Chicken miR-126-5p negatively regulates antiviral innate immunity by targeting TRAF3

Wang, Jie; Cheng, Yuqiang; Wang, Longlong; Sun, Aixi; Lin, Zhenyu; Zhu, Wenhan; Wang, Zhaofei; Ma, Jingjiao; Wang, Henan; Yan, Yaxian; Sun, Jianhe

doi:10.1186/s13567-022-01098-x

Cited by 9 publications

(6 citation statements)

References 54 publications

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“…miR-126 notably promoted NDV replication, suggesting that it regulates the host’s immune response by suppressing the ‘cytokine storm’ after viral infection. This negative regulation effectively prevents damage to the host caused by an excessive immune response [ 48 ]. Ishizaki et al have demonstrated that miR-126 regulates ERK signaling and cytokine production by directly targeting Spred1 (Sprouty-related Ena/VASP homology-1 domain-containing protein).…”

Section: Discussionmentioning

confidence: 99%

miRNAs as Biomolecular Markers for Food Safety, Quality, and Traceability in Poultry Meat—A Preliminary Study

Baraldo,

Buzzoni,

Pasti

et al. 2024

Molecules

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In this study, the expression and abundance of two candidate chicken (Gallus gallus; gga) microRNAs (miRNAs, miR), gga-miR-21-5p (miR-21) and gga-miR-126-5p (miR-126), have been analyzed in order to identify biomarkers for the traceability and quality of poultry meat. Two breeds of broiler chickens were tested: the most common Ross308 (fast-growing) and the high-quality Ranger Gold (slow-growing). A preliminary analysis of the two miRNAs expressions was conducted across various tissues (liver, lung, spleen, skeletal muscle, and kidney), and the three tissues (lung, spleen, and muscle) with a higher expression were chosen for further analysis. Using quantitative reverse transcription polymerase chain reaction (RT-qPCR), the expression of miRNAs in the three tissues of a total of thirteen animals was determined. The results indicate that miR-126 could be a promising biomarker for the lung tissue in the Ranger Gold (RG) breed (p < 0.01), thus suggesting a potential applicability for tracing hybrids. RG exhibits a significantly higher miR-126 expression in the lung tissue compared to the Ross308 broilers (R308), an indication of greater respiratory capacity and, consequently, a higher oxidative metabolism of the fast-growing hybrid. During sampling, two R308 broilers presented some anomalies, including airsacculitis, hepatic steatosis, and enlarged spleen. The expression of miR-126 and miR-21 was compared in healthy animals and in those presenting anomalies. Chickens with airsacculitis and hepatic steatosis showed an up-regulation of miR-21 and miR-126 in the most commercially valuable tissue, the skeletal muscle or breast (p < 0.05).

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Section: Discussionmentioning

confidence: 99%

miRNAs as Biomolecular Markers for Food Safety, Quality, and Traceability in Poultry Meat—A Preliminary Study

Baraldo,

Buzzoni,

Pasti

et al. 2024

Molecules

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“…Numerous studies have shown that miRNAs are involved in the regulation of host cytokine storms upon pathogen invasion. MiR-223 attenuates acute inflammation in the lungs of mice caused by Streptococcus agalactiae [ 28 ]; inhibition of miRNA-34a promotes M2 macrophage polarization and ameliorates LPS-induced lung injury by targeting Kruppel-like factor 4 (KLF4) [ 29 ]; and miR-126-5p regulates host innate immune responses by suppressing the host inflammatory factor storm [ 30 ]. In the present study, we found that let-7d was able to suppress the MG-triggered cytokine storm to regulate the host immune response (Figure 1 E).…”

Section: Discussionmentioning

confidence: 99%

Low let-7d microRNA levels in chick embryos enhance innate immunity against Mycoplasma gallisepticum by suppressing the mitogen-activated protein kinase pathway

Wang

Sun

Zhao

et al. 2023

Vet Res

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Chick embryos are a valuable model for studying immunity and vaccines. Therefore, it is crucial to investigate the molecular mechanism of the Mycoplasma gallisepticum (MG)-induced immune response in chick embryos for the prevention and control of MG. In this study, we screened for downregulated let-7d microRNA in MG-infected chicken embryonic lungs to explore its involvement in the innate immune mechanism against MG. Here, we demonstrated that low levels of let-7d are a protective mechanism for chicken embryo primary type II pneumocytes (CP-II) in the presence of MG. Specifically, we found that depressed levels of let-7 in CP-II cells reduced the adhesion capacity of MG. This suppressive effect was achieved through the activated mitogen-activated protein kinase phosphatase 1 (MKP1) target gene and the inactivated mitogen-activated protein kinase (MAPK) pathway. Furthermore, MG-induced hyperinflammation and cell death were both alleviated by downregulation of let-7d. In conclusion, chick embryos protect themselves against MG infection through the innate immune molecule let-7d, which may result from its function as an inhibitor of the MAPK pathway to effectively mitigate MG adhesion, the inflammatory response and cell apoptosis. This study may provide new insight into the development of vaccines against MG.

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“…Previous studies suggest that DUSP10 (MKP5) inactivate cytokines and pro-inflammatory cytokines by inactivating MAP kinases to achieve equilibrium [ 39 , 41 ]. The other target gene, TRAF3 , encodes a protein that activates the secretion of type I IFNs, such as IFN-α and IFN-β [ 42 ]. After infection with the avian influenza virus, TRAF3 interacts with Mitochondrial antiviral signaling protein (MAVS), which is associated with retinoic acid-inducible gene I (RIG-I) signaling against virus infection [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA expression profiling in the lungs of genetically different Ri chicken lines against the highly pathogenic avian influenza H5N1 virus

Lee¹,

Kang²,

Heo³

et al. 2023

J Anim Sci Technol

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The highly pathogenic avian influenza (HPAI) virus triggers infectious diseases, resulting in pulmonary damage and high mortality in domestic poultry worldwide. This study aimed to analyze miRNA expression profiles after infection with the HPAI H5N1 virus in resistant and susceptible lines of Ri chickens.For this purpose, resistant and susceptible lines of Vietnamese Ri chicken were used based on the A/G allele of Mx and BF2 genes. These genes are responsible for innate antiviral activity and were selected to determine differentially expressed (DE) miRNAs in HPAI-infected chicken lines using small RNA sequencing.A total of 44 miRNAs were differentially expressed after 3 days of infection with the H5N1 virus.Computational program analysis indicated the candidate target genes for differentially expressed (DE) miRNAs to possess significant functions related to cytokines, chemokines, MAPK signaling pathway, ErBb signaling pathway, and Wnt signaling pathway. Several DE miRNA-mRNA matches were suggested to play crucial roles in mediating immune functions against viral evasion. These results revealed the potential regulatory roles of miRNAs in the immune response of the two Ri chicken lines against HPAI H5N1 virus infection in the lungs.

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Chicken miR-126-5p negatively regulates antiviral innate immunity by targeting TRAF3

Cited by 9 publications

References 54 publications

miRNAs as Biomolecular Markers for Food Safety, Quality, and Traceability in Poultry Meat—A Preliminary Study

miRNAs as Biomolecular Markers for Food Safety, Quality, and Traceability in Poultry Meat—A Preliminary Study

Low let-7d microRNA levels in chick embryos enhance innate immunity against Mycoplasma gallisepticum by suppressing the mitogen-activated protein kinase pathway

MicroRNA expression profiling in the lungs of genetically different Ri chicken lines against the highly pathogenic avian influenza H5N1 virus

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