Chick embryo chorioallantoic membrane (CAM): an alternative predictive model in acute toxicological studies for anti-cancer drugs

Kue, Chin Siang; Tan, Kae Yi; Lam, May Lynn; Lee, Hong Boon

doi:10.1538/expanim.14-0059

Cited by 131 publications

(101 citation statements)

References 43 publications

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“…The CAM model is a preclinical model widely used for vascular and antivascular effects of therapeutic agents in vivo [19]. It is one of the most well-characterized and useful models.…”

Section: Discussionmentioning

confidence: 99%

Transcranial Yellow, Red, and Infrared Laser and LED Stimulation: Changes of Vascular Parameters in a Chick Embryo Model

Litscher¹,

Min²,

Passegger³

et al. 2015

Integr Med Int

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Background: The use of transcranial laser or light-emitting diode (LED) therapy in the treatment of different neurological diseases is attracting increasing attention. The main goal of this study was to investigate different kinds of scientifically and commercially available laser (yellow, red, and infrared) and LED stimulation systems for the first time using a chick embryo model under a fixed human cadaver (scalp and/or skull) and without this anatomical preparation. Methods: In the present study, the extra-embryonic vasculature of a 1-week-old chick embryo chorioallantoic membrane (CAM) was investigated. For stimulation, four different systems were used (infrared LED and yellow, red, and infrared laser). Four measurement periods were compared: before stimulation, immediately after stimulation onset, immediately after the 10-min stimulation was turned off, and 6 min after stimulation had ended. Special image processing programs developed at the Beijing University of Science and Technology were used for data analysis. Results: The different kinds of laser induced different effects, e.g. the direct stimulation with yellow laser led to initial vasoconstriction (-17%), whereas yellow laser stimulation through a human skull resulted in an increase in blood volume. These effects could only be observed after mathematical processing of the images. Conclusion: After intensive basic research (e.g. CAM model), transcranial laser stimulation may open up new therapies for lifestyle-related diseases such as stroke, dementia, Alzheimer's or possibly Parkinson's disease.

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“…The CAM model is a preclinical model widely used for vascular and antivascular effects of therapeutic agents in vivo [19]. It is one of the most well-characterized and useful models.…”

Section: Discussionmentioning

confidence: 99%

Transcranial Yellow, Red, and Infrared Laser and LED Stimulation: Changes of Vascular Parameters in a Chick Embryo Model

Litscher¹,

Min²,

Passegger³

et al. 2015

Integr Med Int

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“…They prey in other Gram-negative bacteria giving them potential as biological control agents and so its LD 50 was determined in the host using the method of Saganuwan (2011). In this regard quick chick embryo chorioallantoic membrane (CAM) assay was used as an alternative predictive model in acute toxicological studies for cyclophosphamide, cisplatin, vincristine, carmustine, campothecin, aloin, mitomycin C, actinomycin D, melphalan and paclitaxel (Kue et al, 2015) using the method of Saganuwan (2011). Any drug has the potential to cause an adverse reaction which is unwanted, unpleasant, noxious or harmful consequence usually associated with the use of a medication that has been administered at a standard dose by the proper route, for the purpose of prophylaxis, diagnosis or treatment (Wen et al, 2014).…”

Section: Advantages Of Toxicity Studiesmentioning

confidence: 99%

Toxicity studies of drugs and chemicals in animals: an overview

Saganuwan¹

2017

BJVM

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SummarySaganuwan, S. A., 2017. Toxicity studies of drugs and chemicals in animals: An overview. Bulg. J. Vet. Med., 20, No 4, Toxicity study is the investigation of either short or long-term toxic effects of a drug or chemical on animals. The toxicity is dose-dependent as asserted by Paracelsus over 500 years ago. However, short-term toxic effect is determined using median lethal dose (LD 50 ) first introduced by Trevan in 1927 and revised many times. Presently there is a growing preponderance of rejection of scientific papers on acute toxicity study, simply because of the belief that in the current hazard and safety assessment of drugs and chemicals, LD 50 values are no longer used. In view of this, literature search was carried out with a view to investigating the relevance of LD 50 in development and assessment of drugs and chemicals. The findings revealed that in the past, many animals had been used for LD 50 determination. OECD has reduced the number of test animals to 5-15 and presently it is further reduced to 2-6. Acute toxicity study is being carried out in medicinal plants research and in the study of patent medicine. Although the application of LD 50 has been drastically reduced, it is still applied and accepted in some parts of the world. Moreover, animals on which LD 50 tests are conducted, should be allowed to die to see the end effect of the test drug or chemical because euthanisia of test animals may mask some toxicity signs of the test agents. Therefore, toxicity study of drugs and chemicals is a scientific process necessary for discovery and development of drugs as well as identification of potential toxicants.

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“…The experiments were performed according to published procedures and standard animal treatment practices [42]. Escalating doses of compounds dissolved in milliQ water and pH adjusted to 7.4 were administrated to chick embryos on EDD13 via intravenous injection.…”

Section: Chick Embryo Acute Toxicitymentioning

confidence: 99%

Tunable phosphatase-sensitive stable prodrugs of 5-aminolevulinic acid for tumor fluorescence photodetection

Babič

Herceg

Ateb

et al. 2016

Journal of Controlled Release

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5-aminolevulinic acid (5-ALA) has been at the forefront of small molecule based fluorescence-guided tumor resection and photodynamic therapy. 5-ALA and two of its esters received marketing authorization but suffer from several major limitations, namely low stability and poor pharmacokinetic profile. Here, we present a new class of 5-ALA derivatives aiming at the stabilization of 5-ALA by incorporating a phosphatase sensitive group, with or without self-cleavable linker. Compared to 5-ALA hexyl ester (5-ALA-Hex), these compounds display an excellent stability under acidic, basic and physiological conditions. The activation and conversion into the 5-ALA is controlled and can be structure-tailored. The prodrugs display reduced acute toxicity compared to 5-ALA-Hex with superior dose response profiles of protoporphyrin IX synthesis and fluorescence intensity in human glioblastoma cells in vitro. Clinically relevant fluorescence kinetics in vivo shown in U87Mg glioblastoma spheroid tumor model in chick embryos provide a solid basis for their further development and translation to clinical fluorescence guided tumor resection and photodynamic therapy.

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Chick embryo chorioallantoic membrane (CAM): an alternative predictive model in acute toxicological studies for anti-cancer drugs

Cited by 131 publications

References 43 publications

Transcranial Yellow, Red, and Infrared Laser and LED Stimulation: Changes of Vascular Parameters in a Chick Embryo Model

Transcranial Yellow, Red, and Infrared Laser and LED Stimulation: Changes of Vascular Parameters in a Chick Embryo Model

Toxicity studies of drugs and chemicals in animals: an overview

Tunable phosphatase-sensitive stable prodrugs of 5-aminolevulinic acid for tumor fluorescence photodetection

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