Chick production by in vitro fertilization of the fowl ovum

Tanaka, Ken Ichiro; Wada, Tetsuya; Katoh, Osamu; Nishio, Yasunori; Hertelendy, F.

doi:10.1530/jrf.0.1000447

Cited by 17 publications

(13 citation statements)

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“…In addition, the fimbriae of oviduct should be closed by suture line after the ova located at the anterior magnum, so as to avoid retrograde expulsion into the abdominal cavity. This study also showed that the fertilization rate of IVF was 18% after oocytes ovulated spontaneously, compared with the previous rates of 58% (Tanaka et al. 1994) and 15% (Olszan′ska et al.…”

Section: Discussionsupporting

confidence: 47%

“…The modified Ringer’s solution consisted of 145 m m NaCl, 2.68 m m KCl, 2.68 m m CaCl 2 , 0.24 m m NaHCO 3 , 1 g/l streptomycin sulphate and 5000 IU/l penicillin (Tanaka et al. 1994).…”

Section: Methodsmentioning

confidence: 99%

“…1991). Tanaka produced viable chicken by IVF and transferred the fertilized ova into the oviduct of recipient hens (Tanaka et al. 1994).…”

Section: Introductionmentioning

confidence: 99%

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The Influencing Factor of In Vitro Fertilization and Embryonic Transfer in the Domestic Fowl (Gallus domesticus)

Chen

et al. 2012

Reprod Domestic Animals

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ContentsThis study was conducted to explore the influencing factors of ova in vitro fertilization (IVF) and transfer of the fertilized ova into the oviduct of recipient hens. The efficiency of fertilization was compared using three aspects: (i) the different time of ova collection and transfer, (ii) egg-laying period of recipient hen; and (iii) semen volume. The following results are observed: 72%, 40% and 0% of ova were found in ovarian sac in 3040 min, 5060 min and more than 90 min post-oviposition, respectively; 20%, 18%, 14% and 5.8% of ova were fertilized with 0.1, 0.2, 0.5 and 1.0 ml semen, respectively; and 33% and 100% of healthy chickens were hatched from fertile ova with 0.1 and 0.5 ml of semen, respectively. All oocytes obtained from ovary and mid-oviduct were unfertilized. Embryos were transferred into recipient hens 30 min ± 10min post-oviposition, and 70% of shelled eggs were produced. There were no eggs produced in the other transfer times. This demonstrated that live chicken can be obtained by IVF of ova collected shortly after oviposition. It was important that the ovum was transferred into the oviduct infundibulum of recipient hens immediately or shortly after oviposition.

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Section: Discussionsupporting

confidence: 47%

“…The modified Ringer’s solution consisted of 145 m m NaCl, 2.68 m m KCl, 2.68 m m CaCl 2 , 0.24 m m NaHCO 3 , 1 g/l streptomycin sulphate and 5000 IU/l penicillin (Tanaka et al. 1994).…”

Section: Methodsmentioning

confidence: 99%

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The Influencing Factor of In Vitro Fertilization and Embryonic Transfer in the Domestic Fowl (Gallus domesticus)

Chen

et al. 2012

Reprod Domestic Animals

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“…However, it is possible to fertilize eggs through AI with frozen samples. The difficulty of using techniques such as intramagnal fertilization [5] and IVF [21,33] urges for the development of cryopreservation techniques allowing a more efficient sperm conservation.…”

Section: Individual Cryopreservation Of Three Avian Speciesmentioning

confidence: 99%

Individual Cryopreservation With Dimethyl Sulfoxide and Polyvinylpyrrolidone of Ejaculates and Pooled Semen of Three Avian Species

Herrera

Quintana

López

et al. 2005

Archives of Andrology

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Artificial insemination (AI) has been used for avian reproduction due to the discovery of cryoprotectants extending its usefulness both in production of domestic fowl and conservation of wild species. The goal of this study was to assess the effect on domestic and wild fowl pooled semen and individual ejaculate cryopreservation with dimethyl sulfoxide (DMSO) and polyvinylpyrrolidone (PVP). Twenty ejaculates and twenty samples of pooled semen of roosters, pheasants and hawks were frozen in media containing DMSO or PVP. DMSO and PVP cryopreservation are equally effective both for ejaculates and pooled semen. Even PVP is a good alternative since no significant difference was found when compared to DMSO. The fertilizing capacity of fresh and cryopreserved pooled semen was analyzed through AI of hens and female pheasants. Similar fertility rates using DMSO, PVP or frozen-thawed samples demonstrated that reproduction is possible through the use of cryopreserved semen. In the case of female pheasants, the same values were obtained with both cryopreserved and fresh semen.

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“…Furthermore, Nakanishi et al [11] demonstrated the fertility competency of multiple-ovulated eggs in the chicken after IVF. Tanaka et al [12] produced viable chicks by implanting in vitro-fertilized ova into the oviduct of recipient hens followed by incubation of the eggs. Recently, Olszańska et al [13] investigated development of quail embryos after IVF using in vitroovulated oocytes with a special emphasis on the link between the steps of ovulation and fertilization and with the early cleavage stages under in vitro conditions.…”

Section: Introductionmentioning

confidence: 99%

Fertilization and Development of Quail Oocytes after Intracytoplasmic Sperm Injection1

Hrabia

Takagi

Ono³

et al. 2003

Biology of Reproduction

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The present study was conducted to establish the intracytoplasmic sperm injection (ICSI) method for in vitro fertilization and development in quail. The efficiency of fertilization of oocytes was compared 1) between spontaneous and premature ovulation and 2) among testicular round spermatids, elongated spermatids, and immature and mature spermatozoa. The oocytes were injected with a single spermatozoon or spermatid and cultured for 24 h. Cell division was histologically observed with hematoxylin-eosin (HE) and a nucleus-specific fluorescent dye (DAPI). Five of 30 (16.6%) and 4 of 30 (13.3%) oocytes injected with mature sperm were fertilized in the spontaneous and induced ovulation group, respectively. Those embryos showed development at stages II-VII. Half the number (three of six) of the oocytes injected with testicular spermatozoa were fertilized and developed to stages IV-VII, and two of five oocytes injected with elongated spermatids were fertilized and developed to stage VI. All ooocytes injected with round spermatids were unfertilized. The results demonstrate that intracytoplasmic injection of a single sperm into quail oocyte can activate the oocyte and lead to fertilization. Oocytes prematurely ovulated are capable of fertilizing with mature sperm as are those spontaneously ovulated. In addition, the results suggest that the testicular round spermatids may not possess sufficient oocyte-activating potency but that the elongated spermatids and immature spermatozoa are competent to participate in fertilization and early embryonic development in quail.

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Chick production by in vitro fertilization of the fowl ovum

Abstract: The aim of this study was

Cited by 17 publications

References 0 publications

The Influencing Factor of In Vitro Fertilization and Embryonic Transfer in the Domestic Fowl (Gallus domesticus)

The Influencing Factor of In Vitro Fertilization and Embryonic Transfer in the Domestic Fowl (Gallus domesticus)

Individual Cryopreservation With Dimethyl Sulfoxide and Polyvinylpyrrolidone of Ejaculates and Pooled Semen of Three Avian Species

Fertilization and Development of Quail Oocytes after Intracytoplasmic Sperm Injection1

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