Chestnut shells (Italian cultivar “Marrone di Roccadaspide” PGI): Antioxidant activity and chemical investigation with in depth LC-HRMS/MSn rationalization of tannins

Cerulli, Antonietta; Napolitano, Assunta; Masullo, Milena; Hošek, Jan; Pìzza, Cosimo; Piacente, Sonia

doi:10.1016/j.foodres.2019.108787

Cited by 38 publications

(50 citation statements)

References 60 publications

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“…Finally, the intracellular fluorescence of the dichlorofluorescein product was measured by a Fluostar Omega MicroplateReader (BMG Labtech) using the λ (ex./em.) = 480/530 nm [11].…”

Section: Determination Of Intracellular Ros Scavenging Activitymentioning

confidence: 99%

“…After the pre-incubation, 2 μl of LPS (1 μg/mL) was added to each well to activate the NF-kB pathway. Measurements were taken about 24 h after treatment, and the activity of NF-kB was determined spectrophotometrically by a Fluostar Omega Microplate Reader (BMG Labtech) [11].…”

Section: Determination Of Intracellular Nf-κb Activitymentioning

confidence: 99%

“…Our previous investigation on "Marrone di Roccadaspide" shell extract, rich in hydrolyzable and condensed tannins as well as flavonoids and triterpenoids, showed its capability to modulate the pro-inflammatory transcriptional factor NF-κB after lipopolysaccharide (LPS) stimulation and to exert a good antioxidant activity by a cellbased in vitro test [11]. Moreover, phenolic compounds isolated from the MeOH extract of "Marrone di Roccadaspide" leaves were reported to prevent UV-induced/cell damage [12].…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant and In Vitro Preliminary Anti-Inflammatory Activity of Castanea sativa (Italian Cultivar “Marrone di Roccadaspide” PGI) Burs, Leaves, and Chestnuts Extracts and Their Metabolite Profiles by LC-ESI/LTQOrbitrap/MS/MS

et al. 2021

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The Italian “Marrone di Roccadaspide” (Castanea sativa), a labeled Protected Geographical Indication (PGI) product, represents an important economic resource for the Italian market. With the aim to give an interesting opportunity to use chestnuts by-products for the development of nutraceutical and/or cosmetic formulations, the investigation of burs and leaves along with chestnuts of C. sativa, cultivar “Marrone di Roccadaspide”, has been performed. The phenolic, tannin, and flavonoid content of the MeOH extracts of “Marrone di Roccadaspide” burs, leaves, and chestnuts as well as their antioxidant activity by spectrophotometric methods (1,1-diphenyl-2-picrylhydrazyl (DPPH), Trolox Equivalent Antioxidant Capacity (TEAC), and Ferric Reducing Antioxidant Power (FRAP) have been evaluated. Furthermore, a cell-based antioxidant in vitro test along with in vitro assays for the evaluation of the ability to reduce nuclear factor-kappa B (NF-κB) activation and nitric oxide (NO) production have been carried out. In order to identify the secondary metabolites responsible for the high phenolic content and the strong antioxidant activity shown by leaves and burs extracts, and to highlight the differences between their chemical composition, the analysis of the metabolite profile of the MeOH extracts obtained from both by-products and chestnuts by liquid chromatography coupled to electrospray ionization and multiple-stage linear ion-trap and Orbitrap high-resolution mass spectrometry (LC-(-)ESI/LTQOrbitrap/MS/MS) has been performed. LC-MS analysis allowed the identification of different classes of specialized metabolites including hydrolyzable tannins, flavonoids, ellagic acid and phenol glucoside derivatives, and triterpenoids as well as polar lipids. Our results show how the antioxidant activity of the extracts can be correlated to their high tannins and flavonoids content while polar lipids occurring in the MeOH extract of the leaves could contribute to determining its higher anti-inflammatory activity.

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“…Finally, the intracellular fluorescence of the dichlorofluorescein product was measured by a Fluostar Omega MicroplateReader (BMG Labtech) using the λ (ex./em.) = 480/530 nm [11].…”

Section: Determination Of Intracellular Ros Scavenging Activitymentioning

confidence: 99%

Section: Determination Of Intracellular Nf-κb Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antioxidant and In Vitro Preliminary Anti-Inflammatory Activity of Castanea sativa (Italian Cultivar “Marrone di Roccadaspide” PGI) Burs, Leaves, and Chestnuts Extracts and Their Metabolite Profiles by LC-ESI/LTQOrbitrap/MS/MS

et al. 2021

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“…Regarding chestnut by-products, Costa-Trigo and co-workers suggested the application of chestnut burs extract for the production of culture media suitable for the growth of a wide range of microorganisms [ 4 ]. Moreover, chestnut shells of an Italian cultivar “Marrone di Roccadaspide” PGI (Protected Geographical Indication) were found to be endowed with antioxidant activity linked to their high content of tannins [ 5 ]. The abundance of total phenols and hydrolysable tannins confers to chestnut grove by-products interesting anti-inflammatory activity, as demonstrated by the ability of Castanea shell extracts to reduce the levels of cytokines and other biomarkers of inflammation in several experimental models [ 6 , 7 , 8 , 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Leaves and Spiny Burs of Castanea Sativa from an Experimental Chestnut Grove: Metabolomic Analysis and Anti-Neuroinflammatory Activity

et al. 2020

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Castanea sativa cultivation has been present in Mediterranean regions since ancient times. In order to promote a circular economy, it is of great importance to valorize chestnut groves’ by-products. In this study, leaves and spiny burs from twenty-four Castanea trees were analyzed by 1H NMR metabolomics to provide an overview of their phytochemical profile. The Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) performed on these data allowed us to distinguish ‘Marrone’ from ‘Castagna’, since the latter were generally more enriched with secondary metabolites, in particular, flavonoids (astragalin, isorhamnetin glucoside, and myricitrin) were dominant. Knowing that microglia are involved in mediating the oxidative and inflammatory response of the central nervous system, the potential anti-inflammatory effects of extracts derived from leaves and spiny burs were evaluated in a neuroinflammatory cell model: BV-2 microglia cells. The tested extracts showed cytoprotective activity (at 0.1 and 0.5 mg/mL) after inflammation induction by 5 µg/mL lipopolysaccharide (LPS). In addition, the transcriptional levels of IL-1β, TNF-α, and NF-kB expression induced by LPS were significantly decreased by cell incubation with spiny burs and leaves extracts. Taken together, the obtained results are promising and represent an important step to encourage recycling and valorization of chestnut byproducts, usually considered “waste”.

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“…First, Al-Dobaib [ 31 ] reported that tannins can form complexes with feed proteins and reduce their utilization by rumen microorganisms, thereby improving the nitrogen utilization rate and reducing nitrogen emissions. PICB is mainly composed of HTs such as ellagic acid, with a small percentage of CTs such as proanthocyanins [ 14 , 32 ]. CTs can form complexes with feed proteins and decrease their degradability [ 33 ], thus reducing the NH 3 -N concentration.…”

Section: Discussionmentioning

confidence: 99%

Pilot Study of the Effects of Polyphenols from Chestnut Involucre on Methane Production, Volatile Fatty Acids, and Ammonia Concentration during In Vitro Rumen Fermentation

Wang

et al. 2021

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Nutritional strategies can be employed to mitigate greenhouse emissions from ruminants. This article investigates the effects of polyphenols extracted from the involucres of Castanea mollissima Blume (PICB) on in vitro rumen fermentation. Three healthy Angus bulls (350 ± 50 kg), with permanent rumen fistula, were used as the donors of rumen fluids. A basic diet was supplemented with five doses of PICB (0%–0.5% dry matter (DM)), replicated thrice for each dose. Volatile fatty acids (VFAs), ammonia nitrogen concentration (NH3-N), and methane (CH4) yield were measured after 24 h of in vitro fermentation, and gas production was monitored for 96 h. The trial was carried out over three runs. The results showed that the addition of PICB significantly reduced NH3-N (p < 0.05) compared to control. The 0.1%–0.4% PICB significantly decreased acetic acid content (p < 0.05). Addition of 0.2% and 0.3% PICB significantly increased the propionic acid content (p < 0.05) and reduced the acetic acid/propionic acid ratio, CH4 content, and yield (p < 0.05). A highly significant quadratic response was shown, with increasing PICB levels for all the parameters abovementioned (p < 0.01). The increases in PICB concentration resulted in a highly significant linear and quadratic response by 96-h dynamic fermentation parameters (p < 0.01). Our results indicate that 0.2% PICB had the best effect on in-vitro rumen fermentation efficiency and reduced greenhouse gas production.

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Chestnut shells (Italian cultivar “Marrone di Roccadaspide” PGI): Antioxidant activity and chemical investigation with in depth LC-HRMS/MSn rationalization of tannins

Cited by 38 publications

References 60 publications

Antioxidant and In Vitro Preliminary Anti-Inflammatory Activity of Castanea sativa (Italian Cultivar “Marrone di Roccadaspide” PGI) Burs, Leaves, and Chestnuts Extracts and Their Metabolite Profiles by LC-ESI/LTQOrbitrap/MS/MS

Antioxidant and In Vitro Preliminary Anti-Inflammatory Activity of Castanea sativa (Italian Cultivar “Marrone di Roccadaspide” PGI) Burs, Leaves, and Chestnuts Extracts and Their Metabolite Profiles by LC-ESI/LTQOrbitrap/MS/MS

Leaves and Spiny Burs of Castanea Sativa from an Experimental Chestnut Grove: Metabolomic Analysis and Anti-Neuroinflammatory Activity

Pilot Study of the Effects of Polyphenols from Chestnut Involucre on Methane Production, Volatile Fatty Acids, and Ammonia Concentration during In Vitro Rumen Fermentation

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