Chimeric tymovirus-like particles displaying foot-and-mouth disease virus non-structural protein epitopes and its use for detection of FMDV-NSP antibodies

Masarapu, Hema; Singanallur, Nagendrakumar Balasubramanian; Reddivari, Yamini; Chandran, Dev; Lingala, Rajendra; Thiagarajan, D.; Parida, Satya; Paton, David; Srinivasan, V.A.

doi:10.1016/j.vaccine.2007.04.023

Cited by 29 publications

(15 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antibodies to FMDV NSP 3ABC were tested using Cedi test Ò FMDV-NS (Cedi-Diagnostics, The Netherlands) and an in-house indirect ELISA [9]. Serum antibody titres were measured [10] and expressed as the reciprocal of the final dilution of serum in the serum/virus mixture which neutralized an estimated 100 TCID 50 of virus at the 50% endpoint estimated according to the method of Kärber [11].…”

Section: Serologymentioning

confidence: 99%

Immune Response in Goats to Different Payloads of FMDV Monovalent Vaccine: Protection Against Virulent Challenge and Development of Carrier Status

et al. 2011

View full text Add to dashboard Cite

The relationship of Foot-and-Mouth Disease virus antigen payload and number of dose of vaccine conferring protection against virus challenge in goats was studied. Goats vaccinated with oil adjuvant Foot-and-Mouth Disease vaccines containing different antigen payloads with or without booster resisted virulent challenge at 21 days post-vaccination or 7 days after booster respectively. However, localized sub-clinical infection was observed in two vaccinated goats on 35 days post-challenge. RNA could be detected from 31.8% of vaccinated goats (10(2.69)-10(4.99) viral RNA copies per cotton swab of nasal secretions) on day 35 post-challenge. Since no live virus could be isolated after 5 days post-challenge, the risk of these animals transmitting the disease was probably very low. The finding showed that oil adjuvant Foot-and-Mouth Disease vaccines containing antigen payload of 1.88 μg may prevent or reduce the local virus replication at the oropharynx and shedding of virus from nasal secretions and thereby reduce the amount of virus released into the environment subsequent to exposure to live virus. This study also showed that goats with poor sero conversion to vaccination can be infected without overt clinical signs and became carriers like sheep.

show abstract

Section: Serologymentioning

confidence: 99%

Immune Response in Goats to Different Payloads of FMDV Monovalent Vaccine: Protection Against Virulent Challenge and Development of Carrier Status

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Antibodies to FMDV non-structural proteins (NSP) 3ABC were tested using Ceditest® FMDV-NS (Cedi-Diagnostics, The Netherlands) (Sorensen et al 1998) and an in-house indirect ELISA (Hema et al 2007). Briefly the in-house indirect ELISA was carried as follows: ELISA plates (Nunc® Maxisorp™, The Netherlands) were coated with purified chimeric proteins (50 ng/50 µl/well) in carbonate-bicarbonate coating buffer (pH 9.0).…”

Section: Non-structural Protein (Nsp) Antibodiesmentioning

confidence: 99%

“…The reaction was stopped with 1 M sulfuric acid (Merck, Germany) and read at 492 nm using ELISA plate reader (VERSAmax®, Molecular Devices, USA). The test samples were declared as positive or negative based on the cut-off value (Hema et al 2007). …”

Section: Non-structural Protein (Nsp) Antibodiesmentioning

confidence: 99%

Protection against direct in-contact challenge following foot-and-mouth disease vaccination in sheep and goats: the effect on virus excretion and carrier status

2010

Self Cite

View full text Add to dashboard Cite

The ability of foot-and-mouth disease (FMD) vaccine to protect sheep and goats from a homologous direct in-contact challenge and the effect on virus excretion from the nasal secretions and oropharynx was examined. An experimental oil adjuvant O(1) Manisa FMD vaccine protected sheep and goats from clinical disease from 7 days post vaccination following 24 hours of direct in-contact exposure to four infected donor sheep or goats. Goats required lower antibody titres for protection when compared with sheep. Protection from clinical disease did not prevent localized viral replication in goats and at least two goats had viral RNA detected on day 28 post challenge. Quantitative reverse transcriptase polymerase chain reaction showed that the level of virus replication shortly after direct in-contact challenge in oropharynx and nasal secretions of vaccinated animals was reduced by 100 and 1000 times respectively when compared with unvaccinated controls. The findings also show that after direct in-contact challenge, use of FMD vaccine will prevent or reduce local virus replication, thereby significantly reduce the amount of virus released into the environment in the all-important early post-exposure period. There is low risk of vaccinated animals transmitting disease as live virus could not be readily isolated.

show abstract

“…However, one of the major difficulties in implementing vaccination is the inability to distinguish vaccinated animals from infected/recovered animals, which may still be shedding virus. Currently, a number of assays specifically developed for this purpose are being validated (29,41), and the success of these assays is dependent on the use of purified vaccine antigen. A strategy using replication-deficient adenovirus 5 expressing FMDV antigens has been shown to provide early protection against homologous challenge (39).…”

mentioning

confidence: 99%

Induction of a Cross-Reactive CD8⁺T Cell Response following Foot-and-Mouth Disease Virus Vaccination

Guzman

Taylor

Charleston

et al. 2010

J Virol

View full text Add to dashboard Cite

Chimeric tymovirus-like particles displaying foot-and-mouth disease virus non-structural protein epitopes and its use for detection of FMDV-NSP antibodies

Cited by 29 publications

References 38 publications

Immune Response in Goats to Different Payloads of FMDV Monovalent Vaccine: Protection Against Virulent Challenge and Development of Carrier Status

Immune Response in Goats to Different Payloads of FMDV Monovalent Vaccine: Protection Against Virulent Challenge and Development of Carrier Status

Protection against direct in-contact challenge following foot-and-mouth disease vaccination in sheep and goats: the effect on virus excretion and carrier status

Induction of a Cross-Reactive CD8⁺T Cell Response following Foot-and-Mouth Disease Virus Vaccination

Contact Info

Product

Resources

About

Chimeric tymovirus-like particles displaying foot-and-mouth disease virus non-structural protein epitopes and its use for detection of FMDV-NSP antibodies

Cited by 29 publications

References 38 publications

Immune Response in Goats to Different Payloads of FMDV Monovalent Vaccine: Protection Against Virulent Challenge and Development of Carrier Status

Immune Response in Goats to Different Payloads of FMDV Monovalent Vaccine: Protection Against Virulent Challenge and Development of Carrier Status

Protection against direct in-contact challenge following foot-and-mouth disease vaccination in sheep and goats: the effect on virus excretion and carrier status

Induction of a Cross-Reactive CD8+T Cell Response following Foot-and-Mouth Disease Virus Vaccination

Contact Info

Product

Resources

About

Induction of a Cross-Reactive CD8⁺T Cell Response following Foot-and-Mouth Disease Virus Vaccination