Chicken<i>CCDC152</i>shares an NFYB-regulated bidirectional promoter with a<i>growth hormone receptor</i>antisense transcript and inhibits cells proliferation and migration

Lin, Shudai; Luo, Wei; Jiang, Mingya; Luo, Wen; Abdalla, Bahareldin Ali; Nie, Qinghua; Zhang, Li; Zhang, Xiquan

doi:10.18632/oncotarget.21091

Cited by 5 publications

(4 citation statements)

References 49 publications

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“…The expression levels of lnc9141 and IRX5 at different tissues or stages may be determined by the methylation levels of CpG islands on both sides of the active region. Similarly, Lin et al 37 . demonstrated that chicken CCDC152 is oriented in a head-to-head configuration with the antisense transcript of growth hormone receptor (GHR) gene.…”

Section: Discussionmentioning

confidence: 95%

lnc9141-a and -b Play a Different Role in Bovine Myoblast Proliferation, Apoptosis, and Differentiation

Zhang

Wang

et al. 2019

Molecular Therapy - Nucleic Acids

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Previously, our transcriptome sequencing revealed that lnc9141 was differentially expressed in muscles of fetal bovine, calf, and adult bovine, which is considered to provide the basis for raising the muscle mass. In this study, we identified lnc9141 characters. lnc9141 has different transcription start sites and 3′ alternative splicing sites of exon 1, producing lnc9141-a and lnc9141-b transcripts that were highly expressed in the heart and lung. Moreover, neither lnc9141-a nor lnc9141-b had the ability to encode proteins. The functions of lnc9141-a and lnc9141-b were explored by cell cycle, 5-ethynyl-2'-deoxyuridine (EdU), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed that lnc9141-a or lnc9141-b overexpression decreased the number of myoblasts in the S phase and increased the proportion of cells in the G0/G1 phase. Furthermore, overexpressing lnc9141-a and lnc9141-b respectively downregulated the expression of Cyclin D1. However, lnc9141-a or lnc9141-b interference was found to increase the number of S-phase myoblasts, and upregulate Cyclin D1 and Cyclin E expression. Through Annexin V-FITC/propidium iodide (PI) double staining and the expression of apoptosis marker genes (Bax, Bcl2, and Caspase-3), it was found that lnc9141-b could regulate the expression of Bax gene. Meantime, high expression of lnc9141-b could decrease MyHC expression. In addition, the intergenic region between lnc9141 and IRX5 was 2.3 kb, with a head-to-head orientation. The study also revealed the core regions of the lnc9141 and IRX5 promoter. Our study demonstrated that both lnc9141-a and -b expression inhibited bovine myoblast proliferation. However, lnc9141-b regulated Bax and MyHC expression. The regulatory mechanism of lnc9141-a and lnc9141-b needs to be further explored.

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Section: Discussionmentioning

confidence: 95%

lnc9141-a and -b Play a Different Role in Bovine Myoblast Proliferation, Apoptosis, and Differentiation

Zhang

Wang

et al. 2019

Molecular Therapy - Nucleic Acids

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“…In agreement with previous studies, ZP1 was highly expressed in the female chicken liver, which was indicated to be synthesized in the liver and transported via the bloodstream to the follicle (Bausek et al, 2000, 2004). CCDC152 , located on the W-chromosome, was highly expressed in the male kidney and liver, which was identified to reduce cell proliferation and migration through the JAK2/STAT signaling pathway in chicken (Lin et al, 2017). a PMM2 also had higher expression in male chickens, which was found in metabolic pathways such as amino sugar and nucleotide sugar metabolism and fructose and mannose metabolism (Kanehisa and Goto, 2000).…”

Section: Resultsmentioning

confidence: 99%

A Gene Expression Atlas of Lohmann White Chickens

Zhang

Wang

et al. 2022

Preprint

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Chicken (Gallus gallus domesticus) as one of the most economically important farm animals plays a major role in human food production and has been widely used as a key animal model that is presumed to be typical of avian and generally applicable to mammals in studies of developmental biology, virology, oncogenesis, and immunology. To get a better understanding of avian biology, global analysis of gene expression across multiple tissues is needed, which will aid genome annotation and support functional annotation of avian genes. We present a large-scale RNA-Seq dataset representing all the major organ systems from adult Lohmann White domesticus chickens. An open-access chicken tissue gene expression atlas (TGEA) (chickenatlas.avianscu.com) is presented based on the expression of 224 samples across 38 well-defined chicken tissues. Network-based cluster analysis of this dataset grouped genes according to dimensionality reduction and whole-body co-expression patterns, which were used to infer the function of uncharacterized genes from their co-expression with genes of known function. We describe the distribution and tissue specificity of 21,430 genes present in the chicken gene expression atlas and assign those signatures, where possible, to specific tissue populations or pathways. To better understand the functions of GPCRs in avian, we quantified the transcript levels of 254 nonodorant GPCRs in all tissues. Cluster analysis placed many GPCRs into expected anatomical and functional groups and predicted previously unidentified roles for less-studied receptors. We also produced this atlas to analyze male and female mRNA expression profiles in chicken somatic and gonad tissues. Our analyses uncovered numerous cases of somatic sex-biased mRNA expression, with the largest proportion found in the chicken pineal body, pituitary, and liver. This high-resolution gene expression atlas for chickens is, to our knowledge, the largest transcriptomic dataset of any avian to date. It provides a resource to improve the annotation of the current reference genome for chicken, presenting a model transcriptome for avian, and can be used as a resource for predicting roles for incompletely characterized GPCRs, exploring sex-biased specific gene expression, and for other purposes.

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“…GHR-AS-EST expression was the highest in the heart, followed by the liver, glandular stomach, and leg muscle, and it was the lowest in gizzard tissue ( Figure 1C). GHR-AS-EST suppresses GHR mRNA expression, but promotes the expression of both GHBP and 0.7-kb GHR The V1 GHR sense promoter reportedly drives the transcription of GHR sense transcript [27]. In this study, we identified an antisense promoter (ASP) of GHR located in intron 5 and 99 bp away from the 5′ end of GHR-AS-EST, which drives the expression of GHR-AS-EST (Figures 2A and S4).…”

Section: Ghr-as-est Is Strongly Expressed In Chicken Liver and Musclementioning

confidence: 73%

“…For qualitative analyses of the GHR V1 and ASP promoters, GFP luciferase reporters pV1-EGFP and pASP-EGFP were constructed by cloning the corresponding V1 and ASP regions into the pEGFP-N1 vector to substitute its CMV region, by PCR. The negative control vector pLinker-EGFP (without CMV region of pEGFP-N1) was constructed using deletion Ω-PCR as reported elsewhere [27]. To construct the Flag-tag expression plasmids, GH-CDS, GHR-FL-CDS, GHBP, 0.7-kb GHR and GHR-AS-EST were amplified by PCR from chicken liver cDNA.…”

Section: Construction Of Expression Plasmidsmentioning

confidence: 99%

Identiﬁcation of a novel antisense RNA that regulates growth hormone receptor expression in chickens

et al. 2019

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Natural antisense transcripts (NATs) are widely present in mammalian genomes and act as pivotal regulator molecules of gene expression. However, studies on NATs in the chicken are relatively rare. We identiﬁed a novel antisense transcript in the chicken, designated GHR-AS-EST , transcribed from the growth hormone receptor ( GHR ) locus, which encodes a well-known regulatory molecule of muscle development and fat deposition. GHR-AS-EST is predominantly expressed in the chicken liver and muscle tissues. GHR-AS-EST sequence conservation among vertebrates is weak. GHR-AS-EST forms an RNA–RNA duplex with GHBP to increase its stability, and regulates the expression of GHR sense transcripts at both the mRNA and protein levels. Further, GHR-AS-EST promotes cell proliferation by stimulating the expression of signaling factors in the JAK2/STAT pathway, and contributes to fat deposition via downregulating the expression of signaling factors in the JAK2/SOCS pathway in LMH hepatocellular carcinoma cells. We expect that the discovery of a NAT for a regulatory gene associated with cell proliferation and lipolysis will further our understanding of the molecular regulation of both muscle development and fat deposition.

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ChickenCCDC152shares an NFYB-regulated bidirectional promoter with agrowth hormone receptorantisense transcript and inhibits cells proliferation and migration

Cited by 5 publications

References 49 publications

lnc9141-a and -b Play a Different Role in Bovine Myoblast Proliferation, Apoptosis, and Differentiation

lnc9141-a and -b Play a Different Role in Bovine Myoblast Proliferation, Apoptosis, and Differentiation

A Gene Expression Atlas of Lohmann White Chickens

Identiﬁcation of a novel antisense RNA that regulates growth hormone receptor expression in chickens

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