Chicken Embryo Lethal Orphan Virus: Virus Localization and Histopathology in Chicken Embryos

Isolation of a Type 1 Avian Adenovirus (CELO) Mutant Which Was Nonpathogenic for the Chicken Embryo

“…The characteristics of the small-plaque virus in tissue culture and in embryonated eggs were essentially the same as those of the original virus (4,7,10). The virus that produced the large plaques was identified as a mutant since it differed markedly from the parent stock in plaque size and embryo pathogenicity.…”

“…When the diluted virus stocks were inoculated into the chorioallantoic sac of 11-day-old embryos, only the small-plaque virus produced dwarfing and mortality. Histological sections of the chorioallantoic membranes revealed intranuclear ininclusions in the endodermal cells (7). The median egg infectious dose of the small-plaque virus suspension was above 109/ml.…”

Replication of celo virus in the chicken chorio‐allantoic membrane

“…The main reason for the rapid accumulation of knowledge concerning the CELO virus appears to be its latency in embryonating chicken eggs, a concomitant relation to vaccine production (Yates and Fry, 1957;Mancini et al, 1969;Oxford and Potter, 1969), and oncogenicity for the hamster (Mancini et al, 1969;Sarma et al, 1965). Sarma et al (1965) first demonstrated that CELO virus inoculated by the subcutaneous route induced neoplasms in newborn Syrian hamsters.…”