Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer

Abstract

New therapy development is critically needed for ovarian cancer. We used the chicken egg CAM assay to evaluate efficacy of anticancer drug delivery using recently developed biodegradable PMO (periodic mesoporous organosilica) nanoparticles. Human ovarian cancer cells were transplanted onto the CAM membrane of fertilized eggs, resulting in rapid tumor formation. The tumor closely resembles cancer patient tumor and contains extracellular matrix as well as stromal cells and extensive vasculature. PMO nanoparticles loaded with doxorubicin were injected intravenously into the chicken egg resulting in elimination of the tumor. No significant damage to various organs in the chicken embryo occurred. In contrast, injection of free doxorubicin caused widespread organ damage, even when less amount was administered. The lack of toxic effect of nanoparticle loaded doxorubicin was associated with specific delivery of doxorubicin to the tumor. Furthermore, we observed excellent tumor accumulation of the nanoparticles. Lastly, a tumor could be established in the egg using tumor samples from ovarian cancer patients and that our nanoparticles were effective in eliminating the tumor. These results point to the remarkable efficacy of our nanoparticle based drug delivery system and suggests the value of the chicken egg tumor model for testing novel therapies for ovarian cancer.

Biodegradable Periodic Mesoporous Organosilica (BPMO) Loaded with Daunorubicin: A Promising Nanoparticle‐Based Anticancer Drug

“…The tumor accumulation was observed three days after the injection. We believe that BPMO‘s prolonged blood circulation contributes to tumor accumulation through the EPR (enhanced permeability and retention) effect …”

“…Synthesis of biodegradable PMO : Synthesis of BPMO was slightly modified from a reported procedure . In particular, RBITC was attached into the framework by co‐synthesis method: 2.5 mg of RBITC was dissolved in ethanol (EtOH, 5 mL) and then 3‐aminotriethoxysilane (APTES, 6 L, 2.6×10 −2 mmol) was added.…”

Monitoring of Tumor Growth and Vascularization with Repetitive Ultrasonography in the Chicken Chorioallantoic-Membrane-Assay.

“…In many published articles however, tumor growth is only measured twodimensionally or estimated using surrogate parameters like bioluminescence (31). Furthermore, end-point-analysis in the form of size measuring (45), cell count in ow cytometry of digested tumors, or weight measurements (46) have been utilized or the determination of tumor size.…”

Enthusiastic discussions on solid physic and material science at SPMS2019

“…Prof. Thang's center also synthesized a lot of MOF with the named VNU-number such as VNU-1, VNU-2, etc. and their application in energy, environment, catalysis, health care [10][11][12][13][14][15][16][17] . Dr. Phong D. Tran (University of Science and Technology of Hanoi, Vietnam) described the current state-of-the-art of the solar water splitting research.…”