Chilli veinal mottle virus HCPro interacts with catalase to facilitate virus infection in Nicotiana tabacum

Yang, Ting; Qiu, Ling; Huang, Wanying; Xu, Qianyi; Zou, Jialing; Peng, Qiao; Lin, Honghui; Xi, Dehui

doi:10.1093/jxb/eraa304

Cited by 41 publications

(45 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, we demonstrated that O 2 − , H 2 O 2 and dead cells accumulated along the veins of VIS leaves, indicating that SMoV and SVBV may trigger ROS accumulation and cause cell death in VIS plants. These results are consistent with those of Conti et al (2012) and Yang et al (2020), who showed that tobacco mosaic virus and chilli veinal mottle virus stimulated ROS accumulation, and infected leaves underwent cell death. Correspondingly, we observed a higher CAT activity in VIS leaves than in control leaves, suggesting that the defense response of VIS plants involves the induction of CAT to eliminate H 2 O 2 .…”

Section: Discussionsupporting

confidence: 92%

Effect of strawberry vein banding virus and strawberry mottle virus co-infection on the growth and development of strawberry

Fan¹,

Song²,

Khoo³

et al. 2022

Biocell

View full text Add to dashboard Cite

Strawberry mottle virus (SMoV) and strawberry vein banding virus (SVBV) cause diseases on strawberry plants, but the effect of coinfection of SMoV and SVBV on the growth, development, and defense system of strawberry (Fragaria × ananassa Duchesne) remains unknown. We investigated the effect of SMoV and SVBV coinfection on strawberry cultivar 'Benihope'. The results showed that stem diameter, leaf size, leaf number, relative chlorophyll content, total chlorophyll content, photosynthetic parameters, and stomatal aperture of SMoV and SVBV co-infected strawberry (VIS) plants were in a weaker level than uninfected control plants, indicating that viruses inhibited the growth and photosynthesis of strawberry plants. Furthermore, the initiation of flowering and fruiting stages of VIS plants were delayed by about three weeks compared with the controls, and the fruiting period was shortened, demonstrating that the reproduction of VIS plants was inhibited. Fruit quality was damaged in VIS plants due to a significant increase in fruit firmness and titratable acidity and decrease in total soluble solid content than control fruits. More dead cells and H 2 O 2 accumulated along the veins of VIS leaves, and the content of abscisic acid and catalase activity significantly increased, whereas anthocyanin content was lower than that of control plants. The results demonstrate that SVBV and SMoV coinfection inhibits the growth and development of 'Benihope' strawberry plants, and the plants respond to viruses by regulating stomatal aperture, the accumulation of ABA and antioxidants.To our knowledge, this study contributes information to understand how both viruses impair the strawberry growth and development for the first time.

show abstract

Section: Discussionsupporting

confidence: 92%

Effect of strawberry vein banding virus and strawberry mottle virus co-infection on the growth and development of strawberry

Fan¹,

Song²,

Khoo³

et al. 2022

Biocell

View full text Add to dashboard Cite

show abstract

“…More recently, increasing evidence has shown that catalase plays a vital role in plant resistance to diverse pathogens. For instance, Nicotiana tabacum with CAT1 overexpression inhibited chilli veinal mottle virus (ChiVMV) accumulation while CAT1 knockout mutant became more susceptible (Yang et al ., 2020). In addition, catalase mutant ( cat2‐2 ) was demonstrated to increase the susceptibility of Arabidopsis to the hemibiotrophic fungus Colletotrichum higginsianum (Schmidt et al ., 2020).…”

Section: Discussionmentioning

confidence: 99%

“…In view of the important roles of catalase in plant defence, it appears that this protein is a common target of various plant pathogens. Some pathogens have evolved effectors to regulate plant defence through interactions with plant CATs (Zhang et al ., 2015; Yang et al ., 2020). In this study, we demonstrated that MCMV P31 physically interacts with ZmCAT1 and ZmCAT3 and represses their catalase activity (Figs 5, 6a).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, evidence has emerged that SA accumulates and binds to AtCAT2 to inhibit its enzymatic activity upon exposure to biotrophic pathogens (Yuan et al ., 2017). Although catalases have previously been reported to be important in the interaction with viral effectors (Inaba et al ., 2011; Mathioudakis et al ., 2013; Roshan et al ., 2018; Yang et al ., 2020), how plant viruses modulate catalases to overcome host defence remains largely unexplored.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A novel pathogenicity determinant hijacks maize catalase 1 to enhance viral multiplication and infection

et al. 2021

View full text Add to dashboard Cite

Pathogens have evolved various strategies to overcome host immunity for successful infection. Maize chlorotic mottle virus (MCMV) can cause lethal necrosis in maize (Zea mays) when it coinfects with a virus in the Potyviridae family. However, the MCMV pathogenicity determinant remains largely unknown. Here we show that the P31 protein of MCMV is important for viral accumulation and essential for symptom development. Ectopic expression of P31 using foxtail mosaic virus or potato virus X induced necrosis in systemically infected maize or Nicotiana benthamiana leaves. Maize catalases (CATs) were shown to interact with P31 in yeast and in planta. P31 accumulation was elevated through its interaction with ZmCAT1. P31 attenuated the expression of salicylic acid (SA)-responsive pathogenesis-related (PR) genes by inhibiting catalase activity during MCMV infection. In addition, silencing of ZmCATs using a brome mosaic virus-based gene silencing vector facilitated MCMV RNA and coat protein accumulation. This study reveals an important role for MCMV P31 in counteracting host defence and inducing systemic chlorosis and necrosis. Our results have implications for understanding the mechanisms in defence and counter-defence during infection of plants by various pathogens.

show abstract

“…HCPro is a well-known multifunctional protease that helps in aphid transmission and viral particle formation, acting as an RSS at least in potyviruses and rymoviruses [24]. It is a protein extensively studied, and the list of identified interacting host factors continuously grows [4,[24][25][26]. Type B P1 proteins are less characterized.…”

Section: Introductionmentioning

confidence: 99%

An Importin-β-like Protein from Nicotiana benthamiana Interacts with the RNA Silencing Suppressor P1b of the Cucumber Vein Yellowing Virus, Modulating Its Activity

García

Bedoya

Garcı́a

et al. 2021

Viruses

View full text Add to dashboard Cite

During a plant viral infection, host–pathogen interactions are critical for successful replication and propagation of the virus through the plant. RNA silencing suppressors (RSSs) are key players of this interplay, and they often interact with different host proteins, developing multiple functions. In the Potyviridae family, viruses produce two main RSSs, HCPro and type B P1 proteins. We focused our efforts on the less known P1b of cucumber vein yellowing virus (CVYV), a type B P1 protein, to try to identify possible factors that could play a relevant role during viral infection. We used a chimeric expression system based on plum pox virus (PPV) encoding a tagged CVYV P1b in place of the canonical HCPro. We used that tag to purify P1b in Nicotiana-benthamiana-infected plants and identified by mass spectrometry an importin-β-like protein similar to importin 7 of Arabidopsis thaliana. We further confirmed the interaction by bimolecular fluorescence complementation assays and defined its nuclear localization in the cell. Further analyses showed a possible role of this N. benthamiana homolog of Importin 7 as a modulator of the RNA silencing suppression activity of P1b.

show abstract

Chilli veinal mottle virus HCPro interacts with catalase to facilitate virus infection in Nicotiana tabacum

Cited by 41 publications

References 56 publications

Effect of strawberry vein banding virus and strawberry mottle virus co-infection on the growth and development of strawberry

Effect of strawberry vein banding virus and strawberry mottle virus co-infection on the growth and development of strawberry

A novel pathogenicity determinant hijacks maize catalase 1 to enhance viral multiplication and infection

An Importin-β-like Protein from Nicotiana benthamiana Interacts with the RNA Silencing Suppressor P1b of the Cucumber Vein Yellowing Virus, Modulating Its Activity

Contact Info

Product

Resources

About