Chilling ovarian fragments during transportation improves viability and growth of goat preantral follicles cultured in vitro

Chaves, Roberta Nogueira; Martins, F.S.; Saraiva, M.V.A.; Celestino, J.J.H.; Lopes, C.A.P.; Correia, João Carlos; Verde, Isabel Bezerra Lima; Matos, Maria Helena Tavares de; Báo, Sônia Nair; Name, Khesller Patrícia Olázia; Campello, C.C.; Silva, J. R. V.; Figueiredo, J.R.

doi:10.1071/rd07195

Cited by 126 publications

(77 citation statements)

References 21 publications

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“…Wang et al (2011) demonstrated that 15 ºC was better than 30 ºC and 35 ºC in terms of reduction of the apoptosis index, but there were no significant differences in cleavage and blastocyst development. The high maturation rate that was achieved at 4 ºC may be related to the low temperature, which should reduce cellular metabolism (Chaves et al, 2008), minimize energy consumption, and slow down the tissue autolysis rate (Salehi et al, 2004). In addition, the efficiency of enzymes and incidence of apoptosis decreases at low temperatures (Pedersen et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, improper transporting and preserving conditions might cause failure in oocyte maturation and developmental competence. A change in the storage temperature and preservation solution during ovary transport has great effect on the developmental competence of oocytes following in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) Kim et al, 2006;Chaves et al, 2008;Lopes et al, 2009). Previous researchers showed that oocyte quality affects the IVP efficiency in most species.…”

Section: Introductionmentioning

confidence: 99%

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HEPES buffer in ovary-transportation medium influences developmental competence of cattle oocytes

Bohlooli¹,

Bozoglu²,

Cedden³

2016

SA J. An. Sci.

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This study was conducted to investigate the effects of ovary transportation in a semi-complex medium containing HEPES at different temperatures on the developmental competence and the quality of in vitroproduced embryos. The cattle ovaries were transported in normal saline (NS), phosphate buffer saline (PBS), K simplex optimization medium (KSOM), Chatot-Ziomek-Bavister medium (CZB) and Charles Rosenkrans medium (CR1) at various temperatures (38 ºC, 25 ºC and 4 ºC). The developmental competence of retrieved cumulus oocyte complexes (COCs) was evaluated by maturation, fertilization, morula and blastocyst formation and numbers of inner cell mass (ICM) and trophectoderm (TE) cells. The COC maturation rate was affected by medium and temperature. It was found that 4 ºC resulted in a higher maturation (81.0 ± 4.75) rate than other transportation temperatures. The CR1 (80.5 ± 6.66) and KSOM (80.2 ± 6.15) gave a better maturation rate than the others. Fertilization rate, which was evaluated by cleavage rate, was not affected by transportation temperature. However, the transporting medium had a significant effect on the fertilization rate. Moreover, CR1 (43.6 ± 4.60), KSOM (43.2 ± 4.86) and CZB (41.1 ± 4.86) media gave higher percentages of cleaved embryos. There was no significant difference in morula and blastocyst formation rate or in ICM and TE cell counts regarding transportation factors. In conclusion, the transport of ovaries in CR1 at 4 ºC is effective for maintaining early developmental competence of cattle oocytes.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

HEPES buffer in ovary-transportation medium influences developmental competence of cattle oocytes

Bohlooli¹,

Bozoglu²,

Cedden³

2016

SA J. An. Sci.

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“…Chaves et al (2008) have shown that ovarian tissue transportation in Minimal Essential Medium (MEM) at 4°C for up to 4 h maintained the percentages of morphologically normal follicles similar to those observed in fresh tissues even after 7 days of in vitro culture. However, the use of synthetic media (e.g.…”

Section: Introductionmentioning

confidence: 91%

“…Then, one ovarian fragment was taken randomly and fixed for histological and TUNEL analyses (fresh control). The other 12 fragments were randomly placed into tubes containing 5 mL MEM supplemented with antibiotics (100 µg/mL penicillin and 100 µg/mL streptomycin; control medium; CHAVES et al, 2008) or different concentrations of M. nigra extract (0.025; 0.5 or 0.1 mg/mL), obtained from the dilution in 0.9% saline solution, and stored at 4ºC for 6, 12 or 24 h (simulating transport) ( Figure 1). The temperature was maintained using thermos boxes with ice.…”

Section: Ovaries Collection and In Vitro Preservationmentioning

confidence: 99%

Effect of ovarian tissue storage in Morus nigra extract on the morphology and DNA fragmentation of ovine preantral follicles

Cavalcante

Barberino

Gouveia

et al. 2017

SCA

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This study demonstrated the effect of Morus nigra leaf extract during ovine ovarian tissue transportation on the survival and apoptosis of preantral follicles in vitro. High Performance Liquid Chromatography (HPLC) was used to determine the fingerprint chromatogram of the crude ethanolic extract. Four pairs of ovaries from four sheep were collected. The ovarian cortex was fragmented and one fragment was fixed in 10% buffered formaldehyde and processed for histological and TUNEL analyses (fresh control). The other fragments were placed in Minimal Essential Medium (MEM -control medium) or M. nigra extract (0.025; 0.05 or 0.1 mg/mL) and stored (simulating transport) at 4ºC for 6, 12 or 24 h. Preserved fragments (6 h) were also destined to histological and TUNEL analyses. HPLC analysis confirmed the presence of antioxidant compounds (rutin, isoquercetin e kaempferitrin) in the extract. There was a decrease (P < 0.05) in the percentage of morphologically normal preantral follicles after preservation in all treatments compared to the fresh control. The percentage of normal preantral follicles after preservation in M. nigra at 0.05 mg/mL for 6 h was higher (P < 0.05) than in MEM or 0.025 mg/ mL M. nigra and similar (P > 0.05) to 0.1 mg/mL of the extract. Apoptosis increased (P < 0.05) after conservation for 6 h in all treatments compared to the fresh control. Moreover, TUNEL positive cells decreased (P < 0.05) after preservation in 0.05 or 0.1 mg/mL M. nigra compared to MEM or 0.025 mg/ mL M. nigra. In conclusion, 0.05 mg/mL M. nigra extract can be used as a preservation medium for ovine ovarian tissue at 4°C for up to 6 h. ResumoEste estudo demonstrou o efeito do extrato das folhas de Morus nigra durante o transporte na sobrevivência e apoptose de folículos pré-antrais in vitro. A CLAE (Cromatografia Líquida de Alta Eficiência) foi usada para determinar a impressão digital cromatográfica do extrato etanólico. Foram coletados 4 pares de ovários de 4 ovelhas. O córtex ovariano foi fragmentado e um dos fragmentos foi fixado em formaldeído tamponado a 10% e destinado às análises histológica e de TUNEL (controle fresco). Os demais fragmentos foram colocados em Meio Essencial Mínimo (MEM -meio controle) ou extrato de M. nigra (0,025, 0,05 ou 0,1 mg/mL) e conservados (simulando transporte) à 4 ºC por 6, 12 ou 24 h. Os fragmentos conservados (6 h) também foram destinados às análises histológica e de TUNEL. A análise de CLAE confirmou a presença de compostos antioxidantes (rutina, isoquercetina e canferitrina) no extrato. Houve uma redução (P < 0,05) na percentagem de folículos pré-antrais morfologicamente normais após conservação em todos os tratamentos em comparação com o controle fresco. A percentagem de folículos pré-antrais normais após conservação em M. nigra a 0,05 mg/mL por 6 h foi maior (P < 0,05) do que em MEM ou M. nigra a 0,025 mg/mL e similar (P > 0,05) ao extrato a 0,1 mg/mL. A apoptose aumentou (P < 0,05) após conservação durante 6 h em todos os tratamentos em comparação com o controle fresco. Além diss...

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“…Immediately postmortem, the ovaries were washed in 70% alcohol for 10 seconds followed by two washes in Minimum Essential Medium (MEM) plus HEPES (MEM HEPES) supplemented with 100 μg/ml penicillin and 100 μg/ml streptomycin. The ovary pairs were transported within 1 hour to the laboratory in MEM at 4°C (Chaves et al 2008).…”

Section: Source Of Ovariesmentioning

confidence: 99%

Bone Morphogenetic Protein-6 (BMP-6) induces atresia in goat primordial follicles cultured in vitro

et al. 2010

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770Pesq. Vet. Bras. 30(9):770-776, setembro 2010 RESUMO.-[A Proteína Morfogenética Óssea-6 (BMP-6) induz a atresia em folículos primordiais caprinos cultivados in vitro.] O presente estudo investigou os efeitos da proteína morfogenética óssea-6 (BMP-6) no desenvolvimento in vitro de folículos primordiais caprinos. Amostras de córtex ovariano de cabras foram cultivados por 1 ou 7 dias em Meio Essencial Mínimo (meio controle) suplementado com diferentes concentrações de BMP-6. As taxas de sobrevivência, ativação e crescimento foram avaliadas por histologia clássica e microscopia eletrônica de transmissão (MET). Após 7 dias de cultivo, a análise histológica demonstrou que a BMP-6 aumentou o percentual de folículos primordiais degenerados no dia 7 quando comparados ao controle fresco (D0). Além disso, houve um aumento significativo do diâmetro folicular e oocitário em ambos os períodos de cultivo em todos os tratamentos na presença de BMP-6. Com a progressão do cultivo do dia 1 para o dia 7, nos tratamentos com 1 ou 50ng/ml de BMP-6, foi observado um aumento significativo no diâmetro folicular. Entretanto, contrário ao observado no meio controle, a MET revelou que os folículos cultivados nesses tratamentos apresentavam sinais evidentes de atresia. Em conclusão, esse estudo demonstrou que a BMP-6 afeta negativamente a sobrevivência e a ultra-estrutura de folículos primordiais caprinos. This study investigated the effects of bone morphogenetic protein 6 (BMP-6) on in vitro primordial follicle development in goats. Samples of goat ovarian cortex were cultured in vitro for 1 or 7 days in Minimum Essential Medium (control medium) supplemented with different concentrations of BMP-6. Follicle survival, activation and growth were evaluated through histology and transmission electron microscopy (TEM). After 7 days of culture, histological analysis demonstrated that BMP-6 enhanced the percentages of atretic primordial follicles when compared to fresh control (day 0). Nevertheless, BMP-6 increased follicular and oocyte diameter during both culture periods. As the culture period progressed from day 1 to day 7, a significant increase in follicle diameter was observed with 1 or 50ng/ml BMP-6. However, on the contrary to that observed with the control medium TEM revealed that follicles cultured for up to 7 days with 1 or 50ng/ml BMP-6 had evident signs of atresia. In conclusion, this study demonstrated that BMP-6 negatively affects the survival and ultrastructure of goat primordial follicles.

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Chilling ovarian fragments during transportation improves viability and growth of goat preantral follicles cultured in vitro

Cited by 126 publications

References 21 publications

HEPES buffer in ovary-transportation medium influences developmental competence of cattle oocytes

HEPES buffer in ovary-transportation medium influences developmental competence of cattle oocytes

Effect of ovarian tissue storage in Morus nigra extract on the morphology and DNA fragmentation of ovine preantral follicles

Bone Morphogenetic Protein-6 (BMP-6) induces atresia in goat primordial follicles cultured in vitro

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