Chicken B-cell marker chB6 (Bu-1) is a highly glycosylated protein of unique structure

Abstract

The chB6 molecule is expressed on chicken B cells throughout most of their development, as well as on some non-lymphoid cells. It has long been used as an allotypic marker in important studies of B-cell development, though its function is unknown. We isolated a chB6 cDNA by expression cloning and sequenced two further alleles following polymerase chain reaction amplification. The results show that chB6 is a typical type I transmembrane protein, highly glycosylated in the extracellular region and carrying a large intracellular region. It has no recognizable similarity to known mammalian molecules and thus represents a unique B-cell marker. Its presence in chickens may be related to differences in the properties of B-cell development between chickens and mammalian species. The sequences of the different alleles of this gene revealed a higher level of polymorphism than expected. A restriction fragment length polymorphism linked to the CHB6 gene has been used to determine its location on the linkage map of the chicken genome, which will allow the definitive evaluation of reported associations with disease resistance.

Effects of age and gonadal steroids on the localization of antigen-presenting cells, and T and B cells in the chicken oviduct

“…The monoclonal antibody to Bu-1 used in this study recognizes immature cells but not the antibody-producing plasma cells that are mature cells (Veromaa et al, 1988b;Mansikka et al, 1989;Tregaskes et al, 1996) (Yoshimura et al, 1997a Wira, 1979). Severe atrophy of the bursa and an increase in plasma cell population in the oviduct have been observed in DEStreated chickens (Khan et al, 1997).…”

Clearance of EntericSalmonella entericaSerovar Typhimurium in Chickens Is Independent of B-Cell Function

“…2A). Phycoerythrinlabeled anti-BU-1 (Cambridge Bioscience, Cambridge, United Kingdom) recognizes chicken B cells (23,25) and was used for fluorescence-activated cell sorting analysis. As expected, both surgical bursectomy and cylophosphamide treatment effectively removed B cells from the spleen (0.34% and 0.63% Bu1 ϩ cells, respectively, compared with 19.80% with intact animals) (Fig.…”

A B-cell targeting virus disrupts potentially protective genomic methylation patterns in lymphoid tissue by increasing global 5-hydroxmethylcytosine levels

“…This observed 5hmC reduction in bursal tissue sections was validated by a dot blot assay ( Figure 1B) which shows a progressive reduction in genomic 5hmC from ED16 to seven days posthatch (D7). To confirm that the proliferating follicles did contain B-cells we co-stained ED18 bursal sections with Bu-1, an avian B-cell marker [26], and either an antibody directed to 5mC or 5hmC. We found that Bu-1 only stained cells within each follicle and the majority of these B-cells were also highly enriched for 5mC (Figure 2A).…”