Chinese herbal medicines mixture improved antioxidant enzymes, immunity and disease resistance to infectious hematopoietic necrosis virus infection in rainbow trout (Oncorhynchus mykiss)

Wang, Qi; Huang, Jinqiang; Li, Yongjuan; Wu, Shenji; Zhao, Lu; Pan, Yucai; Kang, Yujun; Liu, Zhe

doi:10.1007/s10499-023-01319-w

Cited by 1 publication

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“…The 10 kinds of CHM used in this experiment ( Table 1 ), all purchased from a local drugstore, were finely ground by using a 100-mesh sieve, and each CHM was uniformly mixed well according to the same mass ratio, dried under sterile control, and then precipitated and stored at 4°C until use. The composition of the feed of rainbow trout is based on the observations from the previous study ( 28 ). CHMM was added at a ratio of 0, 10, 20, and 30 g/kg.…”

Section: Methodsmentioning

confidence: 99%

Dietary supplementation of Chinese herbal medicines enhances the immune response and resistance of rainbow trout (Oncorhynchus mykiss) to infectious hematopoietic necrosis virus

Wang,

Pan,

Huang

et al. 2024

Front. Vet. Sci.

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Rainbow trout is a widely farmed economical cold-water fish worldwide, but the prevalence of infectious hematopoietic necrosis virus (IHNV) presents a severe risk to the aquaculture industry, resulting in high mortality and huge economic losses. In this study, the impacts of different concentrations (0, 10, 20, and 30 g/kg) of Chinese herbal medicine mixture (CHMM) on the immune response and resistance of rainbow trout to IHNV infection were evaluated. The results show that CHMM noticeably increased (P < 0.05) T-SOD, CAT, AST, ALT, ACP, and AKP activities and decreased MDA content. NF-κB, TNF-α, IFN-β, IL-1β, JAK1, HSP70, and HSP90 expressions were significantly upregulated (P < 0.05) in all CHMMs, while SOCS2 expression was downregulated (P < 0.05). Following infection with IHNV, feeding rainbow trout with varying amounts of CHMM resulted in noticeably increased (P < 0.05) T-SOD, ACP, and AKP activities and significantly decreased (P < 0.05) MDA content and AST and ALT activities. TNF-α, IFN-β, IL-1β, HSP70, and HSP90 expressions were significantly upregulated (P < 0.05) in all CHMMs, while the expressions of JAK1 and SOCS2 were downregulated. The expression level of the IHNV G protein gene at a dosage of 20 g/kg was notably lower than that of the other CHMM feeding groups. This study provides a solid scientific basis for promoting CHMM as an immunostimulant for boosting antiviral immunity in rainbow trout.

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Section: Methodsmentioning

confidence: 99%