Chilling-enhanced photooxidation: The production, action and study of reactive oxygen species produced during chilling in the light

Wise, Robert R.

doi:10.1007/bf00032579

Cited by 304 publications

(214 citation statements)

References 166 publications

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“…This observation is consistent with our results showing a link between tolerance to chilling-induced photooxidation and ELIPs. Such intraspecific analysis of ELIP synthesis should be extended to other plants of agronomic interest, especially crop plants of tropical origin (e.g., maize, cucumber, or tomato), which are very sensitive to photodamage at chilling temperature (35). ELIPs constitute a plant defense system in light stress conditions, which have the potential to become new selection markers for the identification and the development of crop plants more tolerant to photooxidative stress conditions.…”

Section: Resultsmentioning

confidence: 99%

“…These primers introduced a NotI and a BamHI restriction site, respectively, upstream of the ATG start codon and downstream of the stop codon. These restriction sites were used to clone the cDNA between the promoter (cauliflower mosaic virus 35 S͞⍀ leader) and the polyadenylation signal of pRT⍀͞Not intermediate vector. These expression cassettes were then subcloned in the binary vector pGPTV HPT (21).…”

Section: Methodsmentioning

confidence: 99%

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Early light-induced proteins protect Arabidopsis from photooxidative stress

Hutin

Nussaume

Moise

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

297

250

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The early light-induced proteins (ELIPs) belong to the multigenic family of light-harvesting complexes, which bind chlorophyll and absorb solar energy in green plants. ELIPs accumulate transiently in plants exposed to high light intensities. By using an Arabidopsis thaliana mutant (chaos) affected in the posttranslational targeting of light-harvesting complex-type proteins to the thylakoids, we succeeded in suppressing the rapid accumulation of ELIPs during high-light stress, resulting in leaf bleaching and extensive photooxidative damage. Constitutive expression of ELIP genes in chaos before light stress resulted in ELIP accumulation and restored the phototolerance of the plants to the wild-type level. Free chlorophyll, a generator of singlet oxygen in the light, was detected by chlorophyll fluorescence lifetime measurements in chaos leaves before the symptoms of oxidative stress appeared. Our findings indicate that ELIPs fulfill a photoprotective function that could involve either the binding of chlorophylls released during turnover of pigment-binding proteins or the stabilization of the proper assembly of those proteins during high-light stress.L ight is essential for plants through photosynthetic carbon assimilation. However, when absorbed light exceeds the photosynthetic capacities, reactive O 2 species are generated in the chloroplasts, causing oxidative damage to proteins, lipids, and photosynthetic pigments (1, 2). This effect is amplified by environmental stresses such as low temperature or drought, for example, that inhibit the photosynthetic activity, leading to strong yield reduction in crops. In green plants, solar energy is collected by chlorophyll-and carotenoid-binding lightharvesting complexes (LHCs), which are encoded by a multigene family of LHC genes. The expression of these genes is tightly regulated by light (2-4). High light intensities inhibit transcription of LHC genes and activate synthesis of the early lightinduced proteins (ELIPs), a class of proteins structurally related to the LHCs (5). The ELIPs are predicted to have three transmembrane helices, and they have sequence similarity to the LHCs in the central pair of helices (6, 7). The similarity is not only at the sequence level, however, because both LHCs and ELIPs bind chlorophyll and carotenoids (8). The ELIPs differ from the LHCs by their transient expression under high-light stress (5). Recently, a number of ELIP-type polypeptides, containing LHC motifs and inducible by high light, have been discovered in vascular plants: the one-helix high-light-induced proteins (9) and the two-helix stress-enhanced proteins (10).The physiological role of the ELIPs in vascular plants has not yet been elucidated, although there have been several suggestions (11)(12)(13)(14). The induction of ELIPs by high light intensities suggests a role in the acclimation to light stress rather than a light-harvesting function, but this has not yet been demonstrated. ELIP antisense transgenic tobacco plants did not exhibit any phenotype of sensitivity to high ...

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Early light-induced proteins protect Arabidopsis from photooxidative stress

Hutin

Nussaume

Moise

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

297

250

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“…There is growing evidence that in plants subjected to environmental stress, the balance between the production of activated oxygen species and the quenching activity of antioxidants is upset, which often results in oxidative damage Hendry et al, 1992 ;De Voss et al, 1992 ;Smirnoff, 1993 ;Wise, 1995 ;Karpinski et al, 1997). It has also been reported that plants with high levels of antioxidants, whether constitutive or induced, have a greater resistance to such oxidative damage Pastori & Trippi, 1992 ;Scandalios, 1993 ;Foyer et al, 1994 ;Rennenberg & Polle, 1994 ;McKersie et al, 1996 ;.…”

mentioning

confidence: 99%

Response of antioxidant systems and leaf water relations to NaCl stress in pea plants

et al. 1999

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A pea (Pisum sativum cv. Puget) cultivar was grown on a medium containing different NaCl concentrations (0-160 mol m −$ ) in order to study the effects of salt stress on leaf water relations and on the activity of antioxidant enzymes. NaCl stress caused a rapid decline in chlorophyll content. Both leaf water (ψ l ) and osmotic potentials (ψ s ) decreased progressively with the severity of the stress (from 90-160 mol m −$ NaCl) whereas leaf turgor pressure (ψ p ) increased in treated plants. Pea leaves contained an iron-containing superoxide dismutase (Fe-SOD) isozyme in chloroplasts alongside a copper-zinc-containing (CuZn-SOD) form (CuZn-SOD II). The lowest NaCl concentration (70 mol m −$ ) had no effect on the activity of these antioxidant enzymes while higher concentrations (110-130 mol m −$ ) enhanced the activity of cytosolic CuZn-SOD I and chloroplastic CuZn-SOD II as well as that of mitochondrial and\or peroxisomal manganese-containing superoxide dismutase (Mn-SOD). These inductions were matched by increases in the activity of ascorbate peroxidase (APX) and monodehydroascorbate reductase (MDHAR). The increased activities coincided with decreased stomatal conductance and were unaffected by the severity of stress except in the case of CuZn-SOD II which fell to control values under the highest stress conditions (140-160 mol m −$ NaCl), when a concomitant increase in chloroplastic Fe-SOD activity was observed. Glutathione reductase (GR) and dehydroascorbate reductase (DHAR) activities were only induced under severe NaCl stress (130-160 mol m −$ ) and were accompanied by losses in the ascorbate and glutathione pools, lower ASC\DHA and GSH\GSSG ratios and increases in GSSG. Electron microscopy showed that the thylakoidal structure of the chloroplasts became disorganized and their starch content decreased in plants treated with 160 mol m −$ NaCl. Overall, the results suggest that salt stress is accompanied by oxidative stress, perhaps at the cell compartment level. The capacity of Puget cultivar to ensure cell turgor and to enhance the activity of enzymes involved in the defence against oxidative stress seems to be important in determining adaptation to moderate NaCl stress conditions. In plants exposed to severe NaCl stress (130-160 mol m −$ ) it seems that such resistance to oxidative stress is overcome, which might contribute to the deleterious effects of salt and significant growth reduction in these conditions.

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“…Photo-oxidative stress in plants can result in high levels of singlet oxygen ( 1 Σg + ) and superoxide (O 2 •-) being produced, either from direct UV radiation on oxygen or the leakage of light energy onto oxygen from chlorophyll when the carotenoid pigments become saturated (Wise, 1995). Plants are highly susceptible to photooxidative stresses at low temperatures when exposed to strong light conditions.…”

Section: Wwwintechopencommentioning

confidence: 99%

“…When the ability of antioxidants to quench the formation of ROS and the recycling of antioxidants is reduced, greater damage occurs to the chloroplast through lipid peroxidation, inactivation of photosynthetic proteins and loss of pigments (i.e. bleaching) (Wise, 1995;Wise & Naylor, 1987). Damage to the chloroplast during chilling stress has been shown to severely impede growth rates (Partelli et al, 2009); therefore, reducing the damage that occurs to plant cells due to low temperature oxidative stress is vitally important for improving survival and recovery in cryopreservation.…”

Section: Wwwintechopencommentioning

confidence: 99%