Chinese herbal medicine promote tissue differentiation in colorectal cancer by activating HSD11B2

Lü, Qing; Zhang, Ying; Song, Fuyao; Ding, Yanqing

doi:10.1016/j.abb.2020.108644

Cited by 13 publications

(8 citation statements)

References 46 publications

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“…The Results of Ming-Zhi Zhang et al demonstrated that inhibition of HSD11B2 selectively blocked the COX-2 pathway in tumors and inhibited the development of colon cancer in humans [23]. At present, some studies have proved that HSD11B2 is closely related to the proliferation and metastasis of cancer [21,23,24]. Combined with the above results, we inferred that HSD11B2 expression is associated with proliferation and metastasis of renal clear cell carcinoma.In addition, GSEA analysis showed that low expression of HSD11B2 is involved in tumor-related pathways and may promote tumor metastasis.…”

Section: Discussionsupporting

confidence: 63%

Identification and validation of HSD11B2 as a biomarker for metastasis and prognosis of clear cell renal cell carcinoma

Miao

Song

Teng

et al. 2021

Preprint

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Background : Metastasis of clear cell renal cell carcinoma (ccRCC) is an important cause of death. The purpose of this study was to study the key gene in the process of tumor metastasis of ccRCC. Methods : Expression profiles of metastatic ccRCC and primary ccRCC were downloaded from the GEO database. Expression profiling and clinical data of ccRCC were downloaded from The Cancer Genome Atlas (TCGA) dataset. The R limma package was used to identify differentially expressed genes (DEGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were conducted for overlapping DEGs. Verification of mRNA expression and survival analysis in the GEPIA2 database further identified the key gene. The expression of the key gene in clinical specimens was detected by quantitative real-time PCR (qRT-PCR). Univariate and multivariate cox analysis were performed to determine whether the key gene was independent prognostic factor. Gene Set Enrichment Analysis (GSEA) was used to identify HSD11B2 related signaling pathways. The correlations between the key gene and tumor immune infiltrates were investigated via TISIDB database. Results ：A total of 20 DEGs were screened from GSE22541, GSE85258, and GSE105261 datasets. Enrichment analysis indicated that the DEGs were mainly enriched in the extracellular matrix organization, collagen-containing extracellular matrix, extracellular matrix structural constituent, and protein digestion and absorption. Verification of mRN expression and survival analysis identified the key gene HSD11B2. qRT-PCR results showed A that HSD11B2 level was significantly down-regulated in ccRCC tissues compared with adjacent normal tissues. Multivariate Cox regression analysis showed that HSD11B2 expression was an independent prognostic factor for ccRCC patients. GSEA enrichment results showed that low expression of HSD11B2 could enrich cancer signaling pathways such as Nod-like receptor signaling pathway, cytoplasmic DNA sensing pathway and P53 signaling pathway. Immune analysis showed a significant correlation between HSD11B2 and tumor immune infiltrates in ccRCC. Conclusions : These findings suggest that HSD11B2 may play a key role in the metastasis of ccRCC, and HSD11B2 is correlated with prognosis and tumor immune infiltrates.

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Section: Discussionsupporting

confidence: 63%

Identification and validation of HSD11B2 as a biomarker for metastasis and prognosis of clear cell renal cell carcinoma

Miao

Song

Teng

et al. 2021

Preprint

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“…After 48 h of treatment, the IC 50 values of Sanguisorba officinalis L. in RKO-P/R and HCT15-P/R were 148.9 µg/mL, 121.7 µg/mL, 163.6 µg/mL, and 105.2 µg/mL, respectively. Qi found Sanguisorba officinalis L. had an activating effect on the key target proteins corticosteroid 11-beta-dehydrogenase isozyme 2, the enzyme encoded by HSD11B2, which was markedly decreased in colorectal cancer tissues and was positively correlated with the overall survival time of patients [21]. The results indicated Sanguisorba officinalis L. could promote tissue differentiation in colorectal cancer.…”

Section: Discussionmentioning

confidence: 88%

A Bioactive Compound from Sanguisorba officinalis L. Inhibits Cell Proliferation and Induces Cell Death in 5-Fluorouracil-Sensitive/Resistant Colorectal Cancer Cells

et al. 2021

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Colorectal cancer (CRC) is one of the most common cancer in the world. The first line chemotherapeutic agent, 5-fluorouracil (5-FU), plays a predominant role in the clinical treatment of CRC. However, with the wide use of 5-FU, more and more CRC patients have been obtaining drug resistance to 5-FU, which leads to a large amount of treatment failures. One of the effective strategies to overcome this obstacle is to find bioactive natural products from traditional medicine. In our previous work, Sanguisorba officinalis L. was found to exert a strong anti-proliferative activity against 5-FU-senstive/resistant CRC cells. Therefore, several compounds were isolated from this herb and screened for their anti-CRC effects to find promising compounds. Among them, a triterpenoid compound named 3β-[(α-l-arabinopyranosyl) oxy]-urs-12,18(19)-dien-28-oic acid β-d-glucopyranosyl ester (AGE), showed strong activity against both 5-FU-senstive and resistant CRC cells. In order to further study the mechanism of AGE on CRC cells, flow cytometer analysis, mitochondrial membrane potential (MMP) measurement, Western blotting, and RT-PCR assays were performed. Results demonstrated that AGE induced cell death by apoptosis pathway and autophagy, and inhibited cell proliferation via cell cycle arrest in G0-G1 phase mediated by Wnt signaling pathway. Therefore, AGE may be a potential bioactive compound for CRC treatment in clinic.

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“…The occurrence of liver cancer is mostly attributable to infection with hepatitis B virus, liver cirrhosis, alcoholism, smoking, and other detrimental habits (28). Studies have shown that Asian traditional herbal medicine exerts antiinflammatory (29,30), antibacterial (31), antitumor (32)(33)(34), and other physiological actions (35)(36)(37). Its antitumor effect is relatively significant.…”

Section: Discussionmentioning

confidence: 99%

Anticancer Effect of ERM210 on Liver Cancer Cells Through ROS/Mitochondria-dependent Apoptosis Signaling Pathways

Lee¹,

Gong

Xie

et al. 2021

In Vivo

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Background/Aim: Asian Traditional medicines are renowned for their antitumor properties and are efficacious in the clinical treatment of various cancer types. ERM210 is a Korean traditional medicine comprising nine types of medicinal plants. In the present study, we examined the pro-apoptotic effect and molecular mechanisms of the effects of ERM210 on HepG2 liver cancer cells. Materials and Methods: The cytotoxicity of ERM210 on HepG2 cells was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide and wound-healing assays, and apoptosis and signaling pathways by fluorescence microscopy flow cytometry and western blotting. Results: ERM210 significantly impaired HepG2 cell viability and enhanced mitochondria-dependent cellular apoptosis in a time-and dose-dependent manner by up-regulating the expression of caspases 3, 7 and 9, and of BCL2 apoptosis regulator (BCL2)-associated X, apoptosis regulator (BAX) proteins, whilst down-regulating that of BCL2 protein. Furthermore, ERM210 treatment increased accumulation of cellular and mitochondrial reactive oxygen species (ROS) and significantly inhibited cell migration. Additionally, all these phenomena were reversed by treating with the ROS scavenger N-acetylcysteine. The analysis of signaling proteins revealed that ERM210 significantly up-regulated the phosphorylation of ROS-dependent mitogen-activated protein kinases (p38, extracellular-regulated kinase, and c-Jun Nterminal kinase in HepG2 liver cancer cells. Conclusion: ERM210 exerts anticancer effects in HepG2 liver cancer cells by up-regulating ROS/mitochondria-dependent apoptosis signaling, providing new insight into the possibility of employing this traditional medicine for the clinical treatment of liver cancer.A wide variety of unique traditional herbal medicines abound in Asia. These medicines have numerous advantages over conventional chemotherapy drugs, such as low toxicity, easy absorption, and multi-target action (1, 2). According to the World Health Organization, traditional medicine is "used in the maintenance of health as well as in the prevention, diagnosis, improvement or treatment of physical and mental illness" (3). This underlies the long-established traditional use of herbal-based medicines. It includes herbal medicines and herbal finished products, which contain plants and other combination as active ingredients (4). In recent years, numerous studies have shown that herbal medicine is effective in treating insomnia (3), Alzheimer's disease (5), cardiovascular diseases (6), polycystic ovary syndrome (7), esophageal cancer (8), and other diseases (9, 10). Herbal medicines contain abundant anticancer compounds that directly affect cancer cells and have been utilized as anticancer agents far longer than any of the chemotherapy drugs.Cancer is an intractable human disease. Liver cancer is for the third leading cause of mortality (11). Hepatocellular carcinoma constitutes the primary and most common liver cancer subtype, with contributing risk factors including 2599 This article ...

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Chinese herbal medicine promote tissue differentiation in colorectal cancer by activating HSD11B2

Cited by 13 publications

References 46 publications

Identification and validation of HSD11B2 as a biomarker for metastasis and prognosis of clear cell renal cell carcinoma

Identification and validation of HSD11B2 as a biomarker for metastasis and prognosis of clear cell renal cell carcinoma

A Bioactive Compound from Sanguisorba officinalis L. Inhibits Cell Proliferation and Induces Cell Death in 5-Fluorouracil-Sensitive/Resistant Colorectal Cancer Cells

Anticancer Effect of ERM210 on Liver Cancer Cells Through ROS/Mitochondria-dependent Apoptosis Signaling Pathways

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