Chicken line-dependent mortality after experimental infection with three type IIxIII recombinant Toxoplasma gondii clones

Schares, Gereon; Herrmann, Daland; Maksimov, Pavlo; Matzkeit, Beate; Conraths, Franz J.; Moré, Gastón; Preisinger, Rudolf; Weigend, Steffen

doi:10.1016/j.exppara.2016.11.004

Cited by 12 publications

(6 citation statements)

References 61 publications

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“…Effect of breed/strain of chickens, T. gondii genotype on toxoplasmosis in chickens Breed or strain of chicken can influence the course of T. gondii infection (Schares et al, 2017b). One-day-old chickens of two lines (white layer, line A, brown layer, line B) inoculated intravenously with tachyzoites of a cross line of Type II/Type III T. gondii strain; higher mortality was observed in line A chickens (Schares et al, 2017b). Serum antibody levels assessed by SAG1-ELISA at 31 days p.i.…”

Section: Serotypingmentioning

confidence: 99%

Epidemiologic significance ofToxoplasma gondiiinfections in chickens (Gallus domesticus): the past decade

Dubey¹,

Pena²,

Cerqueira‐Cézar³

et al. 2020

Parasitology

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Toxoplasma gondii infections are common in humans and animals worldwide. Domestic free-range chickens (Gallus domesticus) are excellent sentinels of environmental contamination with T. gondii oocysts because they feed on the ground. Chickens can be easily infected with T. gondii; however, clinical toxoplasmosis is rare in these hosts. Chickens are comparatively inexpensive and thus are good sentinel animals for T. gondii infections on the farms. Here, the authors reviewed prevalence, the persistence of infection, clinical disease, epidemiology and genetic diversity of T. gondii strains isolated from chickens worldwide for the past decade. Data on phenotypic and molecular characteristics of 794 viable T. gondii strains from chickens are discussed, including new data on T. gondii isolates from chickens in Brazil. This paper will be of interest to biologists, epidemiologists, veterinarians and parasitologists.

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Section: Serotypingmentioning

confidence: 99%

Epidemiologic significance ofToxoplasma gondiiinfections in chickens (Gallus domesticus): the past decade

Dubey¹,

Pena²,

Cerqueira‐Cézar³

et al. 2020

Parasitology

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“…MC-qPCR was essentially performed as described [ 29 ] with some slight modifications [ 22 ]. For the PD-qPCR, tissues were digested [ 11 , 30 ] and the qPCR performed on digests as described [ 31 , 32 ] using primers and a probe targeting the 529 bp repeat of T. gondii [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens

et al. 2020

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Background: Free-ranging chickens are often infected with Toxoplasma gondii and seroconvert upon infection. This indicates environmental contamination with T. gondii. Methods: Here, we established a bead-based multiplex assay (BBMA) using the Luminex technology for the detection of T. gondii infections in chickens. Recombinant biotinylated T. gondii surface antigen 1 (TgSAG1 bio) bound to streptavidin-conjugated magnetic Luminex beads served as antigen. Serum antibodies were detected by a fluorophore-coupled secondary antibody. Beads of differing color codes were conjugated with anti-chicken IgY or chicken serum albumin and served for each sample as an internal positive or negative control, respectively. The assay was validated with sera from experimentally and naturally infected chickens. The results were compared to those from reference methods, including other serological tests, PCRs and bioassay in mice. Results: In experimentally infected chickens, the vast majority (98.5%, n = 65/66) of birds tested seropositive in the BBMA. This included all chickens positive by magnetic-capture PCR (100%, n = 45/45). Most, but not all inoculated and TgSAG1 bio-BBMA-positive chickens were also positive in two previously established TgSAG1-ELISAs (TgSAG1-ELISA SL , n = 61/65; or TgSAG1-ELISA SH , n = 60/65), or positive in an immunofluorescence assay (IFAT, n = 64/65) and in a modified agglutination test (MAT, n = 61/65). All non-inoculated control animals (n = 28/28, 100%) tested negative. In naturally exposed chickens, the TgSAG1 bio-BBMA showed a high sensitivity (98.5%; 95% confidence interval, CI: 90.7-99.9%) and specificity (100%; 95% CI: 85.0-100%) relative to a reference standard established using ELISA, IFAT and MAT. Almost all naturally exposed chickens that were positive in bioassay or by PCR tested positive in the TgSAG1 bio-BBMA (93.5%; 95% CI: 77.1-98.9%), while all bioassay-or PCR-negative chickens remained negative (100%; 95% CI: 85.0-100%). Conclusions: The TgSAG1 bio-BBMA represents a suitable method for the detection of T. gondii infections in chickens with high sensitivity and specificity, which is comparable or even superior to other tests. Since assays based on this methodology allow for the simultaneous analysis of a single biological sample with respect to multiple analytes, the described assay may represent a component in future multiplex assays for broad serological monitoring of poultry and other farm animals for various pathogens.

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“…MC-qPCR was essentially performed as described [29] with some slight modi cations [22]. For the PD-qPCR, tissues were digested [11,30] and the qPCR performed on digests as described [31,32] using primers and a probe targeting the 529 bp repeat of T. gondii [33].…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens

Fabian

Hedar

Koethe

et al. 2020

Preprint

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Background: Free-ranging chickens are often infected with Toxoplasma gondii and seroconvert upon infection. This indicates environmental contamination with T. gondii. Methods: Here, we established a bead-based multiplex assay (BBMA) using the Luminex technology for the detection of T. gondii infections in chickens. Recombinant biotinylated T. gondii surface antigen 1 (TgSAG1bio) bound to streptavidin-conjugated magnetic Luminex beads served as antigen. Serum antibodies were detected by a fluorophore-coupled secondary antibody. Beads of differing color codes were conjugated with anti-chicken IgY or chicken serum albumin and served for each sample as an internal positive or negative control, respectively. The assay was validated with sera from experimentally and naturally infected chickens. The results were compared to those from reference methods, including other serological tests, PCRs and bioassay in mice.Results: In experimentally infected chickens, the vast majority (98.5%, n = 65/66) of birds tested seropositive in the BBMA. This included all chickens positive by magnetic-capture PCR (100%, n = 45/45). Most, but not all inoculated and TgSAG1bio-BBMA-positive chickens were also positive in two previously established TgSAG1-ELISAs (TgSAG1-ELISASL, n = 61/65; or TgSAG1-ELISASH, n = 60/65), or positive in an immunofluorescence assay (IFAT, n = 64/65)) and in a modified agglutination test (MAT, n = 61/65). All non-inoculated control animals (n = 28/28, 100%) tested negative. In naturally exposed chickens, the TgSAG1bio-BBMA showed a high sensitivity (98.5%; 95% confidence interval, CI: 90.7–99.9%) and specificity (100%; 95% CI: 85.0–100%) relative to a reference standard established using ELISA, IFAT and MAT. Almost all naturally exposed chickens that were positive in bioassay or by PCR tested positive in the TgSAG1bio-BBMA (93.5%; 95% CI: 77.1–98.9%), while all bioassay- or PCR-negative chickens remained negative (100%; 95% CI: 85.0–100%).Conclusions: The TgSAG1bio-BBMA represents a suitable method for the detection of T. gondii infections in chickens with high sensitivity and specificity, which is comparable or even superior to other tests. Since assays based on this methodology allow for the simultaneous analysis of a single biological sample with respect to multiple analytes, the described assay may represent a component in future multiplex assays for broad serological monitoring of poultry and other farm animals for various pathogens.

show abstract

Chicken line-dependent mortality after experimental infection with three type IIxIII recombinant Toxoplasma gondii clones

Cited by 12 publications

References 61 publications

Epidemiologic significance ofToxoplasma gondiiinfections in chickens (Gallus domesticus): the past decade

Epidemiologic significance ofToxoplasma gondiiinfections in chickens (Gallus domesticus): the past decade

Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens

Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens

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