Chilling tolerance in Nicotiana tabacum induced by seed priming with putrescine

Xu, Shengchun; Hu, Jin; Li, Yongping; Ma, Wenguang; Zheng, Yang; Zhu, Shuijin

doi:10.1007/s10725-010-9528-z

Cited by 80 publications

(44 citation statements)

References 34 publications

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“…Correlation coefficients between PAs and ethylene were not significant in NaCl-stressed plants and suggest that the predominant effect was synthesis of ethylene (as a signal molecule), in those conditions. Changes in activities of the antioxidant enzymes SOD, POD, CAT and GR observed in the tolerant and sensitive genotypes were related to the mobilization of the protective system in stress conditions caused by ROS (Xu et al 2011). Correlation coefficients calculated for SOD, the enzyme engaged as the first in the defense mechanism against oxygen radicals (Alscher et al 2002) and other enzymes revealed significant increases in the leaves of PEG-treated plants (r = 0.6965, 0.8365 and -0.8950 for CAT, POD and GR, respectively, p \ 0.05).…”

Section: Discussionmentioning

confidence: 97%

Relationships between polyamines, ethylene, osmoprotectants and antioxidant enzymes activities in wheat seedlings after short-term PEG- and NaCl-induced stresses

et al. 2012

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Contents of ethylene, osmoprotectants, levels and forms of polyamines (PAs) and activities of antioxidant enzymes in the leaves and roots were investigated for five wheat cultivar seedlings (differing in drought tolerance) exposed to osmotic stress (-1.5 MPa). Stress was induced by 2-day-long treatment of plants with polyethylene glycol 6000 (PEG) or NaCl added to hydroponic cultures. Nawra, Parabola and Manu cv. (drought tolerant) showed a marked increase in osmoprotectors (proline and soluble carbohydrates, mainly glucose, saccharose and maltose), free PAs (putrescine Put, spermidine Spd and spermine Spm) and Spd-conjugated levels, in both leaves and roots, after PEGtreatments. Radunia and Raweta (drought sensitive) exhibited smaller changes in the content of these substances. The analysis of enzymes involved in proline metabolism revealed the glutamate as a precursor of proline synthesis in PEGinduced stress conditions. The increase in the activity of antioxidative enzymes, especially catalase and peroxidases, was characteristic for tolerant wheat plants, but for sensitive ones, a decrease in superoxide dismutase and an increase in mainly glutathione reductase activities were observed. After NaCl-treatment smaller changes of all biochemical parameters were registered in comparison with PEG-induced stress. Exceptions were the higher values of ethylene content and a significant increase in saccharose, raffinose and maltose levels (only in stress sensitive plants). The proline synthesis pathway was stimulated from both glutamate and ornithine precursors. These results suggest that the accumulation of inorganic ions in NaCl-stressed plants may be involved in protective mechanisms as an additional osmoregultor. Thus, a weaker stressogenic effect as determined as water deficit by leaf relative water content and relative dry weight increase rate and differences in metabolite synthesis in comparison with PEG stress was observed. Proline seems to be the most important osmo-protector in osmotic stress initiated by both PEG and NaCl. The synthesis of sugars and PAs may be stimulated in a stronger stress conditions (PEG).

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Section: Discussionmentioning

confidence: 97%

Relationships between polyamines, ethylene, osmoprotectants and antioxidant enzymes activities in wheat seedlings after short-term PEG- and NaCl-induced stresses

et al. 2012

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“…Polyamines play regulatory roles in various important cellular processes including cell division, differentiation and proliferation, cell death, DNA and protein synthesis and gene expression alongside the stabilizing macromolecular structures (Igarashi and Kashiwagi, 2000;Childs et al, 2003;Kusano et al, 2008). Xu et al (2011) also indicated that germination, seedling length and dry weight of chilling-sensitive tobacco varieties enhanced by putrescine treatments under chilling conditions.…”

Section: Discussionmentioning

confidence: 96%

“…Iqbal and Ashraf (2005) demonstrated that wheat (Triticum aestivum L.) seed priming with spermidine, spermine and putrescine increased photosynthetic capacity and also wheat growth under saline conditions. Xu et al (2011) revealed that tobacco (Nicotiana tabacum L.) seed priming with putrescine improved germination percentages, germination index, seedling length and dry weight in comparison with controls which caused chilling tolerance. Farooq et al (2007) showed that sunflower (Helianthus annuus L.) seeds priming with polyamines (spermidine and putrescine) increased germination percentages, root and shoot length, early seedling growth and decreased emergence time.…”

Section: Introductionmentioning

confidence: 99%

“…Several study have shown that exogenous application of spermidine as an important polyamine can play an important role in recovering the plasma membrane damage and electrolyte leakage (EL) in rice genotypes and cucumber in response to environmental stresses like chilling, salinity and drought (Kubis, 2008;Roy et al, 2005). Xu et al (2011) showed that the amount of seedling leaf relative water content (RWC) improved under chilling stress via seed priming with putrescine probably by osmotic adjustment or might alter in cell wall stretchiness. Cao et al (2008) reported that seed soaking with putrescine improves chilling tolerance of maize seeds.…”

Section: Introductionmentioning

confidence: 99%

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Alleviating harmful effects of chilling stress on rice seedling via application of spermidine as seed priming factor

Pouramir-Dashtmian¹,

Khajeh-Hosseini²,

Esfahani³

2014

Afr. J. Agric. Res.

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Chilling stress is a major limiting factor for rice production in many parts of the world. The study was carried out in the Seed Research Laboratory of the Department of Crop Science, Ferdowsi University of Mashhad in summer 2011. Rice (cv. Khazar) seeds were soaked in 50, 150 and 300 mg L -1 spermidine aerated solutions for 48 h and then dried back to the original moisture content and were sown in three temperatures (28°C as normal, 12 and 8°C as chilling stress). Chilling stress (8°C) reduced the root (39%) and shoot (52%) growth in untreated seeds, while the reduction of 11% root growth and 20% shoot growth was observed when the seeds were primed with 300 mg L -1 spermidine solution. The electrolyte leakage (EL) of the seedling leaves significantly increased in low temperatures as it was 67% in 8°C in compared with normal condition. Chilling stress significantly increased superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activity in rice seedlings leaves though this increase was not significant for glutathione reductase (GR). Seed priming with spermidine had a positive effect on seedlings leaves antioxidant activity in every temperature conditions. As in 8°C, the sharp increase (73%) in SOD activity was occurred at 300 mg L -1 spermidine solution and it was 23 and 46% for CAT and APX, respectively. In general, seed priming with spermidine alleviated the chilling effect, probably as a result of activating antioxidants production processes and membrane stabilizing in cellular structures.

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“…Chilling injury in tobacco (Nicotiana tabacum L.) is a common problem in China, especially in the southwest region, and 90% of tobacco seeds are currently pelleted in China (Xu et al 2011). Therefore, tobacco seed pellets with chilling-tolerant traits are very useful.…”

Section: Introductionmentioning

confidence: 99%

“Intelligent” seed pellets may improve chilling tolerance in tobacco

Cui

Guan

et al. 2012

Frontiers in Life Science

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Tobacco seeds and seedlings often encounter low temperature stress in China. An 'intelligent' tobacco seed pellet was developed to improve chilling tolerance by adding thermo-sensitive PNIPAAm-co-BMA (poly(N -isopropylacrylamide-cobutyl methacrylate)) hydrogel. PNIPAAm-co-BMA can detect changes in environmental temperature and change its reticular structure accordingly. In addition, the release rate of salicylic acid (SA) loaded in PNIPAAm-co-BMA hydrogel is changed. Tobacco seeds of two different chilling-tolerant tobacco varieties were pelleted by SA-loaded PNIPAAm-co-BMA, and then the germination characteristics, seedling quality and some physiological parameters of the seeds and seedlings were measured after a chilling stress at 11 • C for 5 d. The results showed that the germination percentage (GP), germination index (GI), root length, seedling dry weight and the activities of POD (peroxidase) were significantly increased and the concentration of MDA (malondialdehyde) was significantly decreased in the 'intelligent' seed pellets compared with the seeds without SA and PNIPAAm-co-BMA in both varieties. The results showed that the 'intelligent' seed pellets had thermo-sensitive traits; they enhanced and prolonged the efficiency and acting time of loaded SA. This indicated that the 'intelligent' seed pellets pelleted with SA-loaded PNIPAAm-co-BMA could be used to improve chilling tolerance.

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Chilling tolerance in Nicotiana tabacum induced by seed priming with putrescine

Cited by 80 publications

References 34 publications

Relationships between polyamines, ethylene, osmoprotectants and antioxidant enzymes activities in wheat seedlings after short-term PEG- and NaCl-induced stresses

Relationships between polyamines, ethylene, osmoprotectants and antioxidant enzymes activities in wheat seedlings after short-term PEG- and NaCl-induced stresses

Alleviating harmful effects of chilling stress on rice seedling via application of spermidine as seed priming factor

“Intelligent” seed pellets may improve chilling tolerance in tobacco

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