Chicken serum uric acid level is regulated by glucose transporter 9

Abstract

Objective: Glucose transporter 9 (GLUT9) is a uric acid transporter that associated with uric absorption in mice and human; but it is unknown whether GLUT9 involves in chicken uric acid regulation. This experiment aimed to investigate the chicken GLUT9 expression and serum uric acid (SUA) level. Methods: Sixty chickens were divided into 4 groups (n = 15): a control group (NC); a sulfonamide-treated group (SD) supplemented with sulfamonomethoxine sodium via drinking water (8 mg/L); a fishmeal group (FM) supplemented with 16% fishmeal in diet; and a uric acid-injection group (IU), where UA (250 mg/kg) was intraperitoneally injected once a day. The serum was collected weekly to detect the SUA level. Liver, kidney, jejunum and ileum tissues were collected to detect the GLUT9 mRNA and protein expression. Results: The results showed in the SD and IU groups, the SUA level increased and GLUT9 expression increased in the liver, but decreased in the kidney, jejunum and ileum. In the FM group, the SUA level decreased slightly and GLUT9 expression increased in the kidney, but decreased in the liver, jejunum and ileum. Correlation analysis revealed that liver GLUT9 expression correlated positively and renal GLUT9 expression correlated negatively with the SUA level. Conclusion: These results demonstrate that there maybe a feedback regulation of GLUT9 in the chicken liver and kidney to maintain the SUA balance; however, the underlying mechanism needs to be investigated in future studies.

Effects of Urtica urens in the Feed of Broilers on Performances, Digestibility, Carcass Characteristics and Blood Parameters

“…Uric acid is synthesized in the liver [ 48 ], and significant increases in uric acid levels are indicative of nephrotoxicity in broiler chickens [ 49 ], whose mean values are between 25 to 45 g/L [ 50 ]. Our results found no differences between treatments, perhaps because uric acid production depends on dietary protein intake and the breakdown of endogenous purines through xanthine oxidase [ 51 ].…”

Ameliorative action of “daitongxiao” against hyperuricemia includes the “uric acid transporter group”

“…The first mechanism is the excessive production of UA, which is mainly caused due to metabolic processes, although food intake plays a minor role ( Yang et al, 2022 ). For example, the intake of purine-rich foods, such as broth, seafood, and beer, increases UA load in the body and exacerbates HUA as purine is the precursor of UA ( Ding et al, 2021 ). Anabolic dysregulation accounts for disease onset in approximately 20% of patients ( Pang et al, 2021 ).…”

The improvement of meat nutrient composition in broiler chickens fed diets containing rice bran tempeh supplemented with cellulose

“…The decrease in the pH of the diets is thought to be a factor that lowers the blood pH in the present study. Uric acid concentration is in part regulated by blood glucose (Ding et al, 2021). Therefore, not decreasing blood glucose is one of the causes of not decreasing blood uric acid.…”