Children with peanut allergy recognize predominantly Ara h2 and Ara h6, which remains stable over time

Flinterman, Annebeth E.; Hoffen, Els van; Jäger, Cornelie; Koppelman, Stef J.; Pasmans, Suzanne G.M.A.; Hoekstra, M. O.; Bruijnzeel-Koomen, Carla A.F.M.; Knulst, André C.; Knol, Edward F.

doi:10.1111/j.1365-2222.2007.02764.x

Cited by 128 publications

(139 citation statements)

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“…However, we did not find any differences for anaphylaxis or urticaria/angioedema. Our data agree with previous studies (28,47), although one study has shown anaphylaxis to be more highly associated with Ara h 2 (48).…”

Section: Discussionsupporting

confidence: 93%

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Influence of age on IgE response in peanut‐allergic children and adolescents from the Mediterranean area

García-Blanca

Aranda

Blanca‐López

et al. 2015

Pediatric Allergy Immunology

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Background Peanut allergens are common triggers of food allergy. Analyses of sensitization patterns, relationships with other allergens, clinical symptoms, and variation with age are needed. We studied sensitization to Ara h 2, Ara h 9, and Pru p 3 in a peanut allergic children/adolescents and the relationship with peach and pollen. Methods Peanut allergic patients aged between 1 and 20 years old were classified into two groups: A) allergic to peanut only and B) allergic to peach and peanut. The IgE response was measured to Ara h 2, Ara h 9, and Pru p 3. Results Of 964 subjects evaluated, 28% were allergic to peanut. From this group, 68% were also sensitized to pollen. Urticaria was the most frequent entity followed by anaphylaxis and OAS. Fifty‐eight percent had Ara h 2‐ and/or Ara h 9‐specific IgE. More than half reported symptoms with peanut alone (Group A) and 35% to peanut and peach (Group B). We observed significant differences in sex, age, onset of symptoms, and sensitization to Artemisia between groups. IgE response to Ara h 2 was more frequent in Group A, and Ara h 9 and Pru p 3 in Group B. We observed a decrease in sensitization to Ara h 2 and an increase to Ara h 9 and Pru p 3 with increasing age. Conclusion Peanut allergy is frequent in subjects with allergy to plant foods, with Ara h 2 and Ara h 9 being two important allergens. In younger patients, Ara h 2 predominates over Ara h 9. The reverse was observed in older patients.

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Section: Discussionsupporting

confidence: 93%

“…In Group B, Ara h 9 and Pru p 3 were significantly higher. These findings agree with other studies (17,18,45,47,50). However, only 22% of cases from Group A had systemic reactions (anaphylaxis and urticaria/angioedema) and only 12% of Ara h 2 positive cases Ara h 2 from Group B had systemic reactions.…”

Section: Discussionsupporting

confidence: 92%

Influence of age on IgE response in peanut‐allergic children and adolescents from the Mediterranean area

García-Blanca

Aranda

Blanca‐López

et al. 2015

Pediatric Allergy Immunology

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“…Ara h 1, Ara h 2, and Ara h 3 are considered the major peanut allergens (Burks et al, 1998), as well as Ara h 6 in some studies (Flinterman et al, 2007).…”

Section: Identified Allergensmentioning

confidence: 99%

Scientific Opinion on the evaluation of allergenic foods and food ingredients for labelling purposes

Products¹

2014

EFS2

115

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Following a request from the Food Safety Authority of Ireland, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA Panel) was asked to deliver a scientific opinion on the evaluation of allergenic foods and food ingredients for labelling purposes. In view of the request, the NDA Panel decided to update its previous opinions relative to food ingredients or substances with known allergenic potential listed in Annex IIIa of 2003/89/EC, as amended. These include cereals containing gluten, milk and dairy products, eggs, nuts, peanuts, soy, fish, crustaceans, molluscs, celery, lupin, sesame, mustard and sulphites. The opinion relates to immunoglobulin (Ig)E-and non-IgE-mediated food allergy, to coeliac disease and to adverse reactions to sulphites in food, and it does not address non-immune-mediated adverse reactions to food. It includes information on the prevalence of food allergy in unselected populations, proteins identified as food allergens, cross-reactivities, the effects of food processing on the allergenicity of foods and ingredients, methods for the detection of allergens and allergenic foods, doses observed to trigger adverse reactions in sensitive individuals and risk assessment methodologies that have been used to derive individual and population thresholds for selected allergenic foods. The present opinion relates to immunoglobulin (Ig)E-and non-IgE-mediated food allergy, to coeliac disease and to adverse reactions to sulphites in food, and it does not address non-immune-mediated adverse reactions to food. For each food ingredient or substance listed in Annex IIIa, it includes information on the prevalence of food allergy in unselected populations, on proteins identified as food allergens, on cross-reactivities, on the effects of food processing on the allergenicity of foods and ingredients, on methods for the detection of allergens and allergenic foods, and on doses observed to trigger adverse reactions in sensitive individuals.Immune-mediated adverse reactions to foods manifest with clinical signs and symptoms of variable severity and duration, which may affect different organs and systems. Anaphylactic reactions to food are IgE mediated and may occur at any age. Non-IgE-mediated food allergy includes a wide range of diseases, including protein-induced enterocolitis and eosinophilic oesophagitis.A careful family and clinical history are the basis for diagnosis of food allergy. Food diaries, skin prick tests (SPTs), allergen-specific IgE measurements, food elimination diets and food challenges are part of the standard protocol for the diagnosis of food allergy. A positive SPT indicates sensitisation to the tested food, but it is not diagnostic of food allergy. Allergen-specific serum IgE antibodies similarly denote sensitisation to a particular food, but they are not diagnostic without a clinical history or food challenge. The use of atopy patch tests for the diagnosis of food allergy is controversial. Other available tests have no current role in the diagnosis of food allergy. Diag...

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“…Although the protein Ara h 1 (vicilin family) accounts for about 20% of the total peanut proteins, Ara h 2 (conglutin family) constitutes the most frequently recognized allergen in children (Nicolaou et al, 2005;Flinterman et al, 2007). In the present work, an electrochemical genosensor for detection of an 86-mer DNA sequence encoding part of the allergenic protein Ara h 2 from peanut, was developed.…”

Section: Introductionmentioning

confidence: 97%

Development of a genosensor for peanut allergen ARA h 2 detection and its optimization by surface response methodology

López

Cabanillas

Castañón

et al. 2014

Biosensors and Bioelectronics

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. bealopru@ucm.es AbstractA new selective electrochemical genosensor has been developed for the detection of an 86-mer DNA peanut sequence encoding part of the allergen Ara h 2 (conglutinhomologue protein). The method is based on a sandwich format, which presents two advantages: it permits shortening the capture probe and avoids labeling of the target.Screen-printed gold electrodes have been used as platform for the immobilization of oligonucleotides by the well-known S-Au bond. Mixed self-assembled monolayers (SAM), including thiol-modified capture probe and mercaptohexanol, were prepared to achieve an organized, homogeneous and not too compact SAM in which unspecific adsorption of the capture probe would be prevented. The optimization of the sensing phase was carried out using the Design of Experiments (DoE) approach.Traditionally, response optimization is achieved by changing the value of one factor at a time until there is no further improvement. However, DoE involves regulating the important factors so that the result becomes optimal. Optimized conditions were found to be 1.34 µM for capture probe concentration and 3.15 mM for 2 mercaptohexanol (spacer) concentration. When the optimal conditions were employed the analytical performance of the proposed genosensor improved significantly, showing a sensitivity as high as 3 µA/nM, with a linear range from 5 10 -11 to 5 10 -8 M and a detection limit of 10 pM.

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Children with peanut allergy recognize predominantly Ara h2 and Ara h6, which remains stable over time

Abstract: Ara h2 and Ara h6 are the most frequently recognized major peanut allergens in children. The individual reactivity to the major peanut allergens remained stable over time, despite DBPCFC.

Cited by 128 publications

References 26 publications

Influence of age on IgE response in peanut‐allergic children and adolescents from the Mediterranean area

Influence of age on IgE response in peanut‐allergic children and adolescents from the Mediterranean area

Scientific Opinion on the evaluation of allergenic foods and food ingredients for labelling purposes

Development of a genosensor for peanut allergen ARA h 2 detection and its optimization by surface response methodology

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