Chimeric Quinol Oxidases Expressed in Paracoccus Denitrificans

Winterstein, Christine; Richter, O.-M. H.; Ludwig, Bernd

doi:10.1007/978-94-011-5133-7_19

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…Given the close relationship of the P. denitrificans quinol oxidase to its E. coli homologue, a similar spatial structure can be inferred from these results. Another indication of their structural similarity is the retention of some enzymatic activity (about 20% both in membranes and in the isolated complex compared to the native P. denitrificans quinol oxidase) after replacement of the qoxA gene coding for subunit II of the P. denitrificans enzyme by the equivalent gene, cyoA, from the bo 3 -type quinol oxidase of E. coli (324). It is not yet clear whether the chimeric quinol oxidase retains the ability to translocate one proton across the membrane per electron transferred to oxygen, as do the parent oxidases.…”

Section: Genes Of Oxygen Respirationmentioning

confidence: 99%

Molecular Genetics of the Genus Paracoccus : Metabolically Versatile Bacteria with Bioenergetic Flexibility

Baker¹,

Ferguson

Ludwig

et al. 1998

Microbiol Mol Biol Rev

204

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SUMMARY Paracoccus denitrificans and its near relative Paracoccus versutus (formerly known as Thiobacilllus versutus) have been attracting increasing attention because the aerobic respiratory system of P. denitrificans has long been regarded as a model for that of the mitochondrion, with which there are many components (e.g., cytochrome aa3 oxidase) in common. Members of the genus exhibit a great range of metabolic flexibility, particularly with respect to processes involving respiration. Prominent examples of flexibility are the use in denitrification of nitrate, nitrite, nitrous oxide, and nitric oxide as alternative electron acceptors to oxygen and the ability to use C1 compounds (e.g., methanol and methylamine) as electron donors to the respiratory chains. The proteins required for these respiratory processes are not constitutive, and the underlying complex regulatory systems that regulate their expression are beginning to be unraveled. There has been uncertainty about whether transcription in a member of the alpha-3 Proteobacteria such as P. denitrificans involves a conventional ς70-type RNA polymerase, especially since canonical −35 and −10 DNA binding sites have not been readily identified. In this review, we argue that many genes, in particular those encoding constitutive proteins, may be under the control of a ς70 RNA polymerase very closely related to that of Rhodobacter capsulatus. While the main focus is on the structure and regulation of genes coding for products involved in respiratory processes in Paracoccus, the current state of knowledge of the components of such respiratory pathways, and their biogenesis, is also reviewed.

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Section: Genes Of Oxygen Respirationmentioning

confidence: 99%

Molecular Genetics of the Genus Paracoccus : Metabolically Versatile Bacteria with Bioenergetic Flexibility

Baker¹,

Ferguson

Ludwig

et al. 1998

Microbiol Mol Biol Rev

204

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Expression of the Escherichia coli cyo operon in Paracoccus denitrificans results in a fully active quinol oxidase of unexpected heme composition

et al. 1998

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The cyo operon coding for the membrane-bound bo Qtype quinol oxidase of Escherichia coli has been expressed in a Paracoccus denitrificans strain deleted in its endogenous ba Q quinol oxidase. Using the P. denitrificans qox promoter, the His tagged protein complex is synthesized to a level comparable to that in E. coli and the enzyme purified in a single step on a metalchelating column. Whereas the activity of the isolated complex matches that of the oxidase purified directly from E. coli, the heterologously expressed oxidase does not show the characteristic heme composition but now carries heme a in its binuclear site.z 1998 Federation of European Biochemical Societies.

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Chimeric Quinol Oxidases Expressed in Paracoccus Denitrificans

Cited by 2 publications

References 27 publications

Molecular Genetics of the Genus Paracoccus : Metabolically Versatile Bacteria with Bioenergetic Flexibility

Molecular Genetics of the Genus Paracoccus : Metabolically Versatile Bacteria with Bioenergetic Flexibility

Expression of the Escherichia coli cyo operon in Paracoccus denitrificans results in a fully active quinol oxidase of unexpected heme composition

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