Chicken Collagen Hydrolysate-derived Peptides Inhibit Tumor Necrosis Factor-α-induced Inflammatory Response in Endothelial Cells

Kouguchi, Tomomi; Ito, Ai; Iwai, Koji; Shimizu, Masaharu; Takahata, Yoshihisa; Suzuki, Takuya; Morimatsu, Fumiki; Tanabe, Soichi

doi:10.3136/fstr.18.667

Cited by 14 publications

(9 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hydrolysates of collagen and gelatin have also been reported to have potential anti‐inflammatory activity (Hartog, Cozijnsen, DE Vrij, & Garssen, ; Kouguchi et al, ; Ramadass et al, ; Sae‐leaw et al, ). Indeed, Sae‐leaw et al () reported that the Alcalase hydrolysis of seabass skin gelatin produced hydrolysates with anti‐inflammatory activity in RAW264.7 cells, decreasing levels of the pro‐inflammatory cytokines interleukin (IL)‐6 and IL‐1β.…”

Section: Introductionmentioning

confidence: 99%

“…A commercial collagen hydrolysate (Colnatur) has been reported to inhibit zymosan‐induced inflammation in mice and decreased the production of IL‐6 in whole blood cells (Hartog et al, ). Collagen, from chicken feet hydrolyzed using a fungal protease, produced peptides that inhibited tumor necrosis factor (TNF)‐α induced inflammation in human endothelial cells in culture (Kouguchi et al, ). In addition, a hydrolysate generated from the tryptic digestion of rat‐tail tendon collagen was reported to have anti‐inflammatory activity in vivo, reducing rectal bleeding and downregulating IL‐6, IL‐1β, and TNF‐α expression in colon tissues in dextran sodium sulfate‐induced colitis in mice (Ramadass et al, ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bioactivity of bovine lung hydrolysates prepared using papain, pepsin, and Alcalase

O’Sullivan

Lafarga

Hayes³

et al. 2017

J Food Biochem

View full text Add to dashboard Cite

Bovine lung tissue was hydrolyzed using three different proteases, papain, pepsin, or Alcalase, to generate hydrolysates. Hydrolysates showed little antioxidant activity in noncellular or cellular assays. The anti‐inflammatory activity of hydrolysates was assessed in RAW264.7 macrophages and Jurkat T cells. Treatment with the Alcalase hydrolysate significantly decreased the production of the pro‐inflammatory cytokines interleukin (IL)‐6 and IL‐1β in a dose dependent manner in RAW264.7 cells. Nitric oxide production also significantly decreased following treatment with this hydrolysate. The papain hydrolysate decreased IL‐6, IL‐1β, and NO production in RAW264.7 cells and IL‐2 production in Jurkat T cells. However, the decrease was likely due to cytotoxicity of this hydrolysate toward these cell lines. The pepsin hydrolysate had no effect on cytokine production in RAW264.7 cells and only slightly decreased IL‐2 production in Jurkat T cells. Practical applications Meat production generates large amounts of protein rich co‐products, which are often discarded as waste or sold as low value animal feeds. The enzymatic hydrolysis of these protein co‐products may produce hydrolysates that are capable of reducing oxidation or inflammation. Novel hydrolysates were generated from the hydrolysis of bovine lung and results from this study indicate that the hydrolysis of bovine lung using the commercially available protease Alcalase may have potential as an anti‐inflammatory agent.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioactivity of bovine lung hydrolysates prepared using papain, pepsin, and Alcalase

O’Sullivan

Lafarga

Hayes³

et al. 2017

J Food Biochem

View full text Add to dashboard Cite

show abstract

“…12) Pro-Hyp has been reported to promote synthesis of hyaluronic acid 13) and formation of articular cartilage, 14) and Pro-Hyp and Hyp-Gly inhibit tumor necrosis factor induced inflammatory response. 15) Gly-Pro reduces the blood pressure. 16) Various beneficial effects observed after ingestion of collagen hydrolysate are considered to be closely related to these metabolized peptides.…”

mentioning

confidence: 99%

Absorption and Urinary Excretion of Peptides after Collagen Tripeptide Ingestion in Humans

Yamamoto

Deguchi

Onuma

et al. 2016

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Collagen tripeptide (CTP) is a collagen hydrolysate containing a high concentration of tripeptides with aGly-X-Y sequence, such as Gly-Pro-Hyp. To test the effects of this preparation, we compared the absorption of peptides in humans after ingestion of a tripeptide fraction of CTP (CTP-100), a CTP preparation containing ca. 50% Gly-X-Y tripeptides (CTP-50), and a collagen peptide that did not contain tripeptides (CP). The postprandial levels of Gly-Pro-Hyp and Pro-Hyp in the plasma increased in those subjects who ingested CTP-100 and CTP-50, and were higher with greater Gly-Pro-Hyp ingestion. This demonstrated that collagen hydrolysates were efficiently absorbed when the collagen was ingested in the tripeptide form. Gly-Pro-Hyp and Pro-Hyp were also found in the urine after ingestion of CTP-100 or CTP-50. Similar to the results for the plasma concentration, the urinary excretion of Gly-Pro-Hyp and Pro-Hyp was also dependent on the amount of Gly-Pro-Hyp ingested. This indicates that ingested Gly-Pro-Hyp and generated Pro-Hyp were relatively stable in the body and were transported to the urine in the peptide form. The concentration of HypGly in the plasma was low after the ingestion of CP and CTP-100 but higher after the ingestion of CTP-50. Overall, our results suggest that tripeptides derived from collagen are absorbed efficiently by the body.

show abstract

“…2) In our previous study, these peptides reduced the expression of such inflammation markers as ICAM-1, VCAM-1, IL-8, and inducible nitric oxide synthase (iNOS) which were up-regulated by TNF-. 24) These results suggest that the modulatory effects of CCH on the circulation system, for example the improvement of arterial flexibility, might be due to the inhibition of endothelial inflammation.…”

Section: Discussionmentioning

confidence: 95%

Effects of a Chicken Collagen Hydrolysate on the Circulation System in Subjects with Mild Hypertension or High-Normal Blood Pressure

Kouguchi

Ohmori

Shimizu

et al. 2013

Bioscience, Biotechnology, and Biochemistry

Self Cite

View full text Add to dashboard Cite

We investigated the effects of a chicken collagen hydrolysate (CCH) on the circulation system in humans. A total of 58 subjects with either mild hypertension (systolic blood pressure (SBP) of 140-159 mmHg or diastolic blood pressure (DBP) 90-99 mmHg) or high-normal blood pressure (SBP 130-139 mmHg or DBP 85-89 mmHg) were assigned to two groups, one involving a placebo and the other, the test food (including CCH of 2.9 g/d). The parameters related to each subject's circulation system were monitored over the study period of 18 weeks. The Δbrachial-ankle pulse wave velocity (baPWV), an indicator of arterial stiffness and marker of vascular damage, was significantly lower in the test food group than in the placebo group during the treatment period. The blood pressure in the test food group was also significantly lower than that in the placebo group, while the serum nitrogen oxide was higher in the test food group after the treatment. These results suggest that CCH exerted modulatory effects on the human circulation system.

show abstract

Chicken Collagen Hydrolysate-derived Peptides Inhibit Tumor Necrosis Factor-α-induced Inflammatory Response in Endothelial Cells

Cited by 14 publications

References 16 publications

Bioactivity of bovine lung hydrolysates prepared using papain, pepsin, and Alcalase

Bioactivity of bovine lung hydrolysates prepared using papain, pepsin, and Alcalase

Absorption and Urinary Excretion of Peptides after Collagen Tripeptide Ingestion in Humans

Effects of a Chicken Collagen Hydrolysate on the Circulation System in Subjects with Mild Hypertension or High-Normal Blood Pressure

Contact Info

Product

Resources

About