Chicken interferon regulatory factor 7 (IRF7) can control ALV-J virus infection by triggering type I interferon production through affecting genes related with innate immune signaling pathway

Wang, Yan; Yang, Feng; Yin, Huadong; He, Qijian; Lu, Yong; Zhu, Qing; Lan, Xi; Zhao, Xiaoling; Li, Diyan; Liu, Yiping; Xu, Hengyong

doi:10.1016/j.dci.2021.104026

Cited by 15 publications

(8 citation statements)

References 42 publications

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“…To evaluate whether organoids containing neural-like cells can use the innate immune response of chicken in the neural cell, we expose the poly:IC. Poly:IC is an analog of viral RNA; this analog has been used in many studies to stimulate the RLR and Toll-like receptor (TLR) pathways [ 20 , 22 – 25 ]. MDA5 , LGP2 , and TLR3 are important sensors for virus recognition to stimulate innate immune responses in chickens [ 23 , 26 ].…”

Section: Resultsmentioning

confidence: 99%

Organoids containing neural-like cells derived from chicken iPSCs respond to poly:IC through the RLR family

et al. 2023

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There is still much room for development in pluripotent stem cell research on avian species compared to human stem cell studies. Neural cells are useful for the evaluation of risk assessment of infectious diseases since several avian species die of encephalitis derived from infectious diseases. In this study, we attempted to develop induced pluripotent stem cells (iPSCs) technology for avian species by forming organoids containing neural-like cells. In our previous study, we established two types iPSCs from chicken somatic cells, the first is iPSCs with PB-R6F reprogramming vector and the second is iPSCs with PB-TAD-7F reprogramming vector. In this study, we first compared the nature of these two cell types using RNA-seq analysis. The total gene expression of iPSCs with PB-TAD-7F was closer to that of chicken ESCs than that of iPSCs with PB-R6F; therefore, we used iPSCs with PB-TAD-7F to form organoids containing neural-like cells. We successfully established organoids containing neural-like cells from iPSCs using PB-TAD-7F. Furthermore, our organoids responded to poly:IC through the RIG-I-like receptor (RLR) family. In this study, we developed iPSCs technology for avian species via organoid formation. In the future, organoids containing neural-like cells from avian iPSCs can develop as a new evaluation tool for infectious disease risk in avian species, including endangered avian species.

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Section: Resultsmentioning

confidence: 99%

Organoids containing neural-like cells derived from chicken iPSCs respond to poly:IC through the RLR family

et al. 2023

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“…The fact that chickens (and galliforms in general) do not express an IRF3 highlights the relevance of investigating regulatory mechanisms upstream its activation upon pathogen challenges. Despite the remarkable advancements made in the last decade numerous unresolved questions persist concerning IRF7 regulation of type-I IFN responses in birds (Kim et al, 2020; Wang et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Mechanisms of type I interferon production by chicken TLR21

Guabiraba,

Rodrigues,

Manna

et al. 2023

Preprint

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The innate immune response relies on the ability of host cells to rapidly detect and respond to microbial nucleic acids. Toll-like receptors (TLRs), a class of pattern recognition receptors (PRRs), play a fundamental role in distinguishing self from non-self at the molecular level. In this study, we focused on TLR21, an avian TLR that recognizes DNA motifs commonly found in bacterial genomic DNA, specifically unmethylated CpG motifs. TLR21 is believed to act as a functional homologue to mammalian TLR9. By analysing TLR21 signalling in chickens, we sought to elucidate avian TLR21 activation outputs in parallel to that of other nucleic acid species. Our analyses revealed that chicken TLR21 (chTLR21) triggers the activation of NF-kappa B and induces a potent type-I interferon response in chicken macrophages, similar to the signalling cascades observed in mammalian TLR9 activation. Notably, the transcription of interferon beta (IFNB) by chTLR21 was found to be dependent on both NF-kappa B and IRF7 signalling, but independent of the TBK1 kinase, a distinctive feature of mammalian TLR9 signalling. These findings highlight the conservation of critical signalling components and downstream responses between avian TLR21 and mammalian TLR9, despite their divergent evolutionary origins. These insights into the evolutionarily conserved mechanisms of nucleic acid sensing contribute to the broader understanding of host-pathogen interactions across species.

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“…The TLR signaling pathway is an innate immune defense, and IRF7 is a key gene in this pathway. Studies have shown that the expression levels of ISTAT1, IFN- α , and IFN- β increased after IRF7 is induced by stimulation of CEF cells, suggesting that IRF7 may be involved in the TLR signaling pathway [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Network Pharmacology-Based Strategy for Exploring the Pharmacological Mechanism of Honeysuckle (Lonicer japonica Thunb.) against Newcastle Disease

Huang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Objective. Newcastle disease causes huge economic losses in the global poultry industry. An efficient treatment is needed to deal with the variable immunogenicity of the Newcastle disease virus (NDV). This study utilized network pharmacology to study the potential therapeutic targets of Honeysuckle (Lonicer japonica Thunb.) against Newcastle disease. Methods. Venny online analysis was used to analyze the potential overlapping targets of Honeysuckle and Newcastle disease. Hub genes were obtained using the STRING database and Cytoscape 3.8.2 software. Gene Ontology (GO) functions and Kyoto Encyclopedia of Genes and Genomics (KEGG) pathway enrichment analysis using the DAVID online tool were performed on these targets. Results. Twenty-five overlapping targets were identified. The PPI network construction results included 23 nodes of 25 genes and 95 edges. It was found that the IL-6 node had the largest degree. STAT1 and IRF1, CASP9, and CASP3 had the same as well as strongest interaction strengths. GO functions, such as “cytokine activity,” had a regulatory effect on NDV. The “Toll-like receptor signaling Pathway” “Nod-like receptor signaling pathway,” “RIG-I-like receptor signaling pathway,” and “Apoptosis,” which were obtained using KEGG analysis, also indicated that these pathways can act on NDV to enhance immune function. Conclusions. In this study, the potential targets and mechanisms of action of Honeysuckle against Newcastle disease were explored through network pharmacology, which provided a theoretical basis for the treatment of Newcastle disease and provided new ideas for the development of traditional Chinese medicine for the poultry industry.

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Chicken interferon regulatory factor 7 (IRF7) can control ALV-J virus infection by triggering type I interferon production through affecting genes related with innate immune signaling pathway

Cited by 15 publications

References 42 publications

Organoids containing neural-like cells derived from chicken iPSCs respond to poly:IC through the RLR family

Organoids containing neural-like cells derived from chicken iPSCs respond to poly:IC through the RLR family

Mechanisms of type I interferon production by chicken TLR21

Network Pharmacology-Based Strategy for Exploring the Pharmacological Mechanism of Honeysuckle (Lonicer japonica Thunb.) against Newcastle Disease

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