Chicken Insulin: Radioimmunological Characterization and Enhanced Activity in Rat Fat Cells and Liver Plasma Membranes

Simon, Jean; Freychet, P; Rosselin, G

doi:10.1210/endo-95-5-1439

Cited by 95 publications

(34 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A large cross-reaction has been observed between chicken insulin and the guinea pig anti-porcine sera (Simon et al, 1974 this study is referred to as immunoreactive insulin. The sensitivity of the assay was 1 μIU ml −1 , and all of the samples were included in the same assay to avoid inter-assay variability.…”

Section: Measurement Of Plasma Insulin Levelsmentioning

confidence: 92%

Dietary fat alters the response of hypothalamic neuropeptide Y to subsequent energy intake in broiler chickens

Wang

Liu

et al. 2016

Journal of Experimental Biology

View full text Add to dashboard Cite

Dietary fat affects appetite and appetite-related peptides in birds and mammals; however, the effect of dietary fat on appetite is still unclear in chickens faced with different energy statuses. Two experiments were conducted to investigate the effects of dietary fat on food intake and hypothalamic neuropeptides in chickens subjected to two feeding states or two diets. In Experiment 1, chickens were fed a high-fat (HF) or low-fat (LF) diet for 35 days, and then subjected to fed (HF-fed, LF-fed) or fasted (HF-fasted, LF-fasted) conditions for 24 h. In Experiment 2, chickens that were fed a HF or LF diet for 35 days were fasted for 24 h and then re-fed with HF (HF-RHF, LF-RHF) or LF (HF-RLF, LF-RLF) diet for 3 h. The results showed that chickens fed a HF diet for 35 days had increased body fat deposition despite decreasing food intake even when the diet was altered during the re-feeding period (P<0.05). LF diet (35 days) promoted agouti-related peptide (AgRP) expression compared with HF diet (P<0.05) under both fed and fasted conditions. LF-RHF chickens had lower neuropeptide Y (NPY) expression compared with LF-RLF chickens; conversely, HF-RHF chickens had higher NPY expression than HF-RLF chickens (P<0.05). These results demonstrate: (1) that HF diet decreases food intake even when the subsequent diet is altered; (2) the orexigenic effect of hypothalamic AgRP; and (3) that dietary fat alters the response of hypothalamic NPY to subsequent energy intake. These findings provide a novel view of the metabolic perturbations associated with long-term dietary fat over-ingestion in chickens.

show abstract

Section: Measurement Of Plasma Insulin Levelsmentioning

confidence: 92%

Dietary fat alters the response of hypothalamic neuropeptide Y to subsequent energy intake in broiler chickens

Wang

Liu

et al. 2016

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…Plasma insulin levels were determined by radioimmunoassay as previously described by Simon et al (1974). The lowest limit of sensitivity was 2 m U/ml, and interassay variation was less than 10%.…”

Section: Histological Analysismentioning

confidence: 99%

Do age and feeding levels have comparable effects on fat deposition in breast muscle of mule ducks?

Chartrin¹,

Bernadet²,

Guy³

et al. 2007

Animal

View full text Add to dashboard Cite

The effects of age (from 1 day post-hatch to 98 days of age) and feeding levels (feed restriction followed by overfeeding v. ad libitum feeding) on lipid deposition in breast muscle (quantity and quality, localisation) of mule ducks were determined in relation to muscle energy metabolism (glycolytic and oxidative), plasma levels of lipids, glucose and insulin, and muscle capacity for lipid uptake (characterised by lipoprotein lipase (LPL) activity). Two periods were defined for age effects on intramuscular lipids in breast muscle: 21 to 42 days of age when lipids (mainly phospholipids and cholesterol provided by egg yolk) stored in the adipocytes during embryonic life were transferred to the muscle fibres and used for growth and energy requirements, 242 to 98 days of age when the muscle again stored lipids (mainly triglycerides provided by liver lipogenesis), first in fibres and then in adipocytes.Plasma glucose and insulin levels were not affected by age. Plasma levels of lipids and LPL activity in breast muscle were high at 1 and 14 days of age and then decreased, remaining stable until 98 days of age. Energy metabolism activity in the breast muscle (mainly glycolytic activity) increased with age.Feed restriction, corresponding to 79% of ad libitum intake, applied between 42 and 75 days of age only resulted in decreases in plasma insulin concentration and total lipid content of breast muscle, mainly affecting triglyceride and mono-unsaturated fatty acid (MUFA) levels. Overfeeding increased plasma levels of insulin and lipids while glycaemia remained stable. LPL activity and total lipid levels increased in breast muscle, mainly induced by deposition of triglycerides and MUFA occurring particularly during the 2nd week of this period. Glycolytic energy metabolism decreased. In response to age or feeding levels, muscle lipid levels and composition reflect plasma lipid levels and composition and high muscle lipid levels stimulate oxidative energy metabolism.

show abstract

“…It was generally suggested that the enhanced activities of [A8His]Ins [11] , chicken and turkey insulins were ascribed to the His at A8 [12][13][14] , which should account for the higher affinity for insulin receptor. Our results showed that the presence of His at A8 apparently did not enhance the receptor binding.…”

Section: Discussionmentioning

confidence: 99%

Relationship between insulin A chain regions and insulin biological activities

Yang¹

2000

WJG

View full text Add to dashboard Cite

show abstract

Chicken Insulin: Radioimmunological Characterization and Enhanced Activity in Rat Fat Cells and Liver Plasma Membranes

Cited by 95 publications

References 16 publications

Dietary fat alters the response of hypothalamic neuropeptide Y to subsequent energy intake in broiler chickens

Dietary fat alters the response of hypothalamic neuropeptide Y to subsequent energy intake in broiler chickens

Do age and feeding levels have comparable effects on fat deposition in breast muscle of mule ducks?

Relationship between insulin A chain regions and insulin biological activities

Contact Info

Product

Resources

About