levels of SP, VIP, NKA, NKB, NK1R, VIPR1, and VIPR2 were measured by using ELISA kits. Results. Stimulating the intestine with Mangxiao or Dahuang, SP, NK-1R, VIP, VIPR1, and VIPR2 were significantly increased in intestine tissues of rats with COPD and mice with asthma. Meanwhile, the SP and NK1R were significantly decreased, while VIP, VIPR1, and VIPR2 were significantly increased in lung tissues. An abnormal secretion of SP and VIP can be observed in other tissues; however, no marked changes were found in the receptors. The NKA and NKB levels were similar in lung tissues of mice with asthma among groups. Conclusions. Stimulating intestine with Mangxiao or Dahuang can specifically regulate the secretion of SP, VIP, and the receptors in lung tissues.

show abstract

Chinese Medicine Formula Lingguizhugan Decoction Improves Beta-Oxidation and Metabolism of Fatty Acid in High-Fat-Diet-Induced Rat Model of Fatty Liver Disease

Cited by 28 publications

References 23 publications

Acetic Acid Influences BRL-3A Cell Lipid Metabolism via the AMPK Signalling Pathway

Acetic Acid Influences BRL-3A Cell Lipid Metabolism via the AMPK Signalling Pathway

SREBP-1c Gene Silencing can Decrease Lipid Deposits in Bovine Hepatocytes Cultured <b><i>in Vitro</i></b>

Specific Link between Lung and Large Intestine: A New Perspective on Neuropeptide Secretion in Lung with Herbal Laxative Stimulation

Contact Info

Product

Resources

About