Chick embryo lethal orphan virus can be polymer-coated and retargeted to infect mammalian cells

Preclinical Efficacy and Safety Profile of Allometrically Scaled Doses of Doxycycline Used to Turn “On” Therapeutic Transgene Expression from High-Capacity Adenoviral Vectors in a Glioma Model

“…Neutralizing antibodies have not been detected in the circulation of GBM-bearing animals treated with HC-Ad-TetOn-Flt3L + HCAd-TK delivered into the brain tumor mass. Although opsonizing factors could direct the HC-Ad vector to phagocytes, 46 diminishing therapeutic transgene expression, previous work from our group has not encountered this possibility after delivery of the vectors into either the normal brain parenchyma, or into a tumor mass growing within the brain, even in the presence of a systemic immunization against the adenovirus vectors. 5,11,[30][31][32] In addition, hematological and biochemical parameters were all normal in both DOX-treated animals and controls (Supplementary Table S1).…”

Identification of HI-Like Loop in CELO Adenovirus Fiber for Incorporation of Receptor Binding Motifs

“…Use of the avian Ad CELO bypasses the immunity limitation but is still dependent upon CAR expression. It was recently shown that both drawbacks can simultaneously be partially overcome by using an alternative avian Ad CELO vector system modified by polymer coating to retarget the virus to the fibroblast growth factor receptor (34). However, CELO-mediated gene transfer was limited in this case by fibroblast growth factor receptor expres- sion.…”

“…To determine the adenoviral copy number, real-time PCR was performed as described previously (34). Briefly, total DNA from cells transduced with Ad5-SEAP, CELO-SEAP, or CELOSEAP-HIRGD was extracted with a DNA purification kit (Promega) according to the manufacturer's instructions.…”

“…Recent studies have shown that CELO virus can be used as a gene transfer vehicle for gene therapy (24,34) and gene vaccination (10). However, the usefulness of CELO-based vectors is significantly limited by their poor transduction efficiency of CAR-deficient cells compared to CAR-positive cells (24,35).…”

“…The chicken embryo lethal orphan (CELO) virus is representative of type 1 fowl adenoviruses (Ads) (3,24,25,34). Similar to mammalian Ads, the CELO virus has an icosahedral capsid comprised of hexons and pentons as major subunits (35).…”