Chinese Herbal Formula, Modified Danggui Buxue Tang, Attenuates Apoptosis of Hematopoietic Stem Cells in Immune-Mediated Aplastic Anemia Mouse Model

Zhou, Jingwei; Li, Xue; Deng, Peiying; Wei, Yi; Liu, Juan; Meng, Chen; Xu, Yunling; Zhang, Dongmei; Zhu, Li; Lou, Lixia; Dong, Bin; Jin, Qiushuo; Chai, Limin

doi:10.1155/2017/9786972

Cited by 20 publications

(15 citation statements)

References 31 publications

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“…The constituents of Coptis chinensis were analyzed by HPLC-ESI/MS n . The specific measurement procedures referred to our previous works methods as previously described [ 12 – 14 ]. Peak View Software 2.2 was used to analyze the data, including retention time, accurate mass and MS/MS spectrum comparison.…”

Section: Methodsmentioning

confidence: 99%

Coptidis alkaloids extracted from Coptis chinensis Franch attenuate IFN-γ-induced destruction of bone marrow cells

Meng

Wang

et al. 2020

PLoS ONE

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Coptidis alkaloids are the primary active components of Coptis chinensis Franch. Clinical and pharmacodynamic studies have confirmed that Coptidis alkaloids have multiple therapeutic effects including anti-inflammatory, antioxidant and antitumor effects, and they are usually used to treat various inflammatory disorders and related diseases. Mouse bone marrow cells (BMCs) were isolated from BALB/c mice. Immune-mediated destruction of BMCs was induced by interferon (IFN)-γ. High-performance liquid chromatography-electrospray ionization/ mass spectrometry was used to analyze the ingredients of the aqueous extract from Coptis chinensis Franch. The results confirmed that Coptidis alkaloids were the predominant ingredients in the aqueous extract from Coptis chinensis. The functional mechanism of Coptidis alkaloids in inhibiting immune-mediated destruction of BMCs was studied in vitro. After Coptidis alkaloid treatment, the percentages of apoptotic BMCs and the proliferation and differentiation of helper T (Th) cells and regulatory T (Treg) cells were measured by flow cytometry. The expression and distribution of T-bet in BMCs were observed by immunofluorescence. Western blotting analysis was used to assay the expression of key molecules in the Fas apoptosis and Jak/Stats signaling pathways in BMCs. We identified five alkaloids in the aqueous extract of Coptis chinensis. The apoptotic ratios of BMCs induced by IFN-γ were decreased significantly after Coptidis alkaloid treatment. The levels of key molecules (Fas, Caspase-3, cleaved Caspase-3, Caspase-8 and Caspase-8) in Fas apoptosis signaling pathways also decreased significantly after treatment with low concentrations of Coptidis alkaloids. Coptidis alkaloids were also found to inhibit the proliferation of Th1 and Th17 cells and induce the differentiation of Th2 and Treg cells; further, the distribution of T-bet in BMCs was decreased significantly. In addition, the levels of Stat-1, phospho-Stat-1 and phospho-Stat-3 were also reduced after Coptidis alkaloid treatment. These results indicate that Coptidis alkaloids extracted by water decoction from Coptis chinensis Franch could inhibit the proliferation and differentiation of T lymphocytes, attenuate the apoptosis of BMCs, and suppress the immunemediated destruction of the BMCs induced by pro-inflammatory cytokines.

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Section: Methodsmentioning

confidence: 99%

Coptidis alkaloids extracted from Coptis chinensis Franch attenuate IFN-γ-induced destruction of bone marrow cells

Meng

Wang

et al. 2020

PLoS ONE

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“…EMSA eritin, a polyherbal formulation had increased proliferation of HSC in irradiated BALB/c mice in vivo and triggered differentiation into the lymphopoiesis lineages [40]. Inducing of proliferation and attenuating of apoptosis were observed when an immune-mediated aplastic anaemia mouse model was treated with a modified Chinese herbal formula prepared with Radix astragali, Radix Angelicae sinensis and Coptis chinensis Franch [41].…”

Section: Effects Of Herbal Extracts On Human Stem Cells 21 Stimulatmentioning

confidence: 99%

Application of Herbal Medicine as Proliferation and Differentiation Effectors of Human Stem Cells

Udagama¹,

Udalamaththa²

2019

Herbal Medicine

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One of the main streams of traditional medicine is herbal medicine; a wide range of medicinal plants and their individual parts are used for therapy. Though not scientifically validated, this traditional medicine practice is much popular in countries such as India, China and Sri Lanka and in many other countries in South, Southeast and Eastern Asia due mainly to its healing capabilities. More recently, scientists initiated the chemical analyses of these medicinal plants, obtaining invaluable results. The latest addition to such investigations is studies on effects of herbal extracts on different types of stem cells. An extensive summary of such reported studies is presented in this chapter, mainly categorizing these into proliferation stimulatory effects on stem cells and inhibitory effects on cancer stem cells (CSCs), where both properties are beneficial in cell therapy procedures. At present, standardizing the products and limited knowledge on the mechanisms of action and pathways of these have critically limited the use of herbal extracts in therapeutics. However, we believe that in the near future scientists would be focusing on herbal remedies to replace the use of synthetic stimulants and cancer drugs to overcome the disadvantages of these, such as toxicity, side effects and exorbitant costs.

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“…Our previous studies have verified the hematopoietic function of modified DBT in anaplastic anemia mouse model by bone marrow suppression. The results indicated that modified DBT could decrease the proliferation and differentiation of effector T cells, impair Treg-mediated immunosuppressive functions, attenuate immune-mediated destruction of HSCs, repair hematopoietic failure and recover the hematopoietic function of HSCs in vivo [18,19].…”

Section: Introductionmentioning

confidence: 99%

The combination of Radix Astragali and Radix Angelicae Sinensis attenuates the IFN-γ-induced immune destruction of hematopoiesis in bone marrow cells

Liu

Wei

Wang

et al. 2019

BMC Complement Altern Med

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Background: Radix Astragali and Radix Angelicae Sinensis are two herbs that compose Danggui Buxue Tang (an herbal formula for treatment of anemia diseases). In this study, we explored the molecular mechanism and effective targets to immune destruction of bone marrow (BM) cells treated with Radix Astragali, Radix Angelicae Sinensis or a combination of two agents. The potential synergic advantages of two herbs should also be explored. Methods: The constituents of Radix Astragali and Radix Angelicae Sinensis were analyzed by high performance liquid chromatography-electrospray ionization/mass spectrometer system BM cells were separated from limbs of BALB/c mice, and immune destruction was induced with IFN-γ. The percentages of hematopoietic stem cells (HSCs) and CD3 + T cells were detected by flow cytometry. The distribution of T-bet and changes in the combination of SAP and SLAM in BM cells were observed by immunofluorescence. Western blotting was used to assay the expression of key molecules of the eIF2 signaling pathway in BM cells. Results: Seven constituents of Radix Astragali and six constituents of Radix Angelicae Sinensis were identified. The percentages of HSCs increased significantly after treatment with Radix Angelicae Sinensis, especially at high concentrations. The percentages of CD3 + T cells were significantly decreased after Radix Astragali and Radix Angelicae Sinensis treatment. However, the synergistic function of two-herb combinations was superior to that of the individual herbs alone. The distribution of T-bet in BM cells was decreased significantly after Radix Angelicae Sinensis treatment. The number of SLAM/SAP double-stained cells was increased significantly after Radix Astragali treatment at low concentrations. The phosphorylation levels of eIF2α were also reduced after Radix Astragali and Radix Angelicae Sinensis treatment. Conclusions: Radix Astragali and Radix Angelicae Sinensis could intervene in the immunologic balance of T lymphocytes, inhibit the apoptosis of BM cells induced by immune attack, restore the balance of the T cell immune response network and recover the hematopoietic function of HSCs. The synergistic effects of Radix Astragali and Radix Angelicae Sinensis were superior to those of each herb alone.

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Chinese Herbal Formula, Modified Danggui Buxue Tang, Attenuates Apoptosis of Hematopoietic Stem Cells in Immune-Mediated Aplastic Anemia Mouse Model

Cited by 20 publications

References 31 publications

Coptidis alkaloids extracted from Coptis chinensis Franch attenuate IFN-γ-induced destruction of bone marrow cells

Coptidis alkaloids extracted from Coptis chinensis Franch attenuate IFN-γ-induced destruction of bone marrow cells

Application of Herbal Medicine as Proliferation and Differentiation Effectors of Human Stem Cells

The combination of Radix Astragali and Radix Angelicae Sinensis attenuates the IFN-γ-induced immune destruction of hematopoiesis in bone marrow cells

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