Chimeric Structure of omp2 of Brucella from Pacific Common Minke Whales (Balaenoptera acutorostrata)

Ohishi, Kazue; Takishita, Kiyotaka; Kawato, Masaru; Zenitani, Ryoko; Bando, T.; Fujise, Yoshihiro; Goto, Yoshitaka; Yamamoto, Saburo; Maruyama, Tadashi

doi:10.1111/j.1348-0421.2005.tb03658.x

Cited by 7 publications

(9 citation statements)

References 17 publications

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“…Figure 3 suggests a position closer to the B. ceti group in agreement with the phenotypic behaviour [14], but the typing of more strains from Pacific waters [29-31] will be needed in order to achieve a more conclusive cluster analysis.…”

Section: Resultsmentioning

confidence: 84%

“…For the 295 strains analysed, using the MLVA-16 assay, 117 genotypes were resolved and seven clusters were identified, (i) two clusters almost exclusively composed of dolphin isolates, (ii) the predominantly porpoise cluster of strains (which also includes several strains isolated from dolphins), (iii) two main seal species clusters, (iv) the hooded seal cluster, and (v) the human isolate. The last cluster might correspond to Pacific Ocean isolates [29-31], which are underrepresented in the present collection. The hooded seal cluster of strains was composed of strains from Scotland and Norway.…”

Section: Discussionmentioning

confidence: 99%

“…In all these strains the porin omp2 genes were different from those from marine mammal strains isolated on European coasts [30]. Briefly, the omp2 genes of these isolates from the Pacific share common features with both marine mammal (from Europe) and terrestrial mammal strains [29]. Another interesting observation is that all the Pacific isolates investigated so far (including the three reported human cases) carry fragment I identified by IRS-PCR which is part of a putative genomic island specific for B. pinnipedialis [12].…”

Section: Discussionmentioning

confidence: 99%

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MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis

et al. 2009

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BackgroundSince 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. In order to investigate the genetic relationships within the marine mammal Brucella isolates and with reference to terrestrial mammal Brucella isolates, we applied in this study the Multiple Loci VNTR (Variable Number of Tandem Repeats) Analysis (MLVA) approach. A previously published assay comprising 16 loci (MLVA-16) that has been shown to be highly relevant and efficient for typing and clustering Brucella strains from animal and human origin was used.Results294 marine mammal Brucella strains collected in European waters from 173 animals and a human isolate from New Zealand presumably from marine origin were investigated by MLVA-16. Marine mammal Brucella isolates were shown to be different from the recognized terrestrial mammal Brucella species and biovars and corresponded to 3 major related groups, one specific of the B. ceti strains, one of the B. pinnipedialis strains and the last composed of the human isolate. In the B. ceti group, 3 subclusters were identified, distinguishing a cluster of dolphin, minke whale and porpoise isolates and two clusters mostly composed of dolphin isolates. These results were in accordance with published analyses using other phenotypic or molecular approaches, or different panels of VNTR loci. The B. pinnipedialis group could be similarly subdivided in 3 subclusters, one composed exclusively of isolates from hooded seals (Cystophora cristata) and the two others comprising other seal species isolates.ConclusionThe clustering analysis of a large collection of marine mammal Brucella isolates from European waters significantly strengthens the current view of the population structure of these two species, and their relative position with respect to the rest of the Brucella genus. MLVA-16 is confirmed as being a rapid, highly discriminatory and reproducible method to classify Brucella strains including the marine mammal isolates. The Brucella2009 MLVA-16 genotyping database available at http://mlva.u-psud.fr/ is providing a detailed coverage of all 9 currently recognized Brucella species.

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Section: Resultsmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis

et al. 2009

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Section: Contribution To Dao Special 3: 'Marine Vertebrate Zoonoses'mentioning

confidence: 99%

“…In contrast, Brucella isolates from dolphins and porpoises possessed no omp2a but 2 omp2b genes, thus identifying these isolates as B. cetaceae (Cloeckaert et al 2001). Brucella isolates from Pacific common minke whales Balaenoptera acutorostrata revealed omp2a and omp2b genes with motifs very similar to Atlantic marine strains as well as to terrestrial strains thus showing them to be chimeric between marine and terrestrial strains (Ohishi et al 2005).Oxidative metabolic profiles as well as variable number of tandem repeats (VNTR) typing and multilocus sequence analysis (MLSA) of marine Brucella isolates revealed the existence of 3 different groups, one comprising mainly pinniped isolates and the other 2 consisting of either porpoise or dolphin isolates (Groussaud et al 2007). The recently proposed classification of Brucella isolated from marine mammals is based on phenotypic and molecular methods and comprises B. pinnipedialis which prefer seal hosts, and B. ceti which prefer cetacean hosts (Foster et al 2007).…”

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confidence: 96%

Incidence of Brucella species in marine mammals of the German North Sea

Prenger‐Berninghoff¹,

Siebert²,

Stede³

et al. 2008

Dis. Aquat. Org.

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In this study, organ samples from 426 common seals Phoca vitulina, 298 harbour porpoises Phocoena phocoena, 34 grey seals Halichoerus grypus and 10 other marine mammals were assessed for the presence of Brucella species. Forty-seven common seals, 2 harbour porpoises and 1 grey seal were found to be positive for these bacteria. A total of 91 Brucella strains were successfully isolated, due to the fact that Brucella spp. were found in more than one organ sample in 15 animals. The primary organ in which the bacteria were present was the lung. In addition, 2 strains were isolated from lungworms (Parafilaroides spp.). Forty-nine of the isolated strains were selected for further analysis using conventional phenotyping methods. Molecular characterisation was carried out by analysing the IS711 and omp2 loci. With respect to the distribution of the IS711 loci in the genome, the 49 field isolates differed strongly from the terrestrial Brucella species and marginally from the marine Brucella reference strain NCTC12890. Based on the results of the PCR restriction fragment length polymorphism (PCR-RFLP) investigation of the omp2 locus, the majority of the Brucella field isolates were classified as B. pinnipediae, recently proposed B. pinnipedialis, possessing 1 omp2a gene and 1 omp2b gene. Two field isolates revealed the presence of 2 omp2a genes, as has been described for Brucella ovis. To our knowledge, these results confirm for the first time the presence of Brucella species in the marine mammal population of the German North Sea. These findings highlight the need for additional research on the relevance of these Brucella species for marine hosts and their environment. KEY WORDS: Brucella species · Marine mammals · Phenotypic and molecular investigation · German North Sea Resale or republication not permitted without written consent of the publisher OPEN PEN ACCESS CCESS Contribution to DAO Special 3: 'Marine vertebrate zoonoses'Dis Aquat Org 81: [65][66][67][68][69][70][71] 2008 marine Brucella isolates (Clavareau et al. 1998, Bricker et al. 2000, Cloeckaert et al. 2000.A further distinction of the marine Brucella species is possible when analysing the genus-specific omp2-locus. This locus consists of the 2 closely related genes omp2a and omp2b. Both share an approximated 85% DNA homology; they are separated by 900 bp and oriented in opposite directions (Ficht et al. 1989). Current data suggest the gene arrangement is conserved in all Brucella species. Therefore, distinction between the Brucella species and some of their biovars can be performed by PCR-restiction fragment length polymorphism (PCR-RFLP) analysis (Cloeckaert et al. 1995). In a further study, Cloeckaert et al. (2001) obtained data which led to a subdivision of the marine Brucella isolates into 2 groups. The strains isolated from seals and 1 otter possessed 1 omp2a and 1 omp2b gene, representing the formerly proposed species B. pinnipediae. In contrast, Brucella isolates from dolphins and porpoises possessed no omp2a but 2 omp2b genes, thus iden...

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Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens

Whatmore¹

2009

Infection, Genetics and Evolution

234

227

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Chimeric Structure of omp2 of Brucella from Pacific Common Minke Whales (Balaenoptera acutorostrata)

Cited by 7 publications

References 17 publications

MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis

MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis

Incidence of Brucella species in marine mammals of the German North Sea

Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens

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