Chest wall repair with engineered fetal bone grafts: an efficacy analysis in an autologous leporine model

Klein, Jessica; Turner, Christopher G.; Ahmed, Azra; Steigman, Shaun A.; Zurakowski, David; Fauza, Dario O.

doi:10.1016/j.jpedsurg.2010.02.116

Cited by 33 publications

(30 citation statements)

References 25 publications

(30 reference statements)

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“…These two markers were not proposed by International Society for Cellular Therapy (ISCT; Dominici et al, 2006), however they are often used to detect MSC populations. There are many other studies of AF-MSCs, which found theses markers to be expressed in human (In't Anker et al, 2003;Tsai et al, 2004;De Coppi et al, 2007), rabbit (Steigman et al, 2009;Klein et al, 2010;DeKoninck et al, 2013;Fei et al, 2013;Kováč et al, 2016) and other species (Park et al, 2011;Chen et al, 2011;Nadri and Soleimani, 2007;Iacono et al, 2012;Choi et al, 2013). Investigators therefore use CD29 and CD44 together with some of molecular markers proposed for human MSCs by ISCT.…”

Section: Resultsmentioning

confidence: 99%

“…Although rAF-MSCs were previously defined by a few studies (Steigman et al, 2009;Klein et al, 2010;DeKoninck et al, 2013;Fei et al, 2013), some of the MSC markers, like CD73, CD105 and 166 were not previously screened in rAF-MSCs. A negative expression of surface markers detected by flow cytometry could also be possibly attributed to antibodies specificity.…”

Section: Resultsmentioning

confidence: 99%

“…To date, AF-MSCs have been isolated from various animal species for preclinical research (Steigman et al, 2009;Klein et al, 2010;Chen et al, 2011;Filioli Uranio et al, 2011;Choi et al, 2013;Colosimo et al, 2013). Regarding rAF-MSCs, there are reports demonstrating their isolation and characterization by a few molecular markers (Fei et al, 2013;Slamecka and Chrenek, 2013;Kováč et al, 2016) and showing their potential in preclinical research (Steigman et al, 2009;Klein et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Regarding rAF-MSCs, there are reports demonstrating their isolation and characterization by a few molecular markers (Fei et al, 2013;Slamecka and Chrenek, 2013;Kováč et al, 2016) and showing their potential in preclinical research (Steigman et al, 2009;Klein et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Rabbit as a biological model for AF-MSC research was proposed to be very feasible due to cost-effectiveness, logistical needs and common occurrence of large litters (Klein et al, 2010). Furthermore, rabbit mesenchymal stem cells have been shown to possess both cellular and tissue physiology closely resembling that of human MSCs (Fox, 1984;Warden, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit

Kováč¹,

Vašíček²,

Kulíková³

et al. 2017

JCEA

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The isolation of amniotic fluid-derived mesenchymal stem cells (AF-MSCs) has been already shown in human and several other species including rabbit. However, prior to the preclinical research on various animal models it is desirable to define AF-MSCs by a panel of surface protein markers. Therefore, the aim of this preliminary study was to detect the expression of several protein markers on the surface of AF-MSCs isolated from local breed of Nitra rabbit. Amniotic fluid was collected from humanely sacrificed rabbits (n = 3) and AF-MSCs were cultured to a third passage. Flow cytometry was used to detect surface protein marker expression and for viability testing. Rabbit AF-MSCs (rAF-MSCs) were also analyzed by transmission electron microscopy to define the ultrastructure. rAF-MSCs showed both sufficient viability (more than 80%) and low apoptosis rates at third passage and highly expressed CD29 (88.17 ± 7.17%) and CD44 (80.00 ± 2.28%). However, a dim expression of CD90 (17.24 ± 1.31%) and negative expression of CD73 (1.21 ± 0.56% and 4.41 ± 1.46%), CD105 (1.67 ± 0.37%) and CD166 (0.96 ± 2.26%) was observed. Additionally, ultrastructure analysis revealed eccentrically located nucleoli, an abundance of thin pseudopodia on cells' surfaces and proved the presence of typical mesenchymal stem cell features. In conclusion, this set of data contributes to more detailed information on rAF-MSCs, which were previously proposed feasible for preclinical stem cell research and as a suitable source for the cryopreservation of animal genetic resources in gene bank.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit

Kováč¹,

Vašíček²,

Kulíková³

et al. 2017

JCEA

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Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta

Steigman

Fauza

2015

CP Stem Cell Biology

Self Cite

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Diverse progenitor cell populations, including mesenchymal, hematopoietic, trophoblastic, and possibly more primitive stem cells can be isolated from the amniotic fluid and the placenta. At least some of the amniotic and placental cells share a common origin, namely the inner cell mass of the morula. Indeed, most types of progenitor cells that can be isolated from these two sources share many characteristics. This unit will focus solely on the mesenchymal stem cells, the most abundant progenitor cell population found therein and, unlike some of the other stem cell types, present all through gestation. Protocols for isolation, expansion, freezing, and thawing of these cells are presented. Preference is given to the simplest methods available for any given procedure. © 2015 by John Wiley & Sons, Inc.

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Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells

Kováč

Vašíček

Kulíková

et al. 2017

Biotechnology Progress

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Over the years, there has been much confusion in defining molecular markers of mesenchymal stem cells (MSCs) for other than human species due to a lack of species-specific antibodies. Therefore, the aim of our study was to define rabbit amniotic fluid-derived mesenchymal stem cells (rAF-MSCs) and to reflect upon the current identification of AF-MSCs by providing a summary of detected surface markers in different species. The expression of rAF-MSC surface markers was analyzed at the protein and mRNA level. Flow cytometry analyses showed that rAF-MSCs were positive for CD29 and CD44, low positive for CD90, but negative for CD73, CD105, and CD166. Interestingly, RT-PCR (reverse transcription-polymerase chain reaction) exposed a discprepancy between transcribed mRNA and protein expression, as the cells expressed mRNA of all MSC markers: CD29, CD44, CD73, CD90, CD105, and CD166. Our results also confirmed the mesenchymal nature of isolated cells by morphology, ultrastructure, and intracellular marker expression profile. In addition, the expression of few pluripotency markers was also detected. We also found that passaging did not affect apoptosis and viability. Similarly, changes in karyotype were not observed during passaging. In conclusion, the provided characteristics may be used as a comprehensive set of criteria to define and characterize rAF-MSCs, required for the identification of these cells in preclinical investigations. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1601-1613, 2017.

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Chest wall repair with engineered fetal bone grafts: an efficacy analysis in an autologous leporine model

Cited by 33 publications

References 25 publications

Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit

Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit

Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta

Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells

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