Chicken intestinal microbiota modulation of resistance to nephropathogenic infectious bronchitis virus infection through IFN-I

Yin, Huan; Liu, Zhendong; Zhang, Weiwei; Yang, Qingzhu; Yu, Tao; Jiang, Xinjie

doi:10.1186/s40168-022-01348-2

Cited by 7 publications

(7 citation statements)

References 57 publications

(75 reference statements)

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“…It has also previously been observed that depletion of the microbiota leads to an impaired immune response to nephropathogenic IBV (76). In our study, large, genotype-independent differences in the composition of the microbiota were observed in IBV vaccinated vs. unvaccinated birds.…”

Section: Discussionmentioning

confidence: 86%

“…It is also possible that exposing chickens to different microbial sources early in life may lead to differences in the relationship between cDCs and the microbiota, as the gut microbiota plays an important role in shaping the chicken immune system (2), with early-life antibiotic exposure; exposure to adult caecal microbes and raising environment (cage vs. litter floor pens) all impacting the maturation of the chicken immune system (4,74,75). It has also previously been observed that depletion of the microbiota leads to an impaired immune response to nephropathogenic IBV (76). In our study, large, genotype-independent differences in the composition of the microbiota were observed in IBV vaccinated vs. unvaccinated birds.…”

Section: Discussionmentioning

confidence: 99%

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Infectious bronchitis virus vaccination, but not the presence of XCR1, is correlated with large differences in chicken caecal microbiota

Glendinning,

Wu,

Vervelde

et al. 2024

Preprint

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The chicken immune system and microbiota play vital roles in maintaining gut homeostasis and protecting against pathogens. In mammals, XCR1+ conventional dendritic cells (cDCs) are located in the gut-draining lymph nodes and play a major role in gut homeostasis. These cDCs sample antigens in the gut luminal contents and limit the inflammatory response to gut commensal microbes by generating appropriate regulatory and effector T-cell responses. We hypothesised that these cells play similar roles in sustaining gut homeostasis in chickens, and that chickens lacking XCR1 were likely to contain a dysbiotic caecal microbiota. Here we compare the caecal microbiota of chickens that were either heterozygous or homozygous XCR1 knockouts, that had or had not been vaccinated for infectious bronchitis virus (IBV). We used short-read (Illumina) and long-read (PacBio HiFi) metagenomic sequencing to reconstruct 670 high-quality, strain-level metagenome assembled genomes. We found no significant differences between alpha-diversity or the abundance of specific microbial taxa between genotypes. However, IBV vaccination was found to correlate with significant differences in the richness and beta-diversity of the microbiota, and to the abundance of forty bacterial genera. In conclusion, we found that a lack of XCR1 was not correlated with significant changes in the chicken microbiota, but IBV vaccination was.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Infectious bronchitis virus vaccination, but not the presence of XCR1, is correlated with large differences in chicken caecal microbiota

Glendinning,

Wu,

Vervelde

et al. 2024

Preprint

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“…Previous studies have consistently indicated that the administration of levofloxacin can disturb the equilibrium of the intestinal microbiota, thereby inducing antibiotic-associated inflammation within the gastrointestinal tract [ 55 ]. Moreover, previous research conducted by our team and other scholars has provided evidence that the depletion of the intestinal microbiota resulting from the administration of antimicrobial drugs substantially elevates the likelihood of bacterial infectious diseases [ 53 , 54 , 72 – 74 ]. This correlation is closely linked to the reduction of immunoregulatory metabolites derived from the intestinal microbiota.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of levofloxacin-loaded porcine acellular dermal matrix hydrogel and functional assessment in urinary tract infection

Yang,

et al. 2024

J Nanobiotechnol

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Bacterial cystitis, a commonly occurring urinary tract infection (UTI), is renowned for its extensive prevalence and tendency to recur. Despite the extensive utilization of levofloxacin as a conventional therapeutic approach for bacterial cystitis, its effectiveness is impeded by adverse toxic effects, drug resistance concerns, and its influence on the gut microbiota. This study introduces Lev@PADM, a hydrogel with antibacterial properties that demonstrates efficacy in the treatment of bacterial cystitis. Lev@PADM is produced by combining levofloxacin with decellularized porcine acellular dermal matrix hydrogel and exhibits remarkable biocompatibility. Lev@PADM demonstrates excellent stability as a hydrogel at body temperature, enabling direct administration to the site of infection through intravesical injection. This localized delivery route circumvents the systemic circulation of levofloxacin, resulting in a swift and substantial elevation of the antimicrobial agent’s concentration specifically at the site of infection. The in vivo experimental findings provide evidence that Lev@PADM effectively prolongs the duration of levofloxacin’s action, impedes the retention and invasion of E.coli in the urinary tract, diminishes the infiltration of innate immune cells into infected tissues, and simultaneously preserves the composition of the intestinal microbiota. These results indicate that, in comparison to the exclusive administration of levofloxacin, Lev@PADM offers notable benefits in terms of preserving the integrity of the bladder epithelial barrier and suppressing the recurrence of urinary tract infections. Graphical Abstract

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“…Till now, studies about the influence of intestinal microbiota on viral infection are mainly conducted in mammalian model. Recent work demonstrated that intestinal microbiota improved resistance of chicken against nephropathogenic infectious bronchitis virus ( 12 ), suggesting a conserved role of microbiota in regulating viral infection. However, the influence of commensal bacteria on viral infection in lower vertebrates is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

The intestinal microbiome and Cetobacterium somerae inhibit viral infection through TLR2-type I IFN signaling axis in zebrafish

Liang,

Li,

Chen

et al. 2024

Preprint

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Accumulated evidences demonstrate that intestinal microbiome can inhibit viral infection. However, our knowledge of the signaling pathways and specific commensal microbes that mediate the antiviral response is limited. Here, a rhabdoviral infection model in zebrafish allows us to investigate the modes of action of microbiome-mediated antiviral effect. We observed that antibiotics-treated and germ-free zebrafish exhibited greater viral infection. Mechanistically, depletion of the intestinal microbiome alters TLR2-Myd88 signaling, and blunts neutrophil response and type I interferon (IFN) production. Moreover, a single commensal bacterium, Cetobacterium somerae, recapitulated TLR2- and type I IFN-dependent antiviral effect of the microbiome in gnotobiotic zebrafish, and C. somerae exopolysaccharides (CsEPS) was the effector molecule that engaged TLR2 to mediate antiviral function. Together, our results suggest a conserved role of intestinal microbiome in regulating type I IFN response among vertebrates, and reveal that the intestinal microbiome inhibits viral infection through a CsEPS-TLR2-type I IFN signaling axis in zebrafish.

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Chicken intestinal microbiota modulation of resistance to nephropathogenic infectious bronchitis virus infection through IFN-I

Cited by 7 publications

References 57 publications

Infectious bronchitis virus vaccination, but not the presence of XCR1, is correlated with large differences in chicken caecal microbiota

Infectious bronchitis virus vaccination, but not the presence of XCR1, is correlated with large differences in chicken caecal microbiota

Fabrication of levofloxacin-loaded porcine acellular dermal matrix hydrogel and functional assessment in urinary tract infection

The intestinal microbiome and Cetobacterium somerae inhibit viral infection through TLR2-type I IFN signaling axis in zebrafish

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